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All Journal International Journal of Cell and Biomedical Science
Ayu, Dian Respati
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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The Role of Nigella Sativa and Phyllanthus Urinaria L Extracts Enhance Inflammation Cytokine and Growth factor in Mesenchymal Stem Cells Conditioned Medium Ayu, Dian Respati; Irawan, Risky Candra Satria; Prawitasari, Salindri; Shindy, Meirista
International Journal of Cell and Biomedical Science Vol 3 No 7 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i7.48

Abstract

Background: Mesenchymal Stem Cells (MSCs) are cells that have the multipotent ability to undergo self-renewal, differentiate and secrete various bioactive substances, such as chemokines, proteins, microRNAs (miRNAs), growth factors, and cytokines. Conditioned medium of MSC is a medium resulting from cell culture enriched with the secretome of the cultured cells. MSC-CM treated with certain factors can increase the production of growth factors such as VEGF and PDGF, which play a role in angiogenesis and tissue repair. Modification of MSC-CM with bioactive compounds can be a promising strategy to increase the effectiveness of MSCs in medical therapy. Therefore, this study aims to examine whether these herbal extracts can modulate the production of pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, IL-6) and growth factors (SDF-1, PDGF, VEGF) in MSC conditioned medium. Methods: This study used a pre-post research design with four treatment groups. Medium culture of MSCs treated with Nigella sativa (doses of 10 µg/mL) and Phyllanthus urinaria L (doses of 25 µg/mL), which were cultured for 24 and 48 hours. Measurement of cytokine and growth factor levels was carried out using the Enzyme-Linked Immunosorbent Assay (ELISA) method for quantitative analysis. Results: The data reveal distinct patterns in the modulation of protein levels, particularly for SDF-1, IL-1β, IL-6, TNF-α, and IFN-γ, which are critical players in inflammation and tissue regeneration. Conclusion: This study showed that the Nigella sativa and Phyllanthus urinaria L extracts in modifying conditioned medium of Mesenchymal Stem Cells is significant release of pro-inflammatory cytokines and growth factors.
Surface Marker Expression and Morphological Alterations in Umbilical Cord-Derived MSCs Over Passages 4 to 9: A Flow Cytometry and Microscopic Analysis Prawitasari, Salindri; Ayu, Dian Respati; Irawan, Risky Chandra Satria; Prabowo, Adam
International Journal of Cell and Biomedical Science Vol 3 No 9 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i9.51

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Background : Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are promising candidates for regenerative medicine due to their high proliferative capacity, multilineage differentiation potential, and low immunogenicity. However, prolonged in vitro expansion may lead to phenotypic drift and morphological changes that could impact their therapeutic efficacy. This study aimed to evaluate the expression of mesenchymal surface markers (CD73, CD90, CD105) and morphological characteristics of UC-MSCs from passage 4 (P4) to passage 9 (P9). Methods: Flow cytometry was employed using the BD Stemflow™ Human MSC Analysis Kit to quantify the expression of both positive and negative surface markers, while morphological assessments were performed via phase-contrast microscopy. Results : The results revealed that UC-MSCs maintained high expression levels of CD73, CD90, and CD105 across all passages, although a slight decline was observed in later passages. Morphological analysis indicated a transition from spindle-shaped, fibroblast-like cells at early passages to more enlarged and flattened cells with signs of senescence at higher passages. Conclusions : These findings suggest that although UC-MSCs retain their phenotypic identity up to P9, subtle morphological and marker expression changes may occur, underscoring the importance of passage selection in therapeutic applications. This study contributes to the optimization of UC-MSC culture protocols for standardized and effective clinical use.
hUC-MSCs Therapy with EVs Booster Improves Recovery in Stage 2 Chronic Kidney Disease with Hypercholesterolemia : a case report Jutadi; Ayu, Dian Respati; Cahyani, Dini; Arda, Adzani Gaisani; Anggoro, Naufal Sebastian
International Journal of Cell and Biomedical Science Vol 3 No 9 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i9.63

Abstract

Hypercholesterolemia is a common metabolic comorbidity that accelerates the progression of chronic kidney disease (CKD). Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and their secretome, which consist of extracellular vecicles (EVs) and soluble bioactive molecules, have shown potential in modulating inflammation and metabolism. This case report describes significant improvement in serum lipid profile following hUC-MSC and secretome therapy in a patient with stage 2 CKD and hypercholesterolemia. A male patient with CKD stage 2 received two intravenous cycles of hUC-MSC and secretome therapy administered seven months apart. Serial evaluations demonstrated a progressive decline in total cholesterol from 294 mg/dL at baseline to 286 mg/dL after the first treatment and 225 mg/dL after the second. LDL cholesterol decreased from 188 mg/dL to 140 mg/dL, with a mild rebound to 175 mg/dL. HDL cholesterol, initially elevated at 214 mg/dL, showed a modest increase to 220 mg/dL after the first treatment, followed by normalization to 175 mg/dL. Triglyceride levels remained within the normal range (44–51 mg/dL) throughout the observation period. The marked improvement in lipid parameters suggests that hUC-MSC and secretome therapy may exert systemic metabolic regulation via anti-inflammatory, antioxidative, and hepatoprotective mechanisms. hUC-MSC and secretome administration demonstrated potential benefits in lipid homeostasis in a patient with CKD and hypercholesterolemia. These findings support the role of MSC-derived secretome as a promising adjunctive therapeutic approach. Larger controlled trials are warranted to confirm these outcomes and elucidate underlying mechanisms.
Co-Authors Anggoro, Naufal Sebastian Arda, Adzani Gaisani Cahyani, Dini Irawan, Risky Candra Satria Irawan, Risky Chandra Satria Jutadi Prabowo, Adam Prawitasari, Salindri Shindy, Meirista