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<![CDATA[UJI ANTIINFLAMASI EKSTRAK METANOL DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav ) PADA TIKUS PUTIH ]]>
<![CDATA[Winarti, Lina]]>;
<![CDATA[Fitriyani, Atik]]>;
<![CDATA[Muslichah, Siti]]>;
<![CDATA[Nuri, Nuri]]>
<![CDATA[ Majalah Obat Tradisional Vol 16, No 1 (2011) ]]>
Publisher : <![CDATA[Faculty of Pharmacy, Universitas Gadjah Mada ]]>
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DOI: 10.14499/mot-TradMedJ16iss1pp%p
<![CDATA[Inflamasi adalah respon alami yang terjadi pada kerusakan jaringan. Untuk menyembuhkan inflamasi orang biasa menggunakan AINS (antiinflamasi non steroid). Antiinflamsi nonsteroid (AINS) yang secara spesifik mempunyai sejarah yang panjang dan banyak menimbulkan kontroversi serta efek samping. Salah satu tanaman obat yang digunakan secara empirik untuk pengobatan secara tradisional adalah sirih merah (Piper Crocatum Ruiz & Pav.). Tanaman ini memiliki potensi untuk dikembangkan menjadi obat untuk antiinflamasi karena mengandung flavonoid, saponin, tanin, dan alkaloid. Tujuan dari penelitian ini adalah untuk meneliti efek antiinflamasi sirih merah (Piper Crocatum Ruiz & Pav.) menggunakan metode induksi karagenin pada tikus. Untuk pengukuran aktivitas antiinflamasi digunakan 5 kelompok yang berbeda, dan sirih merah diberikan dengan dosis 25mg/kgBB, 50mg/kgBB, dan 100mg/kgBB. Asetosal digunakan sebagai kontrol positif. Dari hasil penelitian didapatkan bahwa reduksi inflamasi adalah 77.58% untuk asetosal, 72.3% untuk ekstrak 25mg/kgBB, 85.60% untuk ekstrak 50mg/kgBB, dan 81.02% untuk ekstrak 100mg/kgBB. Ekstrak dengan dosis 50mg/kgBB menunjukkan efek antiinflamasi yang paling besar diantara dosis yang digunakan. Hasil tersebut menunjukkan bahwa ekstrak sirih merah memberikan efek antiinflamasi yang menjanjikan.]]>
<![CDATA[UJI EFEK ANALGETIKA EKSTRAK RIMPANG TEMU KUNCI (Boesenbergia pandurata (Roxb.) Schlechter PADA MENCIT JANTAN GALUR SWISS ]]>
<![CDATA[Winarti, Lina]]>;
<![CDATA[Wantiyah, Wantiyah]]>
<![CDATA[ Majalah Obat Tradisional Vol 16, No 1 (2011) ]]>
Publisher : <![CDATA[Faculty of Pharmacy, Universitas Gadjah Mada ]]>
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DOI: 10.14499/mot-TradMedJ16iss1pp%p
<![CDATA[Penelitian ini dilakukan untuk mengevaluasi efek analgetik ekstrak Boesenbergia pandurata (Roxb.) Schlechter menggunakan metode Witkin et al. Metode ini dilakukan dengan melihat efek penghilangan rasa sakit setelah pemberian asam asetat secara intraperitoneal kepada tikus jantan galur swiss. Efek rasa sakit yang ditimbulkan akibat pemberian asam asetat akan menyebabkan kontraksi dinding sel perut, sampai kepala, kaki yang tertarik ke belakang dan perut yang menyentuh dasar kandang. Simptom ini dinamakan writhing reflex dan dapat dieliminasi dengan analgetik. Analisis dilakukan dengan membandingkan jumlah writhing reflex (geliat) setelah pemberian ekstrak dengan  asetosal sebagai kontrol positif dan aquadest sebagai kontrol negatif. Efek geliat dihitung 30 setelah pemberian asam asetat dengan injeksi intraperioneal. Efek ekstrak Boesenbergia pandurata (Roxb.) Schlechter antara dosis 15, 30 dan 60 mg/kg BB memberikan perbedaan yang signifikan ( P<0.05). Semakin tinggi dosis, semakin tinggi efek yang diberikan. Jumlah geliat pada dosis 30 mg/kg BB tidak berbeda dengan pemberian  asetosal sedangkan dosis 15 dan 60 kg/kgBB berbeda dengan  asetosal. Pemberian dosis 15 mg/kg BB memberikan jumlah geliat yang lebih besar sedangkan pemberian 60 mg/kg BB memberiakn jumlah geliat yang lebih kecil dari  asetosal. Dari perhitungan presentasi proteksi, terdapat perbedaan antar kelompok tetapi dosis 30 dan 60 mg/kgBB tidak berbeda dengan  asetosal. Analisis presentasi efektifitas menujukkan perbedaan antar kelompok kecuali dosis 30 mg/kgBB dengan  asetosal. Secara keseluruhan dapat dikatakan bahwa efek analgesik ekstrak Boesenbergia pandurata (Roxb.) Schlechter bermula pada dosis 30 mg/kgBB yang setara dengan  asetosal. Semakin tinggi dosis, semakin tinggi aktivitasnya dan pemberian ekstrak 60 mg/kgBB memberiak efek lebih besar dari  asetosal.]]>
<![CDATA[PENGARUH KONSUMSI OBAT OLEH IBU HAMIL TERHADAP JANIN YANG DIKANDUNG ]]>
<![CDATA[Winarti, Lina]]>;
<![CDATA[Wisudyaningsih, Budipratiwi]]>
<![CDATA[ IKESMA Vol 3, No 2 (2007) ]]>
Publisher : <![CDATA[FKM - UNEJ ]]>
