Diah Setiowati, Retno
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Analisis Single Nucleotide Polymorphism (SNP) Gen Laccase-24 (EgLCC24) dalam Ketahanan Kelapa Sawit (Elaeis guineensis Jacq.) terhadap Ganoderma boninense Nugroho, Syarul; Yuli Rahmadi, Hernawan; Syukur, Muhamad; Widodo, Widodo; Bayuardi Suwarno, Willy; Sri Wening, Sri Wening; Faizah, Rokhana; Diah Setiowati, Retno; Adriwan Siregar, Heri
Jurnal Penelitian Kelapa Sawit Vol 33 No 1 (2025): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v33i1.285

Abstract

Ganoderma boninense attack and its rapid spread in oil palm plantations have caused significant economic losses. The use of Ganoderma resistant plant materials can be a solution, but the allelic segregation that occurs causes not all DxP progeny obtained have the nature of resistance to Ganoderma. The role of molecular markers is needed to assist initial selection of Ganoderma resistant DxP progenies in nurseries. This study aims to identify targeted SNP markers from the Laccase-24 gene (EgLCC24), which is thought to play a role in oil palm resistance to Ganoderma. Three oil palm DxP populations were used in this study, namely populations A, B, and C. Ganoderma screening in nurseries was carried out on these three populations to obtain progeny samples with resistant and susceptible phenotypes, which were then further analyzed. The methods used include SNP analysis and the use of SNAP primers for marker development. The SNP differences obtained from this study led to amino acid changes, but did not cause stop codons. The validation results using SNAP primers on the Laccase-24 gene showed that DxP progeny from populations A, B, and C relatively have moderate allele characteristics of Ganoderma resistance.
Respon Morfologi Bibit Kelapa Sawit Terhadap Pemberian Pupuk Fadli Pangaribuan, Ikhwan; Diah Setiowati, Retno; Wening, Sri; Rahma Pratiwi, Dian; Novandi Ginting, Eko; Mardiana, Cut; Ernayunita, Ernayunita
Jurnal Penelitian Kelapa Sawit Vol 33 No 2 (2025): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v33i2.294

Abstract

Oil palm requires a balanced and sufficient supply of nutrients to achieve optimal results. Elements such as nitrogen (N) and potassium (K) are the most essential macronutrients needed by the plant. The cost of fertilizer application is relatively high, which presents opportunities and challenges in assembling plant materials that are efficient in nutrient utilization. This research aims to investigate the responses of various populations of oil palm plants to fertilizer application treatments. The study was organized using a split-split plot design with three factors: fertilizer type [nitrogen (N1) and potassium (N2)], fertilizer dosage [0% (P0), 100% (P1), 75% (P2), and 50% (P3) of the recommended dosage], and plant material [populations 1-6 (V1-V6)], repeated six times. The treatment with the highest average plant height was observed in P2, where the fertilizer was applied at 75% of the recommended dosage. In contrast, the best fertilizer type and dosage combination was obtained from N1:P2, referring to plants treated with 75% of the recommended nitrogen fertilizer. Regarding the populations used, population V1 exhibited the lowest average plant height, significantly differing from other populations. When considering populations, the average leaf count of populations V2 and V6 significantly differed from others and had the highest averages. However, when considering the interaction with fertilizer type, the N2V6 interaction involving population six treated with potassium fertilizer showed the highest average leaf count and significantly differed from other interactions. Regarding stem diameter, populations V3, V2, V4, and V6 had the largest average stem diameters and significantly differed from V5 and V1. The treatment combinations N2P2V6 (crossing six treated with 75% potassium fertilizer) and N1P3V2 (population two treated with 50% nitrogen fertilizer) had the largest average stem diameters, measuring 8.14 cm and 8.07 cm, respectively. Both significantly differed from the other 46 interactions. On the other hand, the smallest average stem diameter was found in plants with the N2P3V1 interaction, measuring 5.33 cm, and significantly differed from the other 47 interactions.