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All Journal Padjadjaran Journal of Dentistry
Ganesh, Rajendran
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Demineralized dentin matrix (DDM) from human teeth increases osteoblasts and type i collagen density after tooth extraction: an experimental study Dewi, Renie Kumala; Oktawati, Sri; Gani, Asdar; Suhartono, Eko; Hamrun, Nurlinda; Ganesh, Rajendran; Sapphira, Nadira; Aurenada, Syabita
Padjadjaran Journal of Dentistry Vol 37, No 1 (2025): March 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol37no1.59205

Abstract

Introduction: Post-extraction cavities must be promptly treated to minimize alveolar ridge resorption. Various bone graft materials can be used to encourage bone regeneration in perialveolar defects. Demineralized Dentin Matrix (DDM) is a bone graft material found in human tooth dentin containing type I collagen. The content of DDM is expected to show an increase in mediators that form bone, such as osteoblasts, thereby accelerating the bone healing process. The study aims to analyze the effect of DDM on osteoblast count and type I collagen density during post-extraction bone healing. Methods: This true experimental study used guinea pigs with extracted left mandibular incisors. Sockets were filled with (1) polyethylene glycol gel (control group, n=9) or (2) DDM gel (treatment group, n=9). The gel was inserted into the socket until it was full and then sutured with non-absorbable silk. The guinea pigs were euthanized on days 7, 14, and 21 for osteoblast counting and type I collagen density measurement. One-way ANOVA was used to assess osteoblast numbers, while the Kruskal-Wallis test was applied to analyze type I collagen density. Results: The treatment group exhibited a higher osteoblast count on day 7 (48.73), day 14 (79.00), and day 21 (89.66) compared to the control group (day 7: 33.00, day 14: 59.6, day 21: 78.27). A statistically significant difference was observed between the treatment and control groups in osteoblast count (p = 0.000) and type I collagen density (p = 0.009). Conclusion: DDM increases osteoblast numbers and type I collagen density on days 7, 14, and 21 post-extraction, potentially enhancing bone remodeling
The impact of chitosan derived from black soldier fly (Hermetia illucens) pupae on bone remodeling post-tooth extraction: an in vivo study Maula, Ni’mal; Waty, Marsela Umbar; Dewi, Renie Kumala; Oktawati, Sri; Gani, Asdar; Suhartono, Eko; Ganesh, Rajendran
Padjadjaran Journal of Dentistry Vol 37, No 1 (2025): March 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol37no1.59308

Abstract

Introduction: Bone defects or alveolar sockets commonly occur after tooth extraction. Black Soldier Fly (BSF) pupae contain 35% chitin, which can be converted into chitosan. This study aims to analyze the effect of BSF pupae chitosan gel on the number of osteoblasts and osteoclasts in post-extraction sockets. Method: This study employed a true experimental design. The left mandibular incisor of guinea pigs was extracted. In the control group (n=9), the socket was filled with polyethylene glycol (PEG) gel as a placebo, while in the treatment group (n=9), the socket was filled with BSF pupae chitosan  gel. The gel was applied until the socket was full, followed by suturing with non-absorbable silk. Euthanasia was performed on days 7, 14, and 21 to evaluate the number of osteoblasts and osteoclasts. Data were analyzed using one-way Anova. Results: The osteoblast count in the treatment group increased on day 7 (52.20 ± 1.90), day 14 (91.53 ± 1.00), and day 21 (104.13 ± 5.33) compared to the control group:  day 7 (39.80 ± 5.43), day 14 (61.13 ± 1.10), and day 21 (82.60 ± 2,11). The number of osteoclasts decreased in both groups: in the control group on day 7 (9.83 ± 0.35), day 14 (12.80 ± 0.72), and day 21 (2.46 ± 0.11); and in the treatment group on day 7 (4.86 ± 1.51), day 14 (9 ± 0.34), and day 21 (2.66 ± 0.11). Statistical analysis revealed significant differences in osteoblast and osteoclast counts between the treatment and control groups (p = 0.000). Conclusion: The application of chitosan BSF pupae gel can increase osteoblast numbers and decrease osteoclast numbers after tooth extraction, potentially accelerating bone formation and offering benefits for future bone regeneration.
Co-Authors Asdar Gani, Asdar Aurenada, Syabita Dewi, Renie Kumala Eko Suhartono Hamrun, Nurlinda Maula, Ni’mal Sapphira, Nadira Sri Oktawati, Sri Waty, Marsela Umbar