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<![CDATA[Pregnant woman is possible to expose with disease which need to cure by using drugs. Sometimes a woman suffer from chronic disease should take some medicine, even in pregnant condition. Risk of harm effect happened on consuming drug at pregnant condition depend on type & when the drug given. In first two week, growth of fetus embryo is known by the most critical effect of teratogenic defect because of drug administration. Most critical period of growth of embryo started about 17 day of post impregnation when system organ expanding. It is last until 60 -70 days. Administration of certain drugs on that period can give teratogenic effect. Administration of drug on pregnant woman should be carried with extra attention, because of the possible risk that can occur during a period of pregnancy. If pregnant woman canât avoid not taking drug therapy, she has to counsel with physician to decide what kind of drug she should take. On the other side, before giving drug therapy physician has to consider about drug safety, dosage, usage duration, type of drug & way the drug should given.]]>
<![CDATA[SISTEM PENGHANTARAN OBAT TERTARGET, MACAM, JENIS-JENIS SISTEM PENGHANTARAN, DAN APLIKASINYA ]]>
<![CDATA[Winarti, Lina]]>
<![CDATA[ STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 10, No 2 (2013) ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Jember ]]>
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<![CDATA[Development of methods to improve delivery of drugs for treating dangerous disease like cancer and viral infections are very important today. Selectivity in therapy is needed, hence capability to deliver drugs at specific site being studied in order to reduce toxicity and unwanted side effect at non target site. Many drugs delivery from passive targeting to active targeting delivery will be improved to reach that purpose. Because this system is very important for cancer therapy, many cytotoxic agent have been approved by clinical examination. Besides that targeting drug delivery is very useful for neurological diseases such as alzhemier, liver diseases, kidney, lung, and colon diseases.]]>
<![CDATA[POTENSI PENGGUNAAN KITOSAN RANTAI PENDEK SEBAGAI PEMBAWA DALAM PENGHANTARAN GEN; EVALUASI IN VITRO ]]>
<![CDATA[Winarti, Lina]]>;
<![CDATA[Martien, Ronny]]>;
<![CDATA[Sismindari, S]]>
<![CDATA[ STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 10, No 2 (2013) ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Jember ]]>
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<![CDATA[Gene therapy involves the introduction of DNA or ribonucleic acid (RNA) into the target cell either to express or suppress the biosynthesis of proteins. The success of gene therapy is highly dependent on a suitable carrier system that can efficiently deliver genes in to specific desired cell with minimum cytotoxicity on target cells. Chitosan is interesting to be used as a gene carrier because it has a high positive charge and low toxicity to cells. Positive charge chitosan can form complexes with the plasmid. DNA complexes provide protection against enzymes degradation and promote internalization of plasmids that have been condensed. In this study the complex formation of chitosan-pEFneo-GFP and chitosan / TPP-pEFneo-GFP is by complex coaservation method. The results of complex analysis with a 0.8% agarose gel electrophoresis for 30 minutes 100Volt showed that the complex was stayed in the well and the plasmids did not migrate like plasmids which were dissolved in the water and buffer solvent. Stability evaluation in storage at room temperature for 14 days showed that the complex with chitosan concentration 0.02%-0.04% were the most stable, so that transfection analysis performed for the complex with chitosan concentration of 0.02%-0.04%. Transfection results showed that chitosan is able to protect plasmid and promote the internalization of plasmid into the nucleus and then expressed as a green luminescence. From these results chitosan potentially be developed as a carrier system in gene delivery.]]>
<![CDATA[Review Artikel: PENGGUNAAN FORMULASI NANOPARTIKEL KITOSAN SEBAGAI SISTEM PENGHANTARAN GEN NON VIRAL UNTUK TERAPI GEN ]]>
<![CDATA[Winarti, Lina]]>
<![CDATA[ STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 8, No 3 (2011) ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Jember ]]>
