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All Journal Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Indonesian Journal of Pharmacology and Therapy Jurnal Info Kesehatan
Lely Adel Violin Kapitan
Poltekkes Kemenkes Kupang
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology

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Crystal sediment profile in the urine of public transportation drivers on route 2 in Kupang city Ledoh, Yaksem Yohanis; Djuma, Agustina Welhelmina; Kapitan, Lely Adel Violin
Journal of Indonesian Medical Laboratory and Science Vol 5 No 1: April 2024
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v5i1.121

Abstract

Background: Drivers have a profession that requires prolonged sitting, leading to the release of bone calcium into the bloodstream, which can trigger the formation of urinary stones or nephrolithiasis. Urinary stone (nephrolithiasis) is a condition where stones form in the urinary tract. Objectives: The aim of this study was to describe the crystal sediment profile in the urine of public transportation drivers on Route 2 in Kupang City. Materials and Methods: This descriptive study involved 16 public transportation drivers on Route 2 in Kupang City as research subjects. Data were collected through interviews.  Results: The results showed the presence of various crystals in the urine sediment, including Calcium Oxalate crystals (25%), Calcium Carbonate crystals (18.75%), Uric Acid crystals (12.5%), and Amorphous crystals (6.25%).  Conclusions: Based on the characteristics and habits of the respondents, it was found that frequent urinary retention, drinking less than 1 liter of water per day, and consuming supplemented or carbonated drinks were the three main factors affecting urine sedimentation in public transportation drivers on Route 2 in Kupang City.
Total flavonoid content and analgesic activity of ethyl acetate fraction of Indian jujube (Ziziphus mauritiana lamk) leaves Priska Ernestina Tenda; Fatmawati Blegur; Lely A.V Kapitan
Indonesian Journal of Pharmacology and Therapy Vol 3 No 3 (2022)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.4894

Abstract

Indian jujube leaves (Ziziphus mauritiana Lamk) have various potential biological activity. Previous studies have proven that ethanol extracted from Indian jujube (Z. mauritiana Lamk) leaves had analgesic activity. This study aimed to determine the total flavonoid content and analgesic activity of the ethyl acetate fraction of Indian jujube (Z. mauritiana Lamk) leaves ethanol extract. Tested fraction was prepared by maceration using ethanol as solvent followed by fractionation using ethyl acetate. Identification of active compounds was performed using color reaction, and determination of total flavonoids by colorimetric method. Ethyl acetate fraction was further tested for its analgesic activity using the writhing method. Swiss-Webster white male mice were divided into 5 treatment groups consisting of negative control contained sodium-CMC, positive control contained sodium diclofenac, while groups 3-5 were given ethyl acetate fraction of Indian jujube (Z. mauritiana Lamk) leaves with doses of 50, 150, and 200 mg/kg BW. All interventions were administered as a single oral dose. 0.7% (w/v) acetic acid was used as a pain inducer. Analgesic activity was measured by calculating the percentage of writhing protection as a measure of the analgesic effect produced by each intervention. The data were analyzed using one-way Anova to compare analgesic activity between treatment groups. Results showed that ethyl acetate fraction of Indian jujube (Z. mauritiana Lamk) leaves at doses of 50, 150, and 200 mg/kg BW gave an effect that was not significantly different from sodium diclofenac as positive control. The ethyl acetate fraction of Indian jujube (Z. mauritiana Lamk) leaves ethanol extract contained flavonoids, saponin, and tannins with total flavonoid content of 22.41 ± 1.16% w/w. It can be concluded that the ethyl acetate fraction of Indian jujube (Z. mauritiana Lamk) leaves have analgesic activity in mice.
Aktivitas Antimikroba Ekstrak Laos Putih (Alpinia Galangas) Terhadap Bakteri Escericia Coli Dan Salmonella Sp. Lely Adel Violin Kapitan
JURNAL INFO KESEHATAN Vol 15 No 1 (2017): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol15.Iss1.124

Abstract

A study was conducted on antibacterial activity of white Laos ethanol extract (Alpinia galanga S) on the growth of Escherichia coli bacteria and Salmonella sp bacteria. The white Laos ethanol extract used was a white Laos ethanol extract obtained by extraction with 70% ethanol solvent using percolation method. The effectiveness of white laos ethanol extract was tested by a diffusion method using cylinder. The antibacterial activity of the white Laos ethanol extract is known by observing a drag zone in the clear region around the cylinder, then measured using a sliding range. The experiment used Completely Randomized Design with 5 treatments and 3 replications. The data obtained were then analyzed statistically using analysis of variance (ANOVA). The results showed that the value of F Table 1% <F Count. Subsequent tests using the Honest Real Difference Test (BNJ) showed a diversity coefficient of 0.51% <5% indicating there was a marked difference between each treatment being tested. From these results, it can be concluded that the extract of white Laos ethanol has antibacterial activity against the growth of Escherichia coli bacteria starting at 25% concentration with 10.45 mm2 inhibitory zone and Salmonella sp bacteria starting at 25% concentration with 17,45 mm2 inhibit zone. The higher the concentration the wider the barrier zone.
Co-Authors Agustina Welhelmina Djuma Fatmawati Blegur Ledoh, Yaksem Yohanis Priska Ernestina Tenda