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All Journal Jurnal Biologi Tropis
Rahmawati, Rizka Dwi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Identification of Secondary Metabolite compounds and Testing of Flavonoid Levels in Beluntas Leaves (Pluchea indica L.) Salamah, Forrela Zahwa; Ramadhani, Mieta Widya; Wahidah, Shinta Nuriyah; Rahmawati, Rizka Dwi; Sjakoer, Nour Athiroh Abdoes; Savana, Amartya Gesit; Ramadhan, Majida; Faisal, Faisal; Nafisa, Nafisa
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8473

Abstract

Most Indonesian people still use traditional medicine because Indonesian medicinal plants can potentially be the main ingredient in medicine They contain various types of natural chemical compounds with diverse pharmacological and biodiversity effects. Several plants that usually grow around the yard and are easy to grow are beluntas (Pluchea indica (L.)). Beluntas are consumed as an additional ingredient when eating or processed as a herbal concoction. This study identified secondary metabolite compounds and total flavonoid levels in beluntas leaves. Beluntas leaves (Pluchea indica (L.)) were extracted using ethanol solvents and then tested using several reagents to identify their compound content. The total flavonoid test in the beluntas leaf extract was also conducted by adding a 10% aluminum chloride solution, sodium acetate, and aquadest to the extract sample, then incubated and measured for absorption using UV-Vis spectrophotometry in the wavelength range between 415-440 nm. This study demonstrates that beluntas leaves contain bioactive compounds including steroids, alkaloids, flavonoids, and tannins with a total flavonoid content recorded at 71.567±70.446 mg/g.
Antibacterial Activity Test of Sapodilia Leaf Extract (Manilkara zapota) Against Escherichia coli Bacteria Rahmawati, Rizka Dwi; Faisal; Ramadhan, Majida
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8557

Abstract

Antibacterial refers to a substance or compound capable of hindering bacterial growth or eradicating bacteria by affecting the permeability of harmful microbes, particularly those harmful to humans. Leaves from the sapodilla tree (Manilkara zapota) contain secondary metabolites like tannins, flavonoids and saponins. The goal of this research is to evaluate the antibacterial properties of sapodilla leaf extract in slowing down the proliferation of Escherichia coli bacteria and to identify the concentration of sapodilla leaf extract that effectively inhibits the growth of Escherichia coli. The investigation employed the well diffusion technique with three repetitions. Data analysis was conducted using the One Way ANOVA test. Findings in this study indicated that the sample or treatment displayed a significance value las than 0,05, suggesting a notable difference among sample types in relation to the diameter of the clear zone, and that the concentration also showed a significance value less than 0,05, indicating a significant difference related to the concentration on the results of the clear zone diameter. The findings from this studys suggest that the greatest antibacterial efficacy was observed at concentration of 100%.
Co-Authors Faisal Faisal Faisal Nafisa, Nafisa Nour Athiroh Abdoes Sjakoer Ramadhan, Majida Ramadhani, Mieta Widya Salamah, Forrela Zahwa Savana, Amartya Gesit Wahidah, Shinta Nuriyah