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<![CDATA[Gene therapy is a method to repair damaged or defective genes that responsible for the onset of certain diseases. Now, numerous prototypes of DNA can control the progress of the disease through induction or inhibition of genes, but the bad cellular uptake and the rapid in vivo degradation of DNA-based therapy require the use of delivery system that can facilitate the cellular internalization and keep its activity. Cationic polymers are commonly used as gene carrier because it is easy to form complexes and it has higher stability compared with lipoplexes. Cationic polymer chitosan is the most widely used in gene delivery system because of the low toxicity andbiocompatible. Gene delivery system using viruses shows high transfection result but it has many disadvantages, such as oncogenic effects and immunogenicity. However, cationic polymers, such as chitosan have the potential for complexation of DNA that can be used as a non-viral vector for gene therapy. Chitosan provides a strongelectrostatic interaction with negative charge of DNA to form nanoparticles and effectively protect from nuclease degradation. Those properties make chitosan become a good candidate from non viral gene delivery.]]>
<![CDATA[UJI TERATOGENIK CAMPURAN SERBUK BIJI JINTEN HITAM (Nigella sativa L.), BIJI KELABET (Trigonella foenum-graecum L.), DAN GINSENG (Panax ginseng C. A. Mey.) PADA TIKUS PUTIH GALUR WISTAR ]]>
<![CDATA[Christianty, Fransiska Maria]]>;
<![CDATA[Winarti, Lina]]>
<![CDATA[ STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 9, No 3 (2012) ]]>
Publisher : <![CDATA[Fakultas Kedokteran Gigi Universitas Jember ]]>
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<![CDATA[One of the empirical effects of black cumin seed or jinten hitam (Nigella sativa L.) is the agent of abortivum. Feenugreek seeds should not be prescribed medicinally for pregnant women since they can induce uterine contraction. The objectives of this study are to know whether the flavour mixture of black cumin seed, fenugreek, and ginseng can cause abnormalities or congenital malformation to the rat foetus and also whether the congenital malformation or birth defect have relationship significantly with the dosage given. The mixing was given orally to pregnant rats on the 7th day until 15th day of pregnancy to observe the sum of foetus, living foetus, fetal death, resorption (foetus biometric) and congenital malformation (gross morphology). The results indicated that there were many effects of the mixture toward rat foetus, such as the decrease of weight and length, increase of resorption and fetal death, but statistically not significant, except weight of foetus. The dosage of 520; 1697,8; 5543,3 mg/kg of body weight didnât show abnormalities or congenital malformation.]]>
<![CDATA[FORMULA OPTIMIZATION OF ORALLY DISINTEGRATING TABLET CONTAINING MELOXICAM NANOPARTICLES ]]>
<![CDATA[Winarti, Lina]]>;
<![CDATA[Ameliana, Lidya]]>;
<![CDATA[Nurahmanto, Dwi]]>
<![CDATA[ Indonesian Journal of Pharmacy Vol 28 No 1, 2017 ]]>
Publisher : <![CDATA[Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia ]]>
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DOI: 10.14499/indonesianjpharm28iss1pp53
<![CDATA[Meloxicam is one of oxicams anti-inflamatory drugs that are effective to relieve toothaches, arthritis, dysmenorrhea, and fever. Meloxicam in this study was milled with High Energy Milling (HEM) method to obtain its nano size and then direct compression method was used to produce Orally Disintegrating Tablet (ODT). ODT is designed to be rapidly dissolved on the tongue within a minute. It can be administered without water or chewing and may improve the bioavailability and effectiveness of the drug, and increase the patient’s adherence. The present study aimed to understand the effects of Ac-Di-Sol and Kollidon CL as superdisintegrants, that were used separately or in combination, on the characteristics of nanoparticles meloxicam ODT. It was also to obtain the best proportion of combination between Ac-Di-Sol and Kollidon CL that can produce the optimum formula of meloxicam ODT. The effects of single or combined superdisintegrants were evaluated using Simplex Lattice Design (SLD). Ac-Di-Sol (X1) and Kollidon CL (X2) were the independent variables, while the dependent variables were friability (Y1), disintegrating time (Y2), wetting time (Y3), and percent meloxicam release after 60 seconds (Y4). Optimization of five nanoparticle meloxicam ODT formulas was conducted using Design Expert 7.1.5. The combination of Ac-Di-Sol 4.05mg (X1) and Kollidon CL 10.95mg (X2) in 150mg nanoparticles meloxicam ODT can produce optimal ODT characteristics. After verification there was no difference between predicted value and observed value with p value > 0.05.]]>
