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All Journal Jurnal Biologi Tropis JSMARTech : Journal of Smart Bioprospecting and Technology
Ramadhani, Mieta Widya
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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Exploration of Secondary Metabolites in Black Pomegranate Peel Extract (Punica granatum L.) as Antioxidants Using DPPH Method Rofiidah, Anisatul; Innaya, Allinha Yusfin; Putri, Nela Vede Rhofa; Maulida, Hilda Jihan; Dewi, Lina Purnama; Al Shofura, Nadya Raisa; Badria, Shoimatul Umi; Faiza, Hilda Wahyu; Hidayah, Ulfa; Ramadhani, Mieta Widya; Rafsanjani, Ramizard; Januarista, Terra; Faisal, Faisal; Ramadhan, Majida
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 2 (2024): JSMARTech Volume 5, No. 2, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.02.46

Abstract

Pomegranate (Punica granatum) is a plant that contains secondary metabolite compounds that can be used as medicinal content. Black pomegranate contains the highest secondary metabolites compared to red pomegranate and white pomegranate, the skin of the pomegranate contains flavonoids, phenols, tannins, and saponins. This study aims to determine the antioxidant activity of black pomegranate peel extract (Punica granatum L.) with the DPPH method. DPPH method is a method to determine the antioxidant activity in the sample by looking at its ability to counteract the free radical compound 2,2-diphenyl-1-picrylhydrazyl. The results of testing the antioxidant activity of black pomegranate skin (Punica granatum L.) produced an IC50 value of 47.51 ppm which can be concluded that black pomegranate skin is classified as very strong. This research is important because there has not been much research on black pomegranate, especially its skin, so there is a need for additional expansion and renewal of the species. In addition, it can be used as the main basis for the advancement of natural materials, especially in the field of health.
Identification of Secondary Metabolite compounds and Testing of Flavonoid Levels in Beluntas Leaves (Pluchea indica L.) Salamah, Forrela Zahwa; Ramadhani, Mieta Widya; Wahidah, Shinta Nuriyah; Rahmawati, Rizka Dwi; Sjakoer, Nour Athiroh Abdoes; Savana, Amartya Gesit; Ramadhan, Majida; Faisal, Faisal; Nafisa, Nafisa
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8473

Abstract

Most Indonesian people still use traditional medicine because Indonesian medicinal plants can potentially be the main ingredient in medicine They contain various types of natural chemical compounds with diverse pharmacological and biodiversity effects. Several plants that usually grow around the yard and are easy to grow are beluntas (Pluchea indica (L.)). Beluntas are consumed as an additional ingredient when eating or processed as a herbal concoction. This study identified secondary metabolite compounds and total flavonoid levels in beluntas leaves. Beluntas leaves (Pluchea indica (L.)) were extracted using ethanol solvents and then tested using several reagents to identify their compound content. The total flavonoid test in the beluntas leaf extract was also conducted by adding a 10% aluminum chloride solution, sodium acetate, and aquadest to the extract sample, then incubated and measured for absorption using UV-Vis spectrophotometry in the wavelength range between 415-440 nm. This study demonstrates that beluntas leaves contain bioactive compounds including steroids, alkaloids, flavonoids, and tannins with a total flavonoid content recorded at 71.567±70.446 mg/g.
Co-Authors Al Shofura, Nadya Raisa Badria, Shoimatul Umi Dewi, Lina Purnama Faisal Faisal Faiza, Hilda Wahyu Hidayah, Ulfa Innaya, Allinha Yusfin Januarista, Terra Maulida, Hilda Jihan Nafisa, Nafisa Nour Athiroh Abdoes Sjakoer Putri, Nela Vede Rhofa Rafsanjani, Ramizard Rahmawati, Rizka Dwi Ramadhan, Majida Rofiidah, Anisatul Salamah, Forrela Zahwa Savana, Amartya Gesit Wahidah, Shinta Nuriyah