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UTILIZATION OF OIL PALM EMPTY FRUIT BUNCHES ENHANCED WITH MOLASSES FOR XYLITOL PRODUCTION Clarissa, Clarissa; Valerie, Levina; Hadi, Alvinsyah Ramdani Pradipta; Purnamadjaja, Andrew Medha; Valentino, Figo; Kembaren, Riahna
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.7739

Abstract

A minimal oil palm empty fruit bunch (OPEFB) usage encourages increased OPEFB utilization to prevent its loss of economic prospects. The xylose and arabinose constituents of OPEFB’s hemicellulose part can be utilized as raw materials for xylitol production. A high diabetes mellitus and periodontal disease prevalence in Indonesia makes xylitol a safe and helpful low-calorie sweetener alternative. This research explores the OPEFB molasses-supplemented media and compares Moniliella pollinis SP5 and other M. pollinis ethyl methanesulfonate (EMS) mutants in xylitol yields. It was found that M. pollinis SP5 accomplished 27% better performance in OPEFB+15%(v/v) molasses than in OPEFB from 0.48 g/g to 0.61 g/g. In addition, M4, M5, and M6 mutants from EMS mutagenesis generated more xylitol concentration in OPEFB + 15%(v/v) molasses compared to the SP5 strain. These findings provide in-sights into the potential of xylitol manufacture with OPEFB. For future research, it is recommended that xylitol production employing OPEFB be optimized.
Valorization of Oil Palm Empty Fruit Bunch Waste Enhanced with Molasses for Erythritol Production Setiabang, Jeffersen Hosea; Fildy, Michael Ryan; Limawan, Kezia Zefanya; Widiyono, Advent Roan; Yonas, Nathanael Darrell; Nataniel, Jocelyn; Saputra, Indra Kurniawan; Kembaren, Riahna
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.22551

Abstract

Indonesia, a leading palm oil producer, generates 1,250 tons of Oil Palm Empty Fruit Bunches (OPEFB) waste daily, typically disposed of through polluting combustion. This study explores repurposing OPEFB as a substrate for erythritol production, a popular sweetener with minimal impact on blood sugar. The research utilized Moniliella pollinis mutant SP5, a yeast capable of producing erythritol from various substrates. The process involved acid hydrolysis of OPEFB followed by fermentation. When fortified with 15% v/v molasses, OPEFB successfully served as an optimal carbon source for erythritol production, achieving a mass yield of 0.2878 g/g. Further improvement came through the use of ethyl methane sulfonate (EMS) mutagenesis, with the best mutant (mutant 7) reaching a yield mass of 0.3298 g/g and volumetric productivity of 0.0534 g/L/h. This research aims to advance Indonesia's self-sufficiency in erythritol production while providing a sustainable solution for OPEFB waste reduction, simultaneously addressing economic and environmental concerns.
Valorization of Oil Palm Empty Fruit Bunches (OPEFB) for Bioethanol Production in Indonesia Gozal, Celine; Halim, Amelia; Ridwan, Albert; Wong, Sidney Laura; Young, Kenneth Gabriel; Nataniel, Jocelyn; Kembaren, Riahna
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.12099

Abstract

Indonesia, one of the world's largest producers of palm oil, generates large amounts of Oil Palm Empty Fruit Bunches (OPEFB). Its improper disposal can cause air pollution and soil acidification. OPEFB comprises lignocellulose, which can be hydrolyzed into monosaccharides to produce bioethanol. Bioethanol is a versatile product commonly produced using food crops as its raw material, but it can also be produced using lignocellulosic biomass to lessen the competition for food crops and land. Thus, this study aimed to produce bioethanol from OPEFB and enhance its production through adaptive evolution and supplementation with molasses. Adaptive evolution was carried out by exposing the yeast to ethanol concentrations of 3–12% (v/v). Native and adaptive strains are grown in OPEFB media supplemented with PEG and molasses. As a result, bioethanol was successfully produced, reaching around 10% (v/v) using a combination of OPEFB hydrolysate, PEG, and 20% molasses. Moreover, it was found that strains adapted toward 9% ethanol are more resistant to ethanol, as they can grow faster than the native strain. This shows that OPEFB can be effectively valorized for bioethanol production as a solution for OPEFB disposal and establishing a circular economy.
UTILIZATION OF OIL PALM EMPTY FRUIT BUNCHES ENHANCED WITH MOLASSES FOR XYLITOL PRODUCTION Clarissa, Clarissa; Valerie, Levina; Hadi, Alvinsyah Ramdani Pradipta; Purnamadjaja, Andrew Medha; Valentino, Figo; Kembaren, Riahna
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.7739

Abstract

A minimal oil palm empty fruit bunch (OPEFB) usage encourages increased OPEFB utilization to prevent its loss of economic prospects. The xylose and arabinose constituents of OPEFB’s hemicellulose part can be utilized as raw materials for xylitol production. A high diabetes mellitus and periodontal disease prevalence in Indonesia makes xylitol a safe and helpful low-calorie sweetener alternative. This research explores the OPEFB molasses-supplemented media and compares Moniliella pollinis SP5 and other M. pollinis ethyl methanesulfonate (EMS) mutants in xylitol yields. It was found that M. pollinis SP5 accomplished 27% better performance in OPEFB+15%(v/v) molasses than in OPEFB from 0.48 g/g to 0.61 g/g. In addition, M4, M5, and M6 mutants from EMS mutagenesis generated more xylitol concentration in OPEFB + 15%(v/v) molasses compared to the SP5 strain. These findings provide in-sights into the potential of xylitol manufacture with OPEFB. For future research, it is recommended that xylitol production employing OPEFB be optimized.