<![CDATA[NARINGENIN-LOADED CHITOSAN NANOPARTICLES FORMULATION, AND ITS IN VITRO EVALUATION AGAINST T47D BREAST CANCER CELL LINE ]]>
<![CDATA[Winarti, Lina]]>;
<![CDATA[Ruma Kumala Sari, Lusia Oktora]]>;
<![CDATA[Nugroho, Agung Endro]]>
<![CDATA[ Indonesian Journal of Pharmacy Vol 26 No 3, 2015 ]]>
Publisher : <![CDATA[Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia ]]>
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DOI: 10.14499/indonesianjpharm25iss3pp147
<![CDATA[Naringenin (NAR), a natural flavonoid aglycone of naringin has been extensively investigated for its pharmacological activities, including anti-tumor effects. However, its poor bioavailability has been identified as the single most important challenge in oral drug delivery. Based in this condition, it is used nanoencapsulation to increase the effectiveness of NAR as anti-cancer. The objectives of this research are to develop the formulation of NAR-loaded nanoparticles (NARNPs) as well as to evaluate its potential as anti-cancer against T47D breast cancer cells line. NARNPs is prepared through the method of ionic gelation, meanwhile its characteristic is evaluated through photon correlation spectroscopy (PCS), transmission electron microscopy (TEM), fourier transform infra-red spectroscopy (FTIR), and different scanning calorimeter (DSC). The result of MTT test and cellular uptake indicate that NARNPs increase citotoxicity and internalization of NAR to the cells compared to the free NAR. The result of qualitative apoptosis study using fluorescence microscope indicates that both free NAR and NARNPs are able to induce apoptosis. It can be conclude that Chitosan nanoparticles–TPP conjugates have the capability to encapsulate naringenin hence increase the cellular uptake and cytotoxcicity of naringenin against T47D cell line. NARNPs also can induce the apoptosis effect.Keywords: NAR, Chitosan (CS), ionic gelation, nanoparticles]]>
<![CDATA[FORMULATION OF SELF-NANOEMULSIFYING DRUG DELIVERY SYSTEM OF BOVINE SERUM ALBUMIN USING HLB (HYDROPHILIC-LYPOPHILIC BALANCE) APPROACH ]]>
<![CDATA[Winarti, Lina]]>
<![CDATA[ Indonesian Journal of Pharmacy Vol 27 No 3, 2016 ]]>
Publisher : <![CDATA[Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia ]]>
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DOI: 10.14499/indonesianjpharm27iss3pp117
<![CDATA[Self-Nanoemulsifying Drug Delivery System (SNEDDS) has potential to be developed for oral protein delivery because it is free from water, hence preserving the stability of protein, protecting protein from enzymatic degradation, and enhancing the protein permeability in the gastrointestinal tract (GIT). However, protein-based SNEDDS formulation is challenging due to low solubility property of protein in oil, which is towards zero. This present study aimed to obtain the most compatible SNEDDS system for protein using HLB approach. Bovine serum albumin (BSA) was used as a protein model in this study. A number of 78 formulae with HLB ranging between 11 and 15 were screened to acquire stable SNEDDS composition without the presence of phase separation. Of 13 stable formulae, two were selected (F30, F45) with HLB 15, and then loaded with BSA. Physical characteristics of both formulae were then evaluated and the results suggested that SNEDDS with single hydrophilic surfactant (F45) and HLB 15 was the best formula for protein template as the stability testing showed that phase separation and precipitation did not appear. It was robust to pH and dilution with percentage of transmittance of 96.40±1.05% and the droplet size of 180.9nm. F45 also had uniform distribution of droplets size since the polydispersity index was less than 0.1. The zeta potential of F45 was -0.12mv with loading efficiency 83.57±1.77%. The emulsifying time of F45 was > 2min due to the formation of crystalline gel that was difficult to disperse.]]>
