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All Journal Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Nurhayani, N
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Computer Science & IT Engineering Medicine & Pharmacology Social Sciences Other

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Effectivity of Rosella Flower Pressed Water (Hibiscus sabdariffa L.) as a Natural Coloring Agent for Examination of Feces Confirmed Helminthiasis Sulistyo, Ayu Indah; Aisyah, Riandini; Nurhayani, N; Mahmudah, Nur
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2024: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.5497

Abstract

Background: Soil Transmitted Helminths (STH) are prevalent in tropics. Eosin 2% dye is commonly used for helminth egg identification, but this dye is carcinogenic and damaging to the environment. Aim: This study evaluates the effectiveness of rosella pressed water (Hibiscus sabdariffa L.) as a natural dye for helminthiasis feces examination, examines its performance with added citric acid, and compares the staining results. Methodology: This experimental study used a post-test only control group design with 27 purposively selected samples of 10% formalin-fixed helminthiasis feces. Staining was performed using rosella pressed water at concentrations of 10%, 20%, 30%, and 40%, with and without 9% citric acid, alongside 2% eosin as a comparison. Observations were made under a 10x microscope, and staining effectiveness was evaluated using a Likert scale by a parasitologist. Results: The staining quality of rosella pressed water was consistent (median = 3) across all concentrations. Parasite visualization varied without citric acid, with reduced quality at 20% concentration (median = 2). Preparation quality remained stable (median = 3). Kruskal-Wallis analysis showed no significant differences (p > 0.05). Discussion: Rosella flowers (Hibiscus sabdariffa L.) contain anthocyanin pigments which are flavonoid compounds that provide red to purple color, so they can be used as a natural dye for STH eggs. Conclusion: This study showed that rosella flower pressed water was effective for STH egg staining, although 2% Eosin gave the best and most consistent results. There were no significant differences between treatments.
Correlation between Socioeconomic Level and Nutritional Status with The Incidence of Helminthiasis in Elementary Students Rahmah, Maulida; Nurhayani, N; Bestari, Rochmadina Suci; Tolibin, Restu Triwulandani; Najmii, Muhammad Firoos Allaam
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2024: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.5520

Abstract

Purpose: To examine the relationship between socioeconomic status and nutritional condition with the prevalence of helminthiasis among students at SDN 02 Selokