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All Journal COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat International Journal of Cell and Biomedical Science Abdi Kimia : Jurnal Pengabdian Masyarakat Cermin Dunia Kedokteran
Wilaksono, Bhirau
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Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Other

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EDUKASI GERAK SINGKAT, FOKUS, HEBAT: PENGENALAN VILPA (VIGOROUS INTERMITTENT LIFESTYLE ACTIVITY) SEBAGAI STRATEGI PENINGKATAN KONSENTRASI SISWA SMA Hammado, Nurussyariah; Chandrawati, Aida Ayu; Ichsan, Rayhani; Wilaksono, Bhirau; Sarea, Syahrianti
ABDI KIMIA: Jurnal Pengabdian Masyarakat Vol 2 No 2 (2025): Jurnal Edisi Juni
Publisher : Jurusan Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/abdi.v2i2.8477

Abstract

Konsentrasi belajar yang optimal sangat dibutuhkan oleh siswa sekolah menengah untuk mendukung pencapaian akademik. Namun, gaya hidup pasif yang banyak dijumpai pada remaja berdampak pada penurunan fokus dan kesiapan belajar. Kegiatan pengabdian ini bertujuan untuk mengenalkan aktivitas gerak singkat dan intensitas sedang hingga tinggi yang dapat dilakukan dalam rutinitas harian sebagai strategi praktis untuk meningkatkan konsentrasi belajar siswa.Metode pelaksanaan kegiatan terdiri dari tiga tahapan utama, yaitu sesi edukasi mengenai manfaat aktivitas fisik terhadap konsentrasi, praktik gerak singkat terstruktur, serta sesi tanya jawab dan refleksi. Kegiatan dilaksanakan selama satu hari di SMA Negeri 2 Pare-Pare, dengan total 55 peserta.Hasil kegiatan menunjukkan bahwa seluruh siswa mengikuti seluruh rangkaian dengan antusias dan aktif. Siswa-siswa yang mengikuti kegiatan edukasi terlihat responsif saat praktik VILPA dan menunjukkan ketertarikan lebih dalam sesi diskusi dan refleksi. Seluruh peserta menyatakan bahwa kegiatan ini memberikan wawasan baru mengenai pentingnya aktivitas fisik dalam mendukung konsentrasi dan proses belajar.Kegiatan ini memberikan pendekatan baru dalam edukasi kesehatan di sekolah dan membuktikan bahwa keterlibatan langsung siswa dalam kegiatan berbasis gerak dapat mendorong perubahan perilaku menuju gaya hidup lebih aktif dan siap belajar.
Generation of M2c Macrophages Using IL-10 Exposure In Vitro Alif, Iffan; Kurniawan, Dicky Zulfa F; Wilaksono, Bhirau; Susanto, Indra
International Journal of Cell and Biomedical Science Vol 3 No 8 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i8.52

Abstract

Background : Macrophages are essential cells of the innate immune system that highly adaptable and play vital roles in tissue homeostasis, immune regulation, and development. Among their various phenotypes, the M2c subtype—induced by IL-10 and TGF-β—is known for its regulatory functions, high phagocytic capacity, and pro-angiogenic potential. This study aimed to investigate whether M2c polarization via IL-10 stimulation occurs in a dose-dependent manner by differentiating human monocyte-derived macrophages using IL-10 at concentrations of 5, 10, and 20 ng/mL. Methods : IL-10 expression levels were assessed by ELISA and qPCR. The results demonstrated a clear dose-dependent increase in IL-10 expression across both methods. Results : ELISA measurements showed IL-10 levels increasing from a mean of 3.2±0.7 pg/mL in untreated controls to 19.34±2.3 pg/mL (5 ng/mL), 35.5±7 pg/mL (10 ng/mL), and 67.2±5.8 pg/mL (20 ng/mL). Similarly, qPCR analysis revealed a corresponding increase in IL-10 gene expression, with relative fold changes from 1±0 (untreated control) to 3.1±0.4, 6±1.3, and 10.1±1.6, respectively. Conclusions : These findings indicate that IL-10 induces M2c macrophage polarization in a dose-dependent manner, providing insight into optimized conditions for generating regulatory macrophage populations for potential therapeutic applications.
Hypoxia-Induced Modulation of OCT-4, SOX-2, NANOG, and KLF-4 Expression in Mesenchymal Stem Cells Irawan, Risky Chandra Satria; Hidayah, Nurul; Wilaksono, Bhirau
International Journal of Cell and Biomedical Science Vol 3 No 9 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i9.62

