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All Journal Journal of Biotechnology and Natural Science Jurnal Manajemen International Journal of Cell and Biomedical Science Indonesian Journal of Medical and Pharmaceutical Science
Cahyani, Dini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Analisis Covid-19 Penghambat Ekspor Impor dan Bisnis Antara Indonesia - Cina Yulianty, Neng Novy; Solihah, Destyani Mariah; Rahman, Noor; Cahyani, Dini; Rizki, Ivan Muhammad; Guntari, Sheirany; Kresnayanti, Ari; Firmansyah, Ricky
Jurnal Manajemen Vol 3, No 1 (2023): Februari
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26858/jm.v3i1.44280

Abstract

Ekspor dan Impor merupakan kegiatan ekonomi  kanca perdagangan internasional yang ada di dalam suatu negara untuk menggerakan pertumbuhan  ekonomi dan sumber pendapatan suatu negara. Namun,  apa yang akan terjadi apabila sumber pendapatan dan kebutuhan suatu Negara terhambat karena adanya wabah penyakit mematikan yang hampir seluruh Negara terkena dampaknya dengan jangka waktu penyembuhan yang relatif lama, yang dapat menghambat proses kerjasama internasional , yang membuat nilai Ekspor dan Impor suatu Negara mengalami kenaikan dan penurunan . Maka dari itu dilakukan suatu Penelitian yang  bertujuan untuk mengetahui Analisis Dampak pandemi Covid-19 Terhadap pertumbuhan nilai Ekspor dan Impor Indonesia  -  Cina sejak  sebelum adanya pandemi  Covid-19, pada masa pandemi dan masa setelah pandemi , serta mengamati perdagangan Internasional pada kedua negara dengan cara mengumpulkan data ekspor-impor dari hubungan billateral Indonesia-Cina. Penelitian ini menggunakan metode studi literatur dengan pengumpulan data statistik  yang telah terhitung secara akurat dari beberapa pengamat ekonomi dunia, dan Badan Pusat Statistik (BPS) tahun 2022 . Hasil data yang diperoleh bahwasannya tidak ada perubahan yang signifikan terhadap kegiatan Ekspor-Impor antara Indonesia-China sebelum dan sesudah terjadinya pandemi  Covid-19 Yaitu pada tahun 2020, namun hanya  terjadi sedikit penurunan diawal tahun 2020 hanya sebentar yaitu dibulan Februari, dan mulai mengalami kenaikan di bulan Maret karena kebutuhan akan barang, bahan pokok dan infrastuktur yang tidak bisa di stop serta kerjasama yang erat antar keduanya yang saling mengisi kebutuhan satu sama lain.  Kata kunci :  Analisis, Covid-19, Dampak, Ekspor, Impor, Pertumbuhan
Temporal Dynamics of TNF-α Expression and Cell Viability in LPS-Stimulated Peripheral Blood Mononuclear Cells Cahyani, Dini; Hidayah, Nurul; Sari, Fikriya Novita
International Journal of Cell and Biomedical Science Vol 3 No 7 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i7.49

Abstract

Background: Lipopolysaccharide (LPS), a key component of Gram-negative bacterial membranes, activates innate immune responses through Toll-like receptor 4 (TLR4) signaling in peripheral blood mononuclear cells (PBMCs). This study aimed to evaluate the temporal dynamics of TNF-α expression and cell viability in LPS-stimulated PBMCs to understand the inflammatory and cytotoxic effects of prolonged LPS exposure. Methods: Human PBMCs were treated with increasing concentrations of LPS (10, 30, and 50 ng/mL) for 4, 8, 12, and 24 hours. TNF-α mRNA expression was analyzed using quantitative PCR, while cell viability was assessed via CCK-8 assay and microscopic imaging. Results: LPS stimulation induced a robust, dose-dependent upregulation of TNF-α expression, peaking at 4 hours and gradually declining over time. Concurrently, PBMC viability remained stable up to 12 hours post-stimulation but significantly decreased at 24 hours, particularly at higher LPS concentrations (30–50 ng/mL). Microscopic analysis revealed increased cellular aggregation and morphological changes consistent with immune activation and cytotoxic stress. Conclusion:LPS triggers early TNF-α expression in PBMCs through TLR4-mediated activation of the NF-κB pathway. However, prolonged exposure to LPS results in decreased cell viability, likely due to sustained inflammatory signaling and oxidative stress. These findings provide insight into the dual-phase response of PBMCs to LPS and underscore the importance of tightly regulated inflammation in innate immunity.
The Effect of Arabica Coffee Fruit Skin Extract (Coffea arabica L.) on the Histopathology of Mice (Mus musculus L.) Liver Induced by Ethanol Cahyani, Dini; Maliza, Rita; Setiawan, Haris
Journal of Biotechnology and Natural Science Vol. 1 No. 1 (2021): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.244 KB) | DOI: 10.12928/jbns.v1i1.4734

