Article Per Year (5 Year)
p-Index From 2020 - 2025
1.288
P-Index
This Author published in this journals
All Journal International Journal of Cell and Biomedical Science
Prawitasari, Salindri
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
The Effect of X-Ray Radiation to IL-10 Levels in Secretome Mesenchymal Stem Cells Cosmeceutical Product Antari, Arini Dewi; Ghaissani, Shabrina Syifa; Prawitasari, Salindri; Haryono, Erlina; Adityani, Resanti
International Journal of Cell and Biomedical Science Vol 2 No 4 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i4.27

Abstract

Background: X-ray radiation has been widely used in the pharmaceutical industry because it regenerates and repairs damaged tissues. Objective: In this study, we evaluate the effect of X-ray radiation on the secretome cosmeutical product. Methods: We conducted interleukin 10 (IL-10) analysis by ELISA in each product sample after exposure to X-ray radiation. Results: The levels of IL-10 in each sample were significantly lower than those in the control samples. Moreover, the level of IL-10 in the product samples was significantly higher than that of the control sample. Conclusion: In conclusion, exposure to radiation during shipping or storage of skin care products can potentially damage the proteins in the products by inducing the production of reactive oxygen species (ROS) and decreasing the treatment effectiveness.
Therapeutic Potential of Secretome-Derived Wharton's Jelly Mesenchymal Stem Cells in Psoriasis Vulgaris: A Case Study Azzahara, Salma Yasmine; Agustina, Rita; Prawitasari, Salindri
International Journal of Cell and Biomedical Science Vol 2 No 5 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i5.37

Abstract

Background: Psoriasis is a chronic, immune-mediated skin disease that also has systemic manifestations. Case: In this report, we discuss our findings about a 47-years old psoriasis suffering male patient with a Psoriasis Area Severity Index (PASI) score of 10.8, treated with Wharton’s Jelly Mesenchymal Stem Cells-derived Secretome (S-MSCs). Remarkably, complete regression was recorded within a treatment period of a week only. Result: The patient demonstrated a decrease in PASI, from 10.8 to 3.2 after 1 infusion and followed by 4 intramuscular injections of S-MSCs. Bioactive factors secreted by MSCs, cytokines and growth factors, are very likely to be the principal molecules which play a vital role in inflammatory modulation and skin tissue regeneration. No serious adverse events were noted for the patient as a result of secretome infusion and intramuscular injection. Conclusion: This report demonstrates safety and promises to be an effective strategy using S-MSCs treatment for managing the psoriatic issue and, thus, may offer as an alternative approach to overcome the limitations of the cell-based therapy.
Comparison of Two Tangential Flow Filtration Methods in Isolating CD63+/CD9+ Mesenchymal Stem Cell Exosome Putra, Agung; Alif, Iffan; Prasetio, Ardi; Prawitasari, Salindri
International Journal of Cell and Biomedical Science Vol 2 No 4 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Extracellular vesicles, particularly CD63+/CD9+ Mesenchymal Stem Cell Exosome (MSC-Exo), have emerged as crucial mediators of intercellular communication and potential therapeutic agents, including regenerative medicine and immunomodulation. However, the precise isolation and purification of MSC exosomes pose critical challenges. Tangential Flow Filtration (TFF) has gained recognition as an efficient exosome isolation method, offering scalability and versatility. In this study, we address the pressing need for standardized exosome isolation methods by comparing two distinct TFF-based protocols for isolating CD63+/CD9+ MSC exosomes based on filter size pore order. Methods: MSC-Exo were conducted from the Stem Cell and Cancer Research Laboratory (SCCR Indonesia), which were then processed through TFF using different filter sizes and orders. There are two filtration methods compared, first, MSC-Exo was filtered with 1000-5-500-300-100-50-10-5 filter order. Second procedure, MSC-Exo was filtered using 1000-500-300-100-50-10-5 filter order. Result: Flow cytometry analysis revealed variations in the percentage of CD63+/CD9+ in the MSC-Exo based on filter order. The results indicate that the choice of filter order significantly influences the size range with the highest concentration of CD63+/CD9+ MSC-Exo. Conclusion: This research underscores the importance of optimizing TFF-based isolation methods for CD63+/CD9+ MSC exosomes, especially in the order of filter pore size.
The Role of Nigella Sativa and Phyllanthus Urinaria L Extracts Enhance Inflammation Cytokine and Growth factor in Mesenchymal Stem Cells Conditioned Medium Ayu, Dian Respati; Irawan, Risky Candra Satria; Prawitasari, Salindri; Shindy, Meirista
International Journal of Cell and Biomedical Science Vol 3 No 7 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i7.48

