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W/O/W Emulsions Formula of Bacteriophages ϕPT1b As a Fruit Wash to Inhibit Pathogenic Escherichia coli Contamination Khairunnisa, Icha; Narulita, Erlia; Winarti, Lina; Febrianti, Riska Ayu; Kuswati, Kuswati; Yulian, Ria
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 22, No 1 (2025)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.007143

Abstract

Pathogenic Escherichia coli infect fruit through irrigation contaminated water. Bacteriophage ϕPT1b has ability to infect E. coli and act as food security. Bacteriophage formulations as emulsion preparations increase the efficiency of application and the stability of long-term storage, one of it is W/O/W emulsions. The objective of this study was to examine the effect of cremophor RH 40, span 80 and their interaction on viscosity, droplet size and inhibition of bacteriophage ϕPT1b W/O/W emulsions against pathogenic E. coli. Factorial design method was used to get the optimum formula. The results showed that the cremophor RH 40, span 80, and their interactions increased the viscosity response, decreased the emulsion droplet size, and increased the inhibition against E. coli. FB is the optimum formula for ϕPT1b W/O/W emulsions which has 1% cremophor RH 40 and 4.5% span 80. The formula produced a viscosity of 29 dPas, an average emulsion droplet diameter size of 10.483, and an inhibition against E. coli of 8.65 m. The desirability index value of 0.905 indicates that FB meets 90.5% of the specified criteria.
Hubungan Kekuatan Otot Tungkai Dengan Kecepatan Lari 100 Meter Siswa Kelas XI Budiyanto, Budiyanto; Febrianti, Riska Ayu; Ladjiku, Akram; Rastini, Rastini; Tahir, Nandar; Umar, Is Suleman; Hidayat, Joni Taufik; Ikhsan, Haerul; Ilham, Al
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Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/jrpi.v2i3.31725

Abstract

This study aims to determine how strong the relationship is between leg muscle strength and 100-meter running speed in class XI students at SMA Negeri 3 Gorontalo. The hypothesis in this study is "There is a relationship between leg muscle strength and 100-meter running speed in class XI students at SMA Negeri 3 Gorontalo." The method used in this study is the experimental method, which follows the steps "Pre Test (X1), Treatment (T), and Post Test (X2). The sample determined for this study consisted of 20 class XI students at SMA Negeri 3 Gorontalo, who were selected by Purposive Sampling. The data obtained showed that r count (0.866) was greater than r table (0.444). So, there is a significant relationship between the independent variable and the dependent variable. The data can prove that there is a relationship between leg muscle strength and 100-meter running speed in class XI students at SMA Negeri 3 Gorontalo.
Bacteriophage-Based Wound Care Formulation for Diabetic Ulcers as A Growth Inhibitor of Alcaligenes faecalis Kiswara, Nur Hanifah Alim; Ludfillah, Rayyan Zhafir; Febrianti, Riska Ayu; Narulita, Erlia; Winarti, Lina
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 1 (2026): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i1.44082

Abstract

Diabetic ulcers are frequently complicated by infections from multidrug-resistant bacteria such as Alcaligenes faecalis, necessitating the development of alternative therapeutic strategies. Bacteriophage therapy presents a promising approach due to its specificity and efficacy against antibiotic-resistant pathogens. This study aimed to formulate and evaluate a bacteriophage-based topical cleanser using the phage ϕAFaV1 for treating A. faecalis infections, focusing on high antibacterial efficacy and low dermal toxicity. Three suspension formulas (F1, F2, F3) were prepared with varying chitosan concentrations (0.5; 0.75, and 1.0%) as a suspending agent. These formulations underwent physical stability testing over 28 days, including assessments of viscosity, pH, zeta potential, and homogeneity. Antibacterial efficacy was determined through inhibition zone assays. All three formulations effectively inhibited bacterial growth, forming clear zones. The F2 formula (0.75% chitosan), containing 200 µL of active phage ingredient, was identified as the optimum formulation. It demonstrated superior physical stability and the most effective bactericidal activity against A. faecalis, as confirmed by statistical analysis. These results indicate that a bacteriophage suspension stabilized with 0.75% chitosan is a highly promising and stable topical agent for combating multidrug-resistant A. faecalis in diabetic wound care.
Improving Tuna Chili Product Quality at KWT Larasati through Food Safety Education Febrianti, Riska Ayu; Mujayanah, Ani Rosa Putri Ayu; Segara, Akbar Pandu; Narulita, Erlia; Neliana, Intan Ria; Dliyauddin, Moh
Jurnal Medika: Medika in progres
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/smsftw11

Abstract

Food safety is a crucial aspect of small-scale processed food production, particularly for fish-based products such as tuna chili sauce, which are highly susceptible to contamination and quality deterioration. This community service program aimed to improve the knowledge and awareness of members of Kelompok Wanita Tani (KWT) Larasati regarding the application of food safety principles in tuna chili sauce production. The program was conducted in 2025 at the KWT Larasati production site using an educational and participatory approach, including food safety counseling, interactive discussions, and on-site mentoring and observation. The effectiveness of the program was evaluated using pre-test and post-test instruments to assess changes in participants’ knowledge levels, as well as an observation checklist based on Good Manufacturing Practices for small and micro food enterprises (Cara Produksi Pangan Olahan yang Baik/CPPOB) to evaluate hygiene and sanitation practices. Data were analyzed using descriptive quantitative methods by comparing mean pre-test and post-test scores, and qualitative descriptive analysis of field observations. The results showed a substantial increase in the average food safety knowledge score from 48 to 85 after the educational intervention. Initial improvements were also observed in several food safety practices, including workers’ personal hygiene, equipment cleanliness, production flow arrangement to reduce the risk of cross-contamination, and the completeness of basic product labeling information. Although the implementation of food safety principles has not yet been fully consistent, this program proved effective as an initial step in enhancing food safety awareness and encouraging improvements in production practices. This community service program is expected to serve as a foundation for strengthening the sustainable implementation of CPPOB to improve the quality, safety, and competitiveness of tuna chili products in small-scale food enterprises.