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All Journal PREPOTIF : Jurnal Kesehatan Masyarakat Narra J
Hartono, Tiffany
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Apium graveolens leaf ethanolic extract triggers apoptosis in human tongue cancer cells via caspase-3 and poly(ADP-ribose) polymerase pathways: An in vitro study Sandra, Ferry; Hartono, Tiffany; Hayuningtyas, Ria A.; Ranggaini, Dewi; Halim, Johni; Lee, Kyung H.
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1634

Abstract

Recent advances in cancer treatment have focused on developing alternative therapies with reduced adverse effects. Chemoprevention using natural products derived from plants has gained significant attention. Apium graveolens has demonstrated anticancer properties against various cancer cell types, suggesting its potential efficacy against tongue cancer cells. The aim of this study was to evaluate the cytotoxic effects and mechanisms of action of Apium graveolens leaf ethanolic extract (AGLEE) on the HSC-3 tongue cancer cell line. The leaves were processed and extracted with 70% ethanol to obtain an ethanolic extract. HSC-3 cells were cultured, subjected to starvation, and pre-treated with or without Z-DEVD-FMK, a caspase-3 inhibitor. Subsequently, the cells were treated with or without doxorubicin or varying concentrations of AGLEE. To assess cell viability and apoptosis, MTT and sub-G1 assays were performed. Additionally, treated HSC-3 cells were collected, lysed, and analyzed for levels of cleaved-caspase-3 and cleaved-poly (ADP-ribose) polymerase (cleaved-PARP) using ELISA. The inhibitory concentration (IC50) value of AGLEE for reducing viable HSC-3 cells was determined to be 48.29 μg/mL. AGLEE significantly decreased HSC-3 cell viability and increased the percentage of apoptotic cells. It exhibited a concentration-dependent reduction in cell viability and an increase in apoptosis. Furthermore, the extract elevated the levels of cleaved-caspase-3 and cleaved-PARP in HSC-3 cells. Pre-treatment with Z-DEVD-FMK reduced the levels of cleaved-caspase-3 and cleaved-PARP induced by AGLEE. Taken together, AGLEE could be proposed as a potential natural therapeutic agent by inducing apoptosis through the caspase-3/PARP pathway in tongue cancer cells.
EXPLORING PATIENT SATISFACTION AT A PRIVATE DENTAL CLINIC IN JAKARTA: A QUALITATIVE STUDY Hartono, Tiffany; Pramono, Rudy
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 9 No. 2 (2025): AGUSTUS 2025
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v9i2.46763

Abstract

Penelitian ini bertujuan untuk mengidentifikasi faktor-faktor utama yang memengaruhi kepuasan pasien di Klinik X, sebuah klinik gigi swasta yang berlokasi di Jakarta. Desain studi kasus tunggal kualitatif digunakan untuk mendapatkan pemahaman mendalam mengenai pengalaman dan persepsi pasien. Partisipan dipilih melalui teknik convenience sampling dan diwawancarai secara mendalam menggunakan panduan pertanyaan semi-terstruktur dalam Bahasa Indonesia. Seluruh wawancara direkam, ditranskripsi secara verbatim, dan dianalisis secara tematik untuk mengidentifikasi pola dan tema yang berulang. Persetujuan etik diperoleh dari komite etik yang berwenang. Hasil penelitian menunjukkan bahwa kepuasan pasien dipengaruhi oleh beberapa faktor yang saling berkaitan. Rekomendasi dari mulut ke mulut, terutama dari keluarga dan teman, memiliki peran penting dalam menarik pasien baru. Selain itu, kompetensi dan perilaku komunikasi dokter gigi secara konsisten disebut sebagai elemen utama dalam menciptakan pengalaman positif. Keahlian dan sikap profesional staf pendukung, serta kemampuan mereka dalam berkomunikasi dan membangun hubungan yang baik, turut memberikan kontribusi signifikan. Pasien juga menghargai lingkungan klinik yang bersih, higienis, lengkap dengan fasilitas memadai, serta lokasi yang strategis dan mudah diakses. Efisiensi layanan, termasuk ketepatan waktu dan proses administrasi yang lancar, serta persepsi terhadap nilai yang sebanding dengan biaya, turut meningkatkan kepuasan secara keseluruhan. Dari semua faktor tersebut, kompetensi dan komunikasi dokter gigi, serta profesionalisme staf, dianggap sebagai yang paling berpengaruh.
Co-Authors Ferry Sandra Halim, Johni Hayuningtyas, Ria A. Lee, Kyung H. Pramono, Rudy Ranggaini, Dewi