Abstract

Background: Mesenchymal Stem Cells (MSCs) are cells that have the multipotent ability to undergo self-renewal, differentiate and secrete various bioactive substances, such as chemokines, proteins, microRNAs (miRNAs), growth factors, and cytokines. Conditioned medium of MSC is a medium resulting from cell culture enriched with the secretome of the cultured cells. MSC-CM treated with certain factors can increase the production of growth factors such as VEGF and PDGF, which play a role in angiogenesis and tissue repair. Modification of MSC-CM with bioactive compounds can be a promising strategy to increase the effectiveness of MSCs in medical therapy. Therefore, this study aims to examine whether these herbal extracts can modulate the production of pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, IL-6) and growth factors (SDF-1, PDGF, VEGF) in MSC conditioned medium. Methods: This study used a pre-post research design with four treatment groups. Medium culture of MSCs treated with Nigella sativa (doses of 10 µg/mL) and Phyllanthus urinaria L (doses of 25 µg/mL), which were cultured for 24 and 48 hours. Measurement of cytokine and growth factor levels was carried out using the Enzyme-Linked Immunosorbent Assay (ELISA) method for quantitative analysis. Results: The data reveal distinct patterns in the modulation of protein levels, particularly for SDF-1, IL-1β, IL-6, TNF-α, and IFN-γ, which are critical players in inflammation and tissue regeneration. Conclusion: This study showed that the Nigella sativa and Phyllanthus urinaria L extracts in modifying conditioned medium of Mesenchymal Stem Cells is significant release of pro-inflammatory cytokines and growth factors.
Surface Marker Expression and Morphological Alterations in Umbilical Cord-Derived MSCs Over Passages 4 to 9: A Flow Cytometry and Microscopic Analysis Prawitasari, Salindri; Ayu, Dian Respati; Irawan, Risky Chandra Satria; Prabowo, Adam
International Journal of Cell and Biomedical Science Vol 3 No 9 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i9.51

Abstract

Background : Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are promising candidates for regenerative medicine due to their high proliferative capacity, multilineage differentiation potential, and low immunogenicity. However, prolonged in vitro expansion may lead to phenotypic drift and morphological changes that could impact their therapeutic efficacy. This study aimed to evaluate the expression of mesenchymal surface markers (CD73, CD90, CD105) and morphological characteristics of UC-MSCs from passage 4 (P4) to passage 9 (P9). Methods: Flow cytometry was employed using the BD Stemflow™ Human MSC Analysis Kit to quantify the expression of both positive and negative surface markers, while morphological assessments were performed via phase-contrast microscopy. Results : The results revealed that UC-MSCs maintained high expression levels of CD73, CD90, and CD105 across all passages, although a slight decline was observed in later passages. Morphological analysis indicated a transition from spindle-shaped, fibroblast-like cells at early passages to more enlarged and flattened cells with signs of senescence at higher passages. Conclusions : These findings suggest that although UC-MSCs retain their phenotypic identity up to P9, subtle morphological and marker expression changes may occur, underscoring the importance of passage selection in therapeutic applications. This study contributes to the optimization of UC-MSC culture protocols for standardized and effective clinical use.
A Novel Regenerative Approach for Acne Vulgaris Using Combined Umbilical Cord Mesenchymal Stem Cell-Derived Secretome and Platelet-Rich Plasma: A Case Report Zamzam, Salsabillah; Kusumaningrum, Novi; Prawitasari, Salindri; Cahyani, Dini; Adityani, Resanti
International Journal of Cell and Biomedical Science Vol 4 No 11 (2025)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v4i11.71

