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All Journal Asian Journal of Science, Technology, Engineering, and Art International Journal of Humanities, Education, and Social Sciences
Agyo, David Ata
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An Investigation of the Level of Heavy Metals Contamination of Goat Meat Roasted with Scrap Tyres in Wukari, Nigeria Joseph, Ikwebe; David, Bando Christopher; Agyo, David Ata; Tadawus, Rejoice Habila
International Journal of Humanities, Education, and Social Sciences Vol 2 No 1 (2024): International Journal of Humanities, Education, and Social Sciences
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ijhess.v2i1.2563

Abstract

The present study was undertaken to investigate the potential for heavy metals (Fe, Cr, Cd, Pb, and Cu) contamination of goat meat roasted with scrap tyres in Wukari using atomic absorption spectrophotometer (AAS). Goat meat samples were obtained from two slaughter points (new market and Mammara). The results revealed the mean concentrations of Fe, Cr, and Cu in the unroasted goat meat samples (UMN and UMM) in both new market and Mammara slaughtering points as 1.7600 ± 1.4000, 0.0012 ±0.0000, 0.8700, 3.2500 ± 0.0000, 0.0025 ± 0.0000, and 0.7850 ± 0.0071 mg/kg respectively. Cd and Pb were not detected in any of the samples. The mean concentrations of Fe, Cr, Cd, and Cu in the goat meat roasted with scrap tyres (RMN1 and RMM1) in both slaughtering houses (new market and Mammara) were 4.1300 ± 0.0141, 0.0025 ± 0.0001, 0.0011 ± 0s.0000, 1.1250 ± 0.0071, 5.1500 ± 0.0000, 0.0035 ± 0.0001, 0.0012 ± 0.0000, and 1.0500 ± 0.0000 mg/kg respectively. Pb was not detected in all the samples. Also, the mean concentrations of Fe, Cr, and Cu in the goat meat roasted with firewood (RMN2 and RMM2) in slaughtering houses (new market and Mammara) were 3.7750 ± 0.0000 mg/kg, 0.0013 ± 0.0000, 0.9850 ± 0.0000, 4.9150 ± 0.0071, 0.0026 ± 0.0000, and 0.8650 ± 0.021 mg/kg respectively. Cd was detected in the samples obtained from Mammara (0.0012 ± 0.0000) but was not detected in the samples collected from new market. Pb was not detected in any of the samples obtained from both slaughtering houses. The mean concentrations of Fe and Cu in (UMN, UMM), (RMN1, RMM1), and (RMN2, RMM2) in both slaughtering points were above the maximum permissible level set by (FAO) and (WHO).The result of this present study show that the practice of roasting goat meat with scrap tyres is dangerous because the meat could accumulate high and unacceptable levels of heavy metals that could pose a threat to human lives, and hence, should be outlawed.
Retracted: Hepatoprotective Effects of Fractions of Adansonia Digitata Leaves on Carbon Tetrachloride (CCL4)- Induced Toxicity in Wistar Rats Ejeh, Yakubu Ojochenemi; John, Umaru Isaac; Agyo, David Ata; Shenia, Zephaniah Hannaniah; Sunday, Gabriel; Tsuvini, Jonah; Gideon, David; Tsojon, Tsojon Zechariah; David, Bando Christopher
Asian Journal of Science, Technology, Engineering, and Art Vol 2 No 2 (2024): Asian Journal of Science, Technology, Engineering, and Art
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstea.v2i2.2776

Abstract

Phytochemicals, naturally occurring compounds in plants, offer health benefits to humans. This study aimed to determine the effects of fractions of ethanol extract of Adansonia digitata leaves on carbon tetrachloride (CCl4)-induced toxicity in wistar rats. The extraction was done using absolute ethanol, followed by fractionation with different solvent combination via column chromatography. Elution of extract was done with solvent system by gradually increasing polarity beginning from n-hexane, chloroform, ethyl acetate, methanol, ethanol and finally water. In total, 22 fractions were collected in 200 mL beaker each. The fractions were subjected to total antioxidant analysis using 1, 1-diphenyl-2-picryhydrazyl (DPPH) assay. Total phenolics and total flavonoids content were analyzed by Folin-Ciocalteu. GC-MS, FTIR and HPLC analysis were also carried out. Thirty-six male albino rats were distributed into six groups of six (n = 6). The negative control group received CCl4 only. The standard control group was administered 2 CCl4/kg body weight + 25mg/kg body weight silymarin followed by different doses of ethyl acetate:ethanol fraction-20 mg/kg, 40 mg/kg and 50 mg/kg for 21 days. Induction and treatment were carried out in the beginning of a new week. The animals were fasted for 24 hours, sacrificed and blood samples were collected for biochemical analysis. The administered fraction led to the statistically insignificant (p> 0.05) and statistically significant (p< 0.05) reduction in the levels of Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) respectively, the reduction in the levels of total protein (TP) and albumin (ALB) were statistically not significant (p> 0.05) in the treated rats as compared to the untreated rats. The phytochemical analysis of the extract revealed the presence of antioxidant and phenolics. From the total antioxidant capacity, fraction 6b (ethyl acetate: ethanol) was selected for GC-MS, FTIR and HPLC analysis. The GC-MS analysis of fraction 6b revealed the presence of 14 compounds from which Bis(2-ethylhexyl) phthalate had the highest percentage constituent (48.30%) while while 2-Methyl-Z,Z-3,13-octadecadienol had the lowest relative abundance of 0.14 %. Dichloroacetic acid, tridecyl este, d-Glycero-d-ido-heptose, Phthalic acid, ethyl pentyl ester, Trifluoroacetoxy hexadecane, 2-Hexene, 6-nitro-, 2-Tetradecanol, 3,5-Dimethyl-2-octanol, Cyclopropanecarboxylic acid, 7,11-Hexadecadienal, 9-Octadecenamide, Squalene and E, E-1,9,17-Docasatriene were the other compounds found in the fraction. FTIR spectral analysis of the fraction showed peaks of various functional groups. The HPLC results showed the presence of quercetin in the fraction.
Co-Authors David, Bando Christopher Ejeh, Yakubu Ojochenemi Gideon, David John, Umaru Isaac Joseph, Ikwebe Shenia, Zephaniah Hannaniah Sunday, Gabriel Tadawus, Rejoice Habila Tsojon, Tsojon Zechariah Tsuvini, Jonah