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All Journal Biology, Medicine, & Natural Product Chemistry Abdimas Galuh : Jurnal Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Amanda, Cornelia
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 1 
Protective Role of Paederia foetida L. Against Hepatic Inflammatory Response in a Mice Model of Escherichia coli-Induced Sepsis Savitri, Lisa; Freitas, Maria Do Carmo Da Costa; Prasetyawan, Fendy; Saristiana, Yuneka; Mebung, Konradus Klala; Amanda, Cornelia
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.363-368

Abstract

Sepsis is a severe medical condition characterized by a systemic immune response to infection, often leading to multi-organ failure and death if not treated effectively. This study aimed to investigate the antiseptic properties of Paederia foetida leaf extract in male mice induced with sepsis using Escherichia coli. A total of 24 mice were divided into six groups: a normal group (no treatment), a negative control group (distilled water), a positive control group (ciprofloxacin), and three treatment groups receiving P. foetida leaf extract at doses of 100, 300, and 500 mg/kg BW. After 15 days of oral administration, liver tissue samples were analyzed for IL-6 expression, a pro-inflammatory cytokine associated with sepsis. The results revealed a significant reduction in IL-6 expression in the treatment groups, particularly at the 500 mg/kg BW dose. This dose showed the most effective anti-inflammatory response, with IL-6 expression levels comparable to those of the positive control group treated with ciprofloxacin. However, the 100 mg/kg BW dose demonstrated minimal effects, similar to the negative control. These findings suggest that P. foetida leaf extract, especially at higher doses, has potential as an anti-inflammatory agent in sepsis management. The bioactive compounds in the extract, including flavonoids, alkaloids, and terpenoids, likely contribute to its efficacy. This study provides preliminary evidence supporting the use of P. foetida as a potential herbal alternative for managing sepsis, but further clinical research is necessary to confirm its therapeutic potential.
Assessment of Escherichia coli Contamination in Drinking Water from Refill Depots Savitri, Lisa; Kholis, Muhammad Akbar Nur; Prasetyawan, Fendy; Saristiana, Yuneka; Amanda, Cornelia; Mebung, Konradus Klala
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.443-446

Abstract

Refill Drinking Water Depots (DAMIU) have become a popular source of drinking water in Indonesia due to their affordability and accessibility. However, concerns about hygiene and microbial contamination persist, particularly in areas with limited health oversight. This study aimed to evaluate the microbiological quality of refilled drinking water in Kediri City by detecting the presence of Escherichia coli as an indicator of fecal contamination. A descriptive, cross-sectional design involving 22 depots selected based on hygiene criteria was employed. Microbiological analysis used the Most Probable Number (MPN) method, with confirmatory tests including IMViC and Eosin Methylene Blue (EMB) media. The results showed that 20 of 22 depots met the microbiological safety standard of 0 CFU/100 mL for E. coli as set by the Indonesian Ministry of Health and national standards (SNI 3554:2015). Two samples (5 and 7) tested positive for E. coli, likely due to environmental contamination, inadequate sanitation, and poor water handling practices. The positive samples demonstrated typical E. coli characteristics in IMViC (++-+) and gas formation in BGLB media. This study underscores the importance of routine surveillance, public health regulation enforcement, and improved hygiene practices in refilled water depots. Ensuring the microbiological safety of refilled drinking water is essential to prevent waterborne diseases such as diarrhea. The findings call for enhanced collaboration between local health offices and depot operators to maintain clean and safe drinking water standards across communities.
Immunomodulatory Activity of Chinese Betel (Peperomia pellucida L.) Extract on the Spleen Histopathology in a Murine Model of Gastroenteritis Savitri, Lisa; Prasetyawan, Fendy; Saristiana, Yuneka; Meri, Meri; Mebung, Konradus Klala; Amanda, Cornelia
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.447-452

Abstract

Gastroenteritis, a prevalent digestive disorder caused by various pathogens including Escherichia coli, remains a global health challenge with significant morbidity and mortality, particularly in low-income countries. The spleen, as a critical immune organ, is often compromised during systemic infections. Peperomia pellucida (L.) , a traditional medicinal herb, is known for its antimicrobial, anti-inflammatory, and antioxidant properties. This study investigates the histopathological effects of P. pellucida leaf ethanol extract on spleen tissue in mice induced with E. coli to model bacterial gastroenteritis. Thirty male Swiss mice were divided into six groups (n=5): normal control, negative control (aquades), positive control (Yakult), and three treatment groups receiving P. pellucida extract at 100, 300, and 500 mg/kg BW. After seven days of pretreatment, mice were orally infected with E. coli (1×10⁶ CFU/mL) for another seven days. Spleen tissues were harvested, fixed, and stained with hematoxylin-eosin for histopathological evaluation focusing on degeneration, necrosis, and PMN infiltration. Statistical analysis was performed using ANOVA followed by LSD tests. The highest dose of P. pellucida extract (500 mg/kg BW) significantly reduced spleen tissue damage, showing decreased degeneration (9.08%), necrosis (6.05%), and PMN infiltration (18.45%) compared to lower doses. The effect was comparable to the positive control. The ethanol extract of P. pellucida demonstrates a dose-dependent protective effect on spleen histopathology in E. coli-induced gastroenteritis in mice, supporting its potential as a natural antiseptic agent.
Histopathological Evaluation of Stomach Protection by Peperomia pellucida L. in Mice with Gastroenteritis Savitri, Lisa; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo; Juwita, Syntia Tanu; Prasetyawan, Fendy; Saristiana, Yuneka; Mebung, Konradus Klala; Amanda, Cornelia
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.535-540

Abstract

Gastroenteritis, a widespread condition characterized by inflammation of the stomach and intestines, poses significant health challenges globally. Conventional treatments primarily focus on symptomatic relief and do not address the underlying gastric mucosal damage. Peperomia pellucida L., a medicinal plant known for its anti-inflammatory and antioxidant properties, has been suggested to possess gastroprotective effects. This study aims to evaluate the histopathological effects of P. pellucida L. on gastric protection in a mouse gastroenteritis model. Male Swiss mice were divided into six groups, receiving different treatments, including the ethanol extract of P. pellucida at varying doses (100, 300, and 500 mg/kgBW), followed by induction of gastroenteritis with Escherichia coli. Histopathological analysis was conducted to observe tissue damage across the treatment groups, including necrosis and cell degeneration. The results revealed that P. pellucida L. exhibited significant gastroprotective effects, particularly at a dose of 500 mg/kgBW, reducing gastric mucosal necrosis and inflammation compared to the control groups. The plant’s secondary metabolites, including flavonoids, tannins, saponins, and alkaloids, contributed to the observed protective effects by enhancing mucus production, reducing gastric acidity, and promoting tissue repair. These findings suggest that P. pellucida L. could be an alternative treatment for gastroenteritis and related gastric conditions, offering a natural approach to managing gastric inflammation and mucosal protection.
Co-Authors Elfred Rinaldo Kasimo, Elfred Rinaldo Fendy Prasetyawan Freitas, Maria Do Carmo Da Costa Juwita, Syntia Tanu Kholis, Muhammad Akbar Nur Mebung, Konradus Klala Meri, Meri Rochmad Krissanjaya savitri, Lisa Yuneka Saristiana