Abstract

Background: Acne vulgaris is a chronic inflammatory skin disorder that often leads to scarring and pigmentation. Conventional therapies may provide limited improvement and are frequently associated with adverse effects. Recent advances in regenerative medicine suggest that stem cell–derived secretome and platelet-rich plasma (PRP) may promote skin repair and rejuvenation through anti-inflammatory and regenerative pathways. Case Presentation: A 21-year-old male presented with persistent inflammatory acne and post-acne hyperpigmentation. The patient underwent two sessions of combined SH-MSCs-derived secretome and PRP therapy at two-week intervals. Facial assessments were performed using the Janus Skin Analyzer at baseline, day 14, and day 28. Quantitative analysis revealed significant improvement in multiple parameters, including reduction of pore size, pigmentation index, and sebum levels, accompanied by enhanced skin elasticity. Clinically, the patient exhibited visible improvement in overall skin texture, evenness, and clarity without any adverse reactions. Results: Marked clinical improvement was observed after the second session, with visible reduction in acne lesions, fading of post-acne marks, and overall improvement in skin radiance. Quantitative analysis demonstrated a 30% reduction in pore condition, 58% increase in elasticity, 35% decrease in pigmentation index, and 227% reduction in sebum levels compared to baseline. These findings indicate significant enhancement in skin texture, tone, and elasticity. Conclusion: Combination therapy using SH-MSCs-derived secretome and PRP demonstrated promising regenerative and aesthetic outcomes in this patient with acne vulgaris. Larger-scale clinical studies are warranted to validate efficacy and optimize treatment protocols.
Secretome-Based Therapy Promotes Epidermal Thickness Recovery and Follicular Regrowth in Fluconazole-Induced Alopecia in Rats Ametati, Holy; Prawitasari, Salindri; Habibi
International Journal of Cell and Biomedical Science Vol 4 No 11 (2025)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v4i11.72

Abstract

Background: Alopecia involves hair loss characterized by decreased follicle density and epidermal thinning, which may be exacerbated by prolonged fluconazole exposure. The secretome derived from human umbilical cord mesenchymal stem cells (hUC-MSCs) possesses anti-inflammatory and regenerative properties that could aid follicular repair. Methods: Twenty-four male Wistar rats were randomly assigned to four groups: control, 5% minoxidil, hUC-MSC secretome, and secretome–minoxidil combination. Alopecia was induced by fluconazole administration for seven days, followed by a seven-day treatment period. Histological evaluation using hematoxylin and eosin staining assessed hair follicle count and epidermal thickness. Results: The hUC-MSC secretome group exhibited a significant increase in follicle count (mean 39.2; p < 0.001) compared with the control and minoxidil groups. However, changes in epidermal thickness were not statistically significant (p = 0.133). Conclusion: hUC-MSC secretome effectively enhances follicular regeneration in fluconazole-induced alopecia and represents a promising biotherapeutic approach for hair restoration.
Co-Authors Adityani, Resanti Agung Putra Agustina, Rita Alif, Iffan Antari, Arini Dewi Ayu, Dian Respati Azzahara, Salma Yasmine Cahyani, Dini Ghaissani, Shabrina Syifa Habibi Haryono, Erlina Holy Ametati Irawan, Risky Candra Satria Irawan, Risky Chandra Satria Kusumaningrum, Novi Prabowo, Adam Prasetio, Ardi Shindy, Meirista Zamzam, Salsabillah