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All Journal JURNAL KIMIA SAINS DAN APLIKASI Sebatik HERBAPHARMA : Journal of Herb Farmacological Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Borobudur Pharmacy Review
Luviriani, Eva
STIKES An Nasher Cirebon
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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KONSENTRASI HAMBAT DAN BUNUH MINIMUM EKSTRAK ETANOL DAN METANOL KULIT DURIAN (Durio zibethinus Murr) TERHADAP SALMONELLA THYPI Rosellynia Calyptranti; Eva Luviriani; Linda Safitri
HERBAPHARMA : Journal of Herb Farmacological Vol 4 No 1 (2022): Volume 4 Nomor 1 Juni 2022
Publisher : STIKes Muhammadiyah Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55093/herbapharma.v4i1.285

Abstract

Durian (Durio zibethinus Murr) is a type of fruit that is very popular by everyone and is classified as a very abundant fruit. Compound content active in durian skin (Durio zibethinus Murr) such as alkaloids, phenols, flavonoids, tannins, and saponins can inhibit and kill bacteria that function as the antibacterial. The active compound can be obtained through the extraction process with a suitable solvent. This study aims to determine the value and difference between Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Durian Skin ((Durio zibethinus Murr.) Ethanol and Methanol Extract against Salmonella typhi. Salmonella typhi is a pathogenic bacterium that causes a large number of infections in humans in the form of typhoid fever. This study uses a completely randomized design (CDR). A factorial pattern consisting of 2 factors, namely the solvent factor and the concentration factor. Data analysis used Two Way ANOVA test to find out the comparison of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Durian Skin (Durio zibethinus Murr.) Ethanol and Methanol Extract against Salmonella typhi. If the data is not normally distributed then followed by a nonparametric statistical test, namely the Kruskal-Wallis test. The result of this study showed that at multilevel dilutions of Salmonella typhi which shows the number of colonies 30-300 CFU/ml at a dilution of 10-7. Durian peel ethanol extract can inhibit Salmonella typhi bacteria in the concentration of 25% and durian peel methanol extract can inhibit bacteria Salmonella typhi at a concentration of 10%. Statistical test results show that there is no difference in the Minimum Inhibitory Concentration (MIC) of Durian Skin ((Durio zibethinus Murr.) Ethanol and Methanol Extract against Salmonella typhi. (Asymp sig. >0,05).
Activity of Actinomycetes Isolated from Mangrove Segara Anakan Cilacap toward Methicillin-resistant Staphylococcus aureus (MRSA) Ari Asnani; Eva Luviriani; Oedjijono Oedjijono
Jurnal Kimia Sains dan Aplikasi Vol 23, No 1 (2020): Volume 23 Issue 1 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2815.136 KB) | DOI: 10.14710/jksa.23.1.1-7

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a major health concern because it causes numerous infections in both healthcare facilities and communities. The development of multiresistant against topical antibiotics has caused substantial difficulty in the management of Staphylococcus infection. Thus, this research was aimed to explore indigenous marine Actinomycetes isolated from Segara Anakan Cilacap for anti-MRSA activity. The methods used were screening anti-MRSA activity using agar-block method, production of anti-MRSA extract, extraction of the anti-MRSA extract with ethyl acetate, MIC determination of the ethyl-acetate extract, and species identification based on morphology and 16S rRNA genes. The results indicated that 14 out of 16 Actinomycetes have anti-MRSA activity. Three isolates, which were W-5B, W-5A, and P-7D, showed the highest anti-MRSA activity with the inhibition zone of 2.40 mm, 1.20 mm, and 0.80 mm, respectively. The minimum inhibitory concentration (MIC) of ethyl acetate extract from isolates W-5B, W-5A, and P-7D against MRSA were 2 mg/mL, 4 mg/mL, and 8 mg/mL, respectively. The species identification based on 16S rRNA genes indicated that Actinomycetes W-5B isolate has 92.51% similarity with Streptomyces sp. 2011. The W-5A has 94.69% similarity with Arthrobacter sp. HZ11. The P-7D has 94.79% similarity with Streptomyces clavuligerus strain A-ZN-05. The present study concludes that marine Actinomycetes from sediment mangrove in Segara Anakan Cilacap, Indonesia, has potency as anti-MRSA.
Pemanfaatan Tanaman Obat Sebagai Anti-nyamuk Dan Pemberdayaan Masyarakat Kelurahan Kaliwadas, Kecamatan Sumber, Cirebon Luviriani, Eva; Aji, Rizal Ibrahim; Haqoiroh, Haqoiroh; Hariningsih, Yetti; Azhar, Amelia Jagaddhita; Ramadhan, Alman Shafly; Hermawan, Hildan Bagus; Azizah, Wafik; Fadliyah, Malikhatul; Widiawati, Erika; Habibah, Winda Akhla; Khasanah, Uswatun; Jumaroh, Jumaroh; Nurhaeni, Nurhaeni
Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN Vol. 5 No. 2 (2024)
Publisher : Faculty of Mathematics and Natural Sciences - Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpkmt.v5i2.156

