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All Journal Jurnal Sain Veteriner Journal of Electronics, Electromedical Engineering, and Medical Informatics Jurnal Aisyah : Jurnal Ilmu Kesehatan Jurnal Kedokteran Hewan
Desem, Muhammad Ibrahim
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Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Modelling of Human Cerebral Blood Vessels for Improved Surgical Training: Image Processing and 3D Printing Jacinda, Reica Diva; Yossy, Nebrisca Patriana; Menik Dwi Kurniatie; Hawar, Ihtifazhuddin; Setiawan, Andreas Wilson; Adidharma, Peter; Prasetya, Mustaqim; Desem, Muhammad Ibrahim; Asmaria, Talitha
Journal of Electronics, Electromedical Engineering, and Medical Informatics Vol 7 No 1 (2025): January
Publisher : Department of Electromedical Engineering, POLTEKKES KEMENKES SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/jeeemi.v7i1.583

Abstract

Human cerebral blood vessels are highly intricate and significantly contribute to brain function support. In the surgical process of these vessels, the neurosurgeons will basically employ magnetic resonance imaging (MRI) as an imaging media to understand the location of the disorder, the anatomical position of vessels, and a guide in the surgical process. However, the usage of MRI data remains a challenge for surgeons in understanding anatomical structures in greater detail, as well as the limitations of training in handling difficult cases. This study aims to provide further technology, combining three-dimensional (3D) image models and 3D printing to accommodate the lack of visualization and pre-operative simulation using MRI data. First, the MRI data would be exported to a software 3D slicer that has the ability to process images with a threshold method to segment the required body parts and generate 3D models. Then, the 3D model of blood vessels would be imprinted using the SLA method to provide the complex anatomical structures of blood vessels. The results from both 3D image modeling and 3D printing have been validated and have dimensions similar to those of the MRI data, indicating that this work is highly accurate. This work significantly helps the surgeons to have a better plan for the surgery steps, identify potential issues before the procedure begins, and develop more precise approaches.
Comparison of Morphology and Protein Profile of Acetone and PBS-Fixed Toxoplasma gondii Tachyzoites for Detection of IgG and IgM Seropositivity VALINATA, SISCA; Cahyaningsih, Umi; Nugraha, Arifin Budiman; Kurniawati, Dyah Ayu; Desem, Muhammad Ibrahim; Fong, Sulinawati
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.103155

Abstract

Toxoplasma gondii is an obligate intracellular protozoan that causes zoonotic diseases. The prevalence of toxoplasmosis in humans in Indonesia ranges from 9.7-70%. The parasite is difficult to detect in tissues; therefore, serological testing is the most common method for detecting antibodies against Toxoplasma. Antibodies are often used as the testing criteria for IgG and IgM antibodies. Previous research has shown that agglutination testing with acetone-fixed tachyzoites was only positive for acute infection. The aim of this study was to examine microscopic changes in acetone-fixed tachyzoites, determine their ability to detect IgG and IgM seropositivity, and detect specific proteins for IgG and IgM against toxoplasmosis. Tachyzoites were fixed with acetone (A) and PBS (P). After fixation, tachyzoites were prepared to observe the morphology and sonicated to obtain Soluble Toxoplasma Antigen (STA). STA was then subjected to SDS page and western blotting. The addition of aseton resulted in morphological and protein changes. Although changes occur, acetone-fixed tachyzoites can still react with IgG and IgM seropositivity. Protein bands that can be used as IgG seropositive markers in western blot testing are bands measuring 20, 24, 27, 73, and 110 kDa, and the combination of acetone fixation with anti-goat IgM conjugate will result in seropositive bands.
Early Marriage and Mental Health: A Case-Control Study of Psychological Outcomes Hartanti, Monica Dwi; Adiningsih, Setyo; Isnawati, Rina; Poetranto, Anna Lystia; Puetri, Nona Rahmaida; Desem, Muhammad Ibrahim; Saputra, Fery Surahman; Hidayat, Yance
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 9, No 2 (2024): September
Publisher : Universitas Aisyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30604/jika.v9i2.2847