Abstract

Excessive alcohol consumption can lead to serious live injury. Alcohol is a source of free radicals that can cause oxidative stress. The provision of antioxidants can counter the effects of free radicals that enter the body. The skin of the Arabica coffee fruit contains antioxidant compounds that can be used as a hepatoprotective in the liver. The skin of the Arabica coffe fruit contains antioxidant compounds that may have a hepatoprotective effect on the liver. This study aimed to determine the effect of extract of arabica coffee fruit skin on necrosis, infiltration and haemorrhage in the liver tissue of 15% EtOH-induced mice for 15 days. The animals used for this study were eight weeks old male Balb/c strain mouse weighing approximately 30-40 grams. This research is an experimental study. There were five groups: the control group (K), the 15% ethanol treatment group (E), and the ethanol-induced group and given different doses of arabica coffee skin extract: 125 mg/kg BW. (A), 250 mg / kg BW (B) and 500 mg / kg BW (C). Observation and identification of necrosis, hepatocyte infiltration and haemorrhage were carried out by using Hematoxylin-eosin staining. The data analysis used was One way ANOVA and continued by Duncan Multiple Range Test (DMRT). The histopathological observations showed that the extract of arabica coffee fruit skin at a 250 mg/kg BW dose reduced the number of cell necrosis. There was no infiltration of inflammatory and hemorrhagic cells in the liver of 15% EtOH-induced mice. This study concludes that the extract of the skin of the Arabica coffee fruit has hepatoprotective potential in 15% EtOH-induced mice.
Effector Cytokine Profiles of Ex Vivo Expanded CTLs in Colorectal Cancer HCT-116 Cells Co-Culture Models Ibrahim, Sugeng; Putra, Agung; Hidayah, Nurul; Cahyani, Dini
Indonesian Journal of Medical and Pharmaceutical Science Vol. 4 No. 2 (2025)
Publisher : Sultan Agung Islamic University of Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/ijmps.v4i2.450

Abstract

Background: Colorectal cancer (CRC) remains a major cause of cancer-related mortality worldwide, with limited benefit from conventional therapies in advanced disease. Cytotoxic T lymphocytes (CTLs, CD8⁺ T cells) are critical mediators of anti-tumor immunity, primarily through direct cytotoxicity and cytokine secretion. However, the dual roles of CTL-derived cytokines, particularly interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6), in CRC progression remain incompletely understood. This study aims to analyze the cytokine profile (IFN-γ, TNF-α, and IL-6) in a CTL–CRC cell direct co-culture model. Methods: CD8⁺ T cells were isolated from peripheral blood mononuclear cells (PBMCs) of CRC patients using magnetic negative selection and activated for 5 days with anti-CD3/CD28 beads and IL-2. Purity and viability were assessed by flow cytometry and morphology. Activated CTLs were co-cultured with the HCT116 CRC cell line at effector-to-target (E:T) ratios of 1:1, 5:1, and 10:1 under direct contact for 48 h. Supernatants were collected and cytokine levels (IFN-γ, TNF-α, and IL-6) were quantified using validated sandwich ELISA kits. Results: Direct co-culture with HCT116 cells significantly increased cytokine secretion in an E:T ratio-dependent manner. IFN-γ secretion rose from 3044.6±120 pg/mL at 1:1 to 4882.1±198 pg/mL at 5:1, plateauing thereafter. TNF-α levels remained relatively constant (628.6±67 pg/mL at 1:1 vs. 674.0±91 pg/mL at 10:1). IL-6, nearly undetectable at 1:1 (0.4±0.1 pg/mL), increased dose-dependently to 5.1±0.9 pg/mL at 10:1. Conclusion: Ex vivo expanded CTLs from CRC patients exhibit a distinct cytokine secretion profile characterized by robust IFN-γ release, stable TNF-α production, and a dose-dependent increase in IL-6 across different effector-to-target ratios.
hUC-MSCs Therapy with EVs Booster Improves Recovery in Stage 2 Chronic Kidney Disease with Hypercholesterolemia : a case report Jutadi; Ayu, Dian Respati; Cahyani, Dini; Arda, Adzani Gaisani; Anggoro, Naufal Sebastian
International Journal of Cell and Biomedical Science Vol 3 No 9 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i9.63