Abstract

Kasus Demam Berdarah Dengue (DBD) di Desa Kaliwadas tergolong tinggi. Faktor-faktor yang menyebabkannya antara lain perkembangbiakan nyamuk Aedes aegypti, iklim, kepadatan penduduk, pengetahuan tentang cara pencegahan penyakit, dan perilaku sosial. Penggunaan obat nyamuk kimia dapat menimbulkan masalah kesehatan dan lingkungan. Tanaman serai wangi (Cymbopogon nardus L.) dapat diekstrak sebagai bahan anti nyamuk ramah lingkungan. Oleh karena itu, perlu dilaksanakan program pemberdayaan dan pemanfaatan tanaman pengusir nyamuk di Desa Kaliwadas untuk meningkatkan kesehatan dan perekonomian masyarakat melalui kegiatan penyuluhan, pelatihan budidaya serai wangi, pembuatan produk, dan pemasaran. Hasil dari program ini menunjukkan adanya peningkatan pengetahuan tentang demam berdarah, pengobatan, pencegahan penyakit, dan pemanfaatan serai wangi. Masyarakat juga mampu membudidayakan tanaman serai wangi dan membuat produk biolarvasida serta semprotan pengusir nyamuk dari tanaman tersebut.
Mangiferin Content in N-Hexane and Diethyl Ether Fractions of Mango (Mangifera indica L.) Leaves Var. Gedong Gincu : Luviriani, Eva; Calyptranti, Rosellynia; Jehan, Nabilah Nauli
Sebatik Vol. 28 No. 2 (2024): December 2024
Publisher : STMIK Widya Cipta Dharma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46984/sebatik.v28i2.2513

Abstract

Mango (Mangifera indica L.) is a tropical fruit plant with high economic value and potential as a source of phytopharmaceuticals. One notable mango variety is Gedong Gincu, originating from Majalengka Regency, West Java, which contains active compounds such as mangiferin, flavonoids, saponins, and tannins with various therapeutic effects. This study aims to compare the mangiferin content in the n-hexane and diethyl ether fractions of Gedong Gincu mango leaf extract using the Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) method. Mango leaves were extracted with ethanol, followed by fractionation using n-hexane and diethyl ether. The resulting fractions were then analyzed for mangiferin content using RP-HPLC. The weights of the n-hexane and diethyl ether fractions were 13.418 g and 8.21 g, respectively. Phytochemical screening revealed the presence of flavonoids, alkaloids, phenols, tannins, and saponins. RP-HPLC analysis showed differences in mangiferin content between the two fractions, with retention times (RT) of 1.430 minutes for the n-hexane fraction and 1.473 minutes for the diethyl ether fraction, indicating variations in solubility and interaction with the mobile phase. The RP-HPLC quantification revealed that the n-hexane fraction contained 339.5 mg/L of mangiferin, while the diethyl ether fraction contained 547.3 mg/L. This study provides valuable insights into the comparison of extraction solvents for mangiferin, which can be further utilized in the development of herbal formulations.
Kualitas mikrobiologi basis pelembab dari lemak daging Sapi metode low temperature dan bouble boiling berdasarkan angka lempeng total (ALT) Kurniawati, Triani; Luviriani, Eva; Rahasasti, Indri Dwi; Calyptranti, Rosellynia; Shafly, Alman
Borobudur Pharmacy Review Vol 5 No 1 (2025): January- June
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/bphr.v5i1.13560

Abstract

Dry skin is a problem that bothers many people. One of the causes of dry skin is skin dehydration, which can be overcome by using moisturizing cosmetics. Vaseline is a moisturizer commonly used by the public because it does not cause allergies and has good moisturizing capabilities. However, it is derived from non-renewable petroleum, necessitating alternatives. Animal fats can be used as cosmetic moisturizers and are considered safer than chemical substances like zinc oxide. According to BPOM, cosmetic products must pass safety tests such as microbial contamination, heavy metal contamination, and chemical contamination tests. Beef fat contains water and fatty acids that can create a suitable medium for microbial growth. However, temperature and water content affect microbial growth, necessitating further research on the microbiological quality of beef fat using low temperature and double boiling methods. This study aims to determine the microbiological quality of beef fat moisturizer bases using low temperature and double boiling methods through Total Plate Count (TPC) testing. This research is a pre-experimental study with a one-shot case study design. The results show that the TPC values of beef fat using low temperature and double boiling methods are each 1 x 10^4 CFU/g. This indicates that the TPC values of beef fat using low temperature and double boiling methods do not meet BPOM criteria, which is < 500 CFU/g. Keywords: Moisturizer; Beef Tallow; Low Temperature; Double Boiling; Total Plate Count.
Co-Authors Aji, Rizal Ibrahim Ari Asnani Azhar, Amelia Jagaddhita azizah, wafik Calyptranti, Rosellynia Fadliyah, Malikhatul Habibah, Winda Akhla Haqoiroh, Haqoiroh Hariningsih, Yetti Hermawan, Hildan Bagus Indri Dwi Rahasasti Jehan, Nabilah Nauli Jumaroh, Jumaroh Linda Safitri Nurhaeni Nurhaeni Oedjijono Oedjijono Ramadhan, Alman Shafly Rosellynia Calyptranti Sari, Indri Puspita Sembiring, Rinawati Shafly, Alman Triani Kurniawati, Triani Widiawati, Erika