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Unveiling the Hidden Patterns: A Dermoscopic Analysis of Vitiligo Lesions at a Tertiary Care Center in Surakarta, Indonesia Sesia Pradestine; Muhammad Eko Irawanto; Osdatilla Esa Putri; Trya Oktaviani; Benedicta Lauda Anandita
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 4 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i4.1258

Background: Vitiligo, a common depigmenting disorder, presents with a variety of clinical manifestations. Dermoscopy, a non-invasive skin imaging technique, has emerged as a valuable tool for evaluating pigmentary disorders. This study aimed to analyze the dermoscopic patterns of vitiligo lesions in a cohort of patients at a tertiary care center in Surakarta, Indonesia, and to correlate these patterns with disease stability. Methods: This cross-sectional study included 20 adult patients diagnosed with vitiligo at the Dermatology and Venereology Outpatient Clinic of Dr. Moewardi Regional General Hospital Surakarta in July 2023. A dermoscopic examination was performed on all patients using a polarized light dermoscope. Dermoscopic features were analyzed and categorized based on the BPLeFoSK criteria (Border, Pigment Network, Lesions, Follicular, Koebner). Disease stability was assessed based on clinical and dermoscopic findings. Results: The majority of patients were female (75%) and between 21-40 years old (65%). All patients exhibited the characteristic "white glow" under dermoscopy. Other common findings included reduced or absent pigment network (40% each), perifollicular hyperpigmentation (30%), and perilesional hyperpigment (30%). Satellite lesions and micro-Koebner phenomenon, indicative of disease activity, were observed in 10% of patients each. Based on these findings, 80% of patients were classified as having stable vitiligo, while 20% had unstable vitiligo. Conclusion: Dermoscopy revealed a spectrum of patterns in vitiligo lesions, with the "white glow" being a universal finding. The majority of patients in this cohort had stable vitiligo. Dermoscopy can aid in assessing disease activity and guiding treatment decisions in vitiligo patients.

Platelet-Rich Plasma-Derived Exosomes Modulate Follicular Regeneration: A Comparative Mechanistic Analysis with Minoxidil in a Preclinical Model of Androgenetic Alopecia Trya Oktaviani; Arie Kusumawardani; Suci Widhiati; Nugrohoaji Dharmawan; Endra Yustin Ellistasari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 11 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i11.1437

Background: The therapeutic armamentarium for androgenetic alopecia (AGA) is limited, with variable efficacy and potential side effects associated with standard treatments like minoxidil. Platelet-rich plasma-derived exosomes (PRP-Exo) represent a novel acellular strategy, offering a concentrated payload of regenerative biomolecules. This study aimed to rigorously evaluate the therapeutic efficacy and underlying mechanisms of PRP-Exo, as a monotherapy and in combination with minoxidil, in a validated murine model of AGA. Methods: A parallel-group, randomized, double-blind, controlled experimental study was conducted. Thirty-two male C57BL/6 mice with testosterone-induced AGA were randomized (n=8/group) to one of four groups: Negative Control (NC), Positive Control (PC; 5% topical minoxidil), Treatment 1 (T1; intradermal PRP-Exo), or Treatment 2 (T2; combination of PRP-Exo and minoxidil). PRP-Exo were characterized by Transmission Electron Microscopy, Nanoparticle Tracking Analysis, and ELISA for marker proteins. After a 14-day treatment period, efficacy was assessed via hair follicle density (HFD), anagen-to-telogen (A/T) ratio, and hair shaft thickness. Mechanistic insight was obtained by quantifying tissue protein levels of Ki-67 and β-catenin by ELISA. Results: All active treatments significantly improved hair regeneration compared to the NC group. The combination therapy (T2) demonstrated the most profound effects across all metrics, showing statistically superior outcomes compared to both minoxidil (PC) and PRP-Exo (T1) monotherapies in HFD (65.8 ± 12.1 vs. 36.2 ± 8.5 and 47.3 ± 10.4 follicles/mm², respectively; p<0.01). Furthermore, T2 treatment led to the highest A/T ratio and hair shaft thickness. ELISA revealed that T2 treatment also resulted in the highest tissue concentrations of the proliferation marker Ki-67 and the Wnt pathway protein β-catenin, suggesting enhanced mitogenic activity and modulation of key developmental pathways. Conclusion: PRP-Exo is a potent hair regenerative agent, significantly outperforming minoxidil in this preclinical model. The combination of PRP-Exo and minoxidil exhibits a synergistic effect, promoting superior follicular regeneration by concurrently stimulating tissue proliferation and upregulating key components of the anagen-promoting Wnt signaling pathway. These findings underscore the significant clinical potential of PRP-Exo as a next-generation therapy for AGA.