Abstract

Hypercholesterolemia is a common metabolic comorbidity that accelerates the progression of chronic kidney disease (CKD). Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and their secretome, which consist of extracellular vecicles (EVs) and soluble bioactive molecules, have shown potential in modulating inflammation and metabolism. This case report describes significant improvement in serum lipid profile following hUC-MSC and secretome therapy in a patient with stage 2 CKD and hypercholesterolemia. A male patient with CKD stage 2 received two intravenous cycles of hUC-MSC and secretome therapy administered seven months apart. Serial evaluations demonstrated a progressive decline in total cholesterol from 294 mg/dL at baseline to 286 mg/dL after the first treatment and 225 mg/dL after the second. LDL cholesterol decreased from 188 mg/dL to 140 mg/dL, with a mild rebound to 175 mg/dL. HDL cholesterol, initially elevated at 214 mg/dL, showed a modest increase to 220 mg/dL after the first treatment, followed by normalization to 175 mg/dL. Triglyceride levels remained within the normal range (44–51 mg/dL) throughout the observation period. The marked improvement in lipid parameters suggests that hUC-MSC and secretome therapy may exert systemic metabolic regulation via anti-inflammatory, antioxidative, and hepatoprotective mechanisms. hUC-MSC and secretome administration demonstrated potential benefits in lipid homeostasis in a patient with CKD and hypercholesterolemia. These findings support the role of MSC-derived secretome as a promising adjunctive therapeutic approach. Larger controlled trials are warranted to confirm these outcomes and elucidate underlying mechanisms.
A Novel Regenerative Approach for Acne Vulgaris Using Combined Umbilical Cord Mesenchymal Stem Cell-Derived Secretome and Platelet-Rich Plasma: A Case Report Zamzam, Salsabillah; Kusumaningrum, Novi; Prawitasari, Salindri; Cahyani, Dini; Adityani, Resanti
International Journal of Cell and Biomedical Science Vol 4 No 11 (2025)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v4i11.71

Abstract

Background: Acne vulgaris is a chronic inflammatory skin disorder that often leads to scarring and pigmentation. Conventional therapies may provide limited improvement and are frequently associated with adverse effects. Recent advances in regenerative medicine suggest that stem cell–derived secretome and platelet-rich plasma (PRP) may promote skin repair and rejuvenation through anti-inflammatory and regenerative pathways. Case Presentation: A 21-year-old male presented with persistent inflammatory acne and post-acne hyperpigmentation. The patient underwent two sessions of combined SH-MSCs-derived secretome and PRP therapy at two-week intervals. Facial assessments were performed using the Janus Skin Analyzer at baseline, day 14, and day 28. Quantitative analysis revealed significant improvement in multiple parameters, including reduction of pore size, pigmentation index, and sebum levels, accompanied by enhanced skin elasticity. Clinically, the patient exhibited visible improvement in overall skin texture, evenness, and clarity without any adverse reactions. Results: Marked clinical improvement was observed after the second session, with visible reduction in acne lesions, fading of post-acne marks, and overall improvement in skin radiance. Quantitative analysis demonstrated a 30% reduction in pore condition, 58% increase in elasticity, 35% decrease in pigmentation index, and 227% reduction in sebum levels compared to baseline. These findings indicate significant enhancement in skin texture, tone, and elasticity. Conclusion: Combination therapy using SH-MSCs-derived secretome and PRP demonstrated promising regenerative and aesthetic outcomes in this patient with acne vulgaris. Larger-scale clinical studies are warranted to validate efficacy and optimize treatment protocols.
Co-Authors Adityani, Resanti Agung Putra Anggoro, Naufal Sebastian Arda, Adzani Gaisani Ayu, Dian Respati Guntari, Sheirany Ibrahim, Sugeng Jutadi Kresnayanti, Ari Kusumaningrum, Novi Maliza, Rita Noor Rahman, Noor NURUL HIDAYAH Nurwijayanti Prawitasari, Salindri Ricky Firmansyah Rizki, Ivan Muhammad Sari, Fikriya Novita Solihah, Destyani Mariah Yulianty, Neng Novy Zamzam, Salsabillah