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Puspitasari, Meyrika Dwi
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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UJI KADAR VITAMIN C DAN AKTIVITAS ANTIOKSIDAN DARI FRAKSI KULIT BUAH MELINJO (Gnetum gnemon L) Ibrahim, Kevvy Buana; Wardana, Fendi Yoga; Prasetiyo, Bagus Dadang; Puspitasari, Meyrika Dwi
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 16 No 1 (2024): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v16i1.2451

Abstract

Melinjo (Gnetum gnemon L) in Indonesia is widely used as a food ingredient but melinjo fruit peel is considered waste because there has been no effort to maximize its utilization and there has been no research details of the active compound content in it. Melinjo fruit peels are thought to have the potential to be a source of vitamin C and natural antioxidants that can suppress the activity of free radicals. The study aimed to determine the vitamin C content and antioxidant activity of melinjo fruit peel fraction. It was conducted from August to September 2023 at the Pharmacognosy and Chemistry Laboratory of ITSK RS DR. Soepraoen. Extraction using maceration method followed fractionation. Determination of vitamin C content using UV-Vis spectrophotometry, while determination of antioxidant activity using the DPPH method. The highest vitamin C content was 7.3483 μg/mL in ethanol fraction, followed by ethyl acetate 7.0285 μg/mL and n-hexane 3.6108 μg/mL. Antioxidant activity results with the highest IC50 value of 279.35 μg/mL in ethyl acetate fraction, followed by ethanol 333.98 μg/mL, and n-hexan 4077.31 μg/mL. Solvents with higher polarity can extract vitamin C compounds from simplisia more efficiently and semi-polar solvents can more effectively attract compounds that have antioxidant activity, the type of polarity of a solvent can affect the number and type of compounds to be extracted according to the polarity of compound. It is recommended that further research test the antioxidant active compounds of ethyl acetate fraction and other methods of testing the antioxidant activity melinjo fruit peel.
UJI ANTIBAKTERI FRAKSI DAUN CIPLUKAN (Physalis angulata L.) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli Puspitasari, Meyrika Dwi; Wardana, Fendi Yoga; Widara, Ratih Tyas; Ibrahim, Kevvy Buana
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 16 No 1 (2024): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v16i1.2452

Abstract

Infectious diseases are disorders that can cause or transmit disease. In 2019, an estimated 13.7 million deaths came from infectious diseases, 5 bacteria that most commonly cause infectious diseases are Staphylococcus aureus, Escherichia coli, Streptococcus pneumoniae, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Ciplukan (Physalis angulata L.) is thought to be used as an antibacterial. This study aimed to test the activity of ciplukan leaf fraction against Staphylococcus aureus and Escherichia coli. This research was conducted in August - October 2023 at 2023 at Pharmacognosy Laboratory and Microbiology Laboratory of ITSK DR. Soepraoen Hospital. This research is an experimental quantitative study with macerated ciplukan leaf samples and then continued fractionation using n-hexane, ethyl acetate, and ethanol. The antibacterial activity test used disc diffusion method with 50% DMSO negative control and 30 μg chloramphenicol positive control. Antibacterial activity is indicated by the diameter of the clear zone around the disc then the results were analysed with One Way ANOVA. The highest result antibacterial activity of 20% ethanol fraction were 8.4725 mm on Staphylococcus aureus and 11.6225 mm on Escherichia coli. In the n-hexane fraction against Escherichia coli, no antibacterial activity was found. Based on the results, it is known that the ethanol fraction of 20% concentration is the fraction that has the most effective antibacterial activity against Staphylococcus aureus and Escherichia coli. Based on One Way ANOVA test in each group of bacteria, significant differences were found. Recommended for future researchers to conduct the same research but using the well diffusion method.
Co-Authors Ibrahim, Kevvy Buana Prasetiyo, Bagus Dadang Ratih Tyas Widara Wardana, Fendi Yoga