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All Journal Jurnal Sain Veteriner Journal of Electronics, Electromedical Engineering, and Medical Informatics
Desem, Muhammad Ibrahim
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Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Engineering Veterinary

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Modelling of Human Cerebral Blood Vessels for Improved Surgical Training: Image Processing and 3D Printing Jacinda, Reica Diva; Yossy, Nebrisca Patriana; Menik Dwi Kurniatie; Hawar, Ihtifazhuddin; Setiawan, Andreas Wilson; Adidharma, Peter; Prasetya, Mustaqim; Desem, Muhammad Ibrahim; Asmaria, Talitha
Journal of Electronics, Electromedical Engineering, and Medical Informatics Vol 7 No 1 (2025): January
Publisher : Department of Electromedical Engineering, POLTEKKES KEMENKES SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/jeeemi.v7i1.583

Abstract

Human cerebral blood vessels are highly intricate and significantly contribute to brain function support. In the surgical process of these vessels, the neurosurgeons will basically employ magnetic resonance imaging (MRI) as an imaging media to understand the location of the disorder, the anatomical position of vessels, and a guide in the surgical process. However, the usage of MRI data remains a challenge for surgeons in understanding anatomical structures in greater detail, as well as the limitations of training in handling difficult cases. This study aims to provide further technology, combining three-dimensional (3D) image models and 3D printing to accommodate the lack of visualization and pre-operative simulation using MRI data. First, the MRI data would be exported to a software 3D slicer that has the ability to process images with a threshold method to segment the required body parts and generate 3D models. Then, the 3D model of blood vessels would be imprinted using the SLA method to provide the complex anatomical structures of blood vessels. The results from both 3D image modeling and 3D printing have been validated and have dimensions similar to those of the MRI data, indicating that this work is highly accurate. This work significantly helps the surgeons to have a better plan for the surgery steps, identify potential issues before the procedure begins, and develop more precise approaches.
Comparison of Morphology and Protein Profile of Acetone and PBS-Fixed Toxoplasma gondii Tachyzoites for Detection of IgG and IgM Seropositivity VALINATA, SISCA; Cahyaningsih, Umi; Nugraha, Arifin Budiman; Kurniawati, Dyah Ayu; Desem, Muhammad Ibrahim; Fong, Sulinawati
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.103155

Abstract

Toxoplasma gondii is an obligate intracellular protozoan that causes zoonotic diseases. The prevalence of toxoplasmosis in humans in Indonesia ranges from 9.7-70%. The parasite is difficult to detect in tissues; therefore, serological testing is the most common method for detecting antibodies against Toxoplasma. Antibodies are often used as the testing criteria for IgG and IgM antibodies. Previous research has shown that agglutination testing with acetone-fixed tachyzoites was only positive for acute infection. The aim of this study was to examine microscopic changes in acetone-fixed tachyzoites, determine their ability to detect IgG and IgM seropositivity, and detect specific proteins for IgG and IgM against toxoplasmosis. Tachyzoites were fixed with acetone (A) and PBS (P). After fixation, tachyzoites were prepared to observe the morphology and sonicated to obtain Soluble Toxoplasma Antigen (STA). STA was then subjected to SDS page and western blotting. The addition of aseton resulted in morphological and protein changes. Although changes occur, acetone-fixed tachyzoites can still react with IgG and IgM seropositivity. Protein bands that can be used as IgG seropositive markers in western blot testing are bands measuring 20, 24, 27, 73, and 110 kDa, and the combination of acetone fixation with anti-goat IgM conjugate will result in seropositive bands.
Co-Authors Adidharma, Peter Andreas Wilson Setiawan Asmaria, Talitha Fong, Sulinawati Hawar, Ihtifazhuddin Jacinda, Reica Diva Kurniawati, Dyah Ayu Menik Dwi Kurniatie Nugraha, Arifin Budiman Prasetya, Mustaqim Umi Cahyaningsih Valinata, Sisca Yossy, Nebrisca Patriana