Platelet-Rich Plasma-Derived Exosomes Modulate Follicular Regeneration: A Comparative Mechanistic Analysis with Minoxidil in a Preclinical Model of Androgenetic Alopecia Trya Oktaviani; Arie Kusumawardani; Suci Widhiati; Nugrohoaji Dharmawan; Endra Yustin Ellistasari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 11 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i11.1437

Background: The therapeutic armamentarium for androgenetic alopecia (AGA) is limited, with variable efficacy and potential side effects associated with standard treatments like minoxidil. Platelet-rich plasma-derived exosomes (PRP-Exo) represent a novel acellular strategy, offering a concentrated payload of regenerative biomolecules. This study aimed to rigorously evaluate the therapeutic efficacy and underlying mechanisms of PRP-Exo, as a monotherapy and in combination with minoxidil, in a validated murine model of AGA. Methods: A parallel-group, randomized, double-blind, controlled experimental study was conducted. Thirty-two male C57BL/6 mice with testosterone-induced AGA were randomized (n=8/group) to one of four groups: Negative Control (NC), Positive Control (PC; 5% topical minoxidil), Treatment 1 (T1; intradermal PRP-Exo), or Treatment 2 (T2; combination of PRP-Exo and minoxidil). PRP-Exo were characterized by Transmission Electron Microscopy, Nanoparticle Tracking Analysis, and ELISA for marker proteins. After a 14-day treatment period, efficacy was assessed via hair follicle density (HFD), anagen-to-telogen (A/T) ratio, and hair shaft thickness. Mechanistic insight was obtained by quantifying tissue protein levels of Ki-67 and β-catenin by ELISA. Results: All active treatments significantly improved hair regeneration compared to the NC group. The combination therapy (T2) demonstrated the most profound effects across all metrics, showing statistically superior outcomes compared to both minoxidil (PC) and PRP-Exo (T1) monotherapies in HFD (65.8 ± 12.1 vs. 36.2 ± 8.5 and 47.3 ± 10.4 follicles/mm², respectively; p<0.01). Furthermore, T2 treatment led to the highest A/T ratio and hair shaft thickness. ELISA revealed that T2 treatment also resulted in the highest tissue concentrations of the proliferation marker Ki-67 and the Wnt pathway protein β-catenin, suggesting enhanced mitogenic activity and modulation of key developmental pathways. Conclusion: PRP-Exo is a potent hair regenerative agent, significantly outperforming minoxidil in this preclinical model. The combination of PRP-Exo and minoxidil exhibits a synergistic effect, promoting superior follicular regeneration by concurrently stimulating tissue proliferation and upregulating key components of the anagen-promoting Wnt signaling pathway. These findings underscore the significant clinical potential of PRP-Exo as a next-generation therapy for AGA.

Unveiling the Hidden Patterns: A Dermoscopic Analysis of Vitiligo Lesions at a Tertiary Care Center in Surakarta, Indonesia Sesia Pradestine; Muhammad Eko Irawanto; Osdatilla Esa Putri; Trya Oktaviani; Benedicta Lauda Anandita
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 4 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i4.1258

Background: Vitiligo, a common depigmenting disorder, presents with a variety of clinical manifestations. Dermoscopy, a non-invasive skin imaging technique, has emerged as a valuable tool for evaluating pigmentary disorders. This study aimed to analyze the dermoscopic patterns of vitiligo lesions in a cohort of patients at a tertiary care center in Surakarta, Indonesia, and to correlate these patterns with disease stability. Methods: This cross-sectional study included 20 adult patients diagnosed with vitiligo at the Dermatology and Venereology Outpatient Clinic of Dr. Moewardi Regional General Hospital Surakarta in July 2023. A dermoscopic examination was performed on all patients using a polarized light dermoscope. Dermoscopic features were analyzed and categorized based on the BPLeFoSK criteria (Border, Pigment Network, Lesions, Follicular, Koebner). Disease stability was assessed based on clinical and dermoscopic findings. Results: The majority of patients were female (75%) and between 21-40 years old (65%). All patients exhibited the characteristic "white glow" under dermoscopy. Other common findings included reduced or absent pigment network (40% each), perifollicular hyperpigmentation (30%), and perilesional hyperpigment (30%). Satellite lesions and micro-Koebner phenomenon, indicative of disease activity, were observed in 10% of patients each. Based on these findings, 80% of patients were classified as having stable vitiligo, while 20% had unstable vitiligo. Conclusion: Dermoscopy revealed a spectrum of patterns in vitiligo lesions, with the "white glow" being a universal finding. The majority of patients in this cohort had stable vitiligo. Dermoscopy can aid in assessing disease activity and guiding treatment decisions in vitiligo patients.

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