Abstract

Background: Bengkulu Province is one of the provinces in Indonesia that has the greatest incidence of early marriages. It is well knowledge that women in this province are more likely to be victims of crimes such as domestic violence, problems during pregnancy, and even death during childbirth. It is possible for moms who enter into early marriages to develop psychiatric illnesses, which can not only be a forerunner to a variety of health impacts in later life but also have the potential to have a detrimental impact on their children and families. Objective: This study seeks to examine the factors related to psychological issues in early marriage in Bengkulu Province. Method: For this investigation, a case-control study design is utilized, and the groups are separated according to geography. The group under consideration is a rural region with a greater rate of marriages occurring at a younger age. The control group, on the other hand, is urban. One hundred and thirty-seven mothers who had been married off at a young age made up the study's target sample. Each of the groups had 137 responses, ensuring that the ratio was one to one. The research instrument that was utilized in this study was a questionnaire, and the application Stata 14 was utilized for both univariate test analysis and logistic regression. Results: The husband's perspective (OR: 6.5; 95% CI: 2.1–13.2; P-value: less than 0.001) and societal stigma (OR: 6.4; 95% CI: 1.1–13.4; P-value: less than 0.001) were significant factors associated with elevated stress levels, correlating with the highest scores. The control group comprised quality of life (OR: 6.7; 95% CI: 2.1-11.9; P-value: 0.001) and family support (OR: 6.2; 95% CI: 2.1-11.4; P-value: less than 0.001). In the depressed symptoms component, the variables that exhibited a significant conclusive association with the highest score were the spouse's perception (OR 6.4; 95% CI: 2.1-11.7; P-value: less than 0.001) and quality of life (OR: 6.2; 95% CI: 1.6-13.1; P-value: less than 0.001). The control group exhibited poor health literacy (OR: 4.3; 95% CI: 1.3-7.7; P-value: 0.0033) and inadequate family support (OR: 4.1; 95% CI: 2.1-7.5; P-value: less than 0.001). Conclusion: As a conclusion, the findings of this study highlight the critical importance of implementing comprehensive strategies to address the issue of child marriage and the mental health repercussions that it can have. In order to effectively address the complex interaction of socioeconomic, cultural, and psychological variables that contribute to early marriage, these approaches need to prioritize prevention, intervention, and support services.
EVALUATION OF B1 GENE TO DETECT Toxoplasma gondii: COMPARISON OF THREE SETS NESTED PCR PRIMER Ekawasti, Fitrine; Azmi, Zul; Subekti, Didik Tulus; desem, muhammad ibrahim; Nugraha, Arifin Budiman; Sadiah, Siti; Cahyaningsih, Umi
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.22251

Abstract

This study aimed to evaluate three sets of B1 gene DNA primer for the diagnosis of Toxoplasma gondii. The DNA of Toxoplasma gondii that stored on liquid nitrogen was isolated using DNAzol reagent. The first step of Polymerase Chain Reaction (PCRs) was performed using external and internal primer sets, respectively, and then nPCR. PCR products sequencing was performed by Apical Science. All sequences were analysed using CLC Sequence Viewer Version 8.0 software and compared to sequence database that deposited in ToxoDB (Toxoplasma gondii genome database) using BLAST (https://toxodb.org/toxo/app). Each B1 gene primer was evaluated by performing single PCR (forward and reverse) and nested PCR reactions. Three sets of B1 gene primer have different amplification precision. According to the results of amplicon sequencing, the primer set #2 has the best amplification precision of B1 gene.
Co-Authors Adidharma, Peter Adiningsih, Setyo Andreas Wilson Setiawan Asmaria, Talitha Azmi, Zul Didik Tulus Subekti Fitrine Ekawasti, Fitrine Fong, Sulinawati Hawar, Ihtifazhuddin Hidayat, Yance Jacinda, Reica Diva Kurniawati, Dyah Ayu Menik Dwi Kurniatie Monica Dwi Hartanti, Monica Dwi Nona Rahmaida Puetri, Nona Rahmaida Nugraha, Arifin Budiman Poetranto, Anna Lystia Prasetya, Mustaqim Rina Isnawati, Rina Sadiah, Siti SAPUTRA, FERY SURAHMAN Umi Cahyaningsih Valinata, Sisca Yossy, Nebrisca Patriana