Abstract

Background: Mesenchymal stem cells (MSCs) possess remarkable regenerative and immunomodulatory capabilities, largely attributed to their intrinsic stemness properties regulated by transcription factors such as OCT-4, SOX-2, NANOG, and KLF-4. However, in vitro culture under normoxia often leads to stemness decline. Hypoxic preconditioning has been proposed to preserve stemness, though the optimal duration of exposure remains unclear. This study aimed to evaluate the time-dependent effects of hypoxia on the expression of core stemness-related transcription factors in human umbilical cord Wharton’s jelly-derived MSCs. Methods: MSCs (passage 5) obtained from the Stem Cell and Cancer Research (SCCR) Laboratory were cultured under hypoxia (5% O₂) for 0, 4, 6, 10, 12, and 24 hours. Gene expression of OCT-4, SOX-2, NANOG, and KLF-4 was quantified by qRT-PCR using GAPDH as a reference. Morphological changes were observed via phase-contrast microscopy. Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. Results: Hypoxic exposure induced a significant, time-dependent upregulation of OCT-4, SOX-2, and NANOG, with peak expression at 12 hours (approximately 13-, 7-, and 5-fold increases, respectively; p < 0.05). KLF-4 expression showed a modest elevation but was not statistically significant. Prolonged hypoxia (24 hours) resulted in a marked decline in all gene expressions toward baseline levels, accompanied by cell rounding and detachment. These findings indicate that short-term hypoxia enhances MSC stemness, while extended exposure triggers stress-related responses. Conclusion: Controlled short-term hypoxia (approximately 12 hours at 5% O₂) optimally enhances MSC stemness gene expression without compromising morphology or viability. This duration represents an effective preconditioning window for maintaining MSC multipotency and may improve the consistency and therapeutic efficacy of MSC-based applications.
Mental Health and Depression in Indonesia: Risk Factors and Service Utilization for Healthy Aging Using SKI 2023 Data Wilaksono, Bhirau; Damry, Nurul Mufidah; Aminuddin, Nurul Mutiah; Alfiandari, Dhini
COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat Vol. 4 No. 10 (2025): COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mental health conditions, including depression, pose a significant public health challenge in Indonesia, impacting quality of life and healthy aging, particularly among vulnerable populations. This study examines the prevalence of depression and mental health issues, identifies associated risk factors, and evaluates disparities in health service utilization using secondary data from the 2023 Indonesian National Health Survey (SKI 2023). Logistic regression analysis was used to explore associations between mental health outcomes and sociodemographic factors, including age, gender, education, employment, and economic status. The analysis revealed significant geographic and demographic disparities. West Java reported the highest depression prevalence (3.3%) and Bali the lowest (0.3%). Women, unemployed individuals, and those in lower socioeconomic groups exhibited higher prevalence rates. Urban residents had greater access to services compared to rural populations, where stigma and resource limitations were prominent barriers. These findings highlight the urgent need for equitable, community-based mental health initiatives that integrate culturally sensitive care, address social determinants, and reduce stigma. Targeted policies and programs are essential to improving mental well-being, enhancing service accessibility, and supporting healthy aging across Indonesia's diverse populations.
Efektivitas Citicoline sebagai Agen Neuroprotektif dalam Tata Laksana Traumatic Brain Injury Wilaksono, Bhirau; Sompa, Andi Weri
Cermin Dunia Kedokteran Vol 50 No 12 (2023): Penyakit Dalam
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v50i12.1015

Abstract

Background: Traumatic brain injury (TBI) is a condition resulting from physical trauma to the head which can cause structural damage to the brain and interfere with cognitive, motor and emotional functions. Citicoline is a natural compound that plays an important role in phospholipid metabolism and neurotransmitter synthesis in the brain. This evidence-based case report (EBCR) study aims to provide scientific evidence of the potential benefits of citicoline in improving clinical outcomes in TBI patients. Method: Literature search using the PICO format. The formulation of clinical questions was arranged by case. Search for articles on March 6–7th 2023 using the Publish or Perish (PoP) software with the target of searching articles for the last 10 years (2013-2023) on the Scopus, Pubmed, Google Scholar databases, and manual searches on the Cochrane database, using the keyword 'traumatic brain' injury' AND 'therapy' AND 'citicoline' AND 'neuroprotective' AND 'treatment' AND 'outcome' along with synonyms and related terms. Results: Four studies were obtained, with the level of evidence 1a which resulted from a meta-analysis or systematic review of various randomized controlled trials, which were then critically reviewed. Two studies reported no significance of clinical assessment and the Glasgow Coma Scale (GCS); two other studies showed significant results at the level of independence. Conclusion: This study shows that citicoline is beneficial in the independence of TBI patients but does not produce a significant difference in the Glasgow Coma Scale assessment.
Co-Authors Alfiandari, Dhini Alif, Iffan Aminuddin, Nurul Mutiah Andi Weri Sompa Chandrawati, Aida Ayu Damry, Nurul Mufidah Hammado, Nurussyariah Ichsan, Rayhani Irawan, Risky Chandra Satria Kurniawan, Dicky Zulfa F Nurul Hidayah Sarea, Syahrianti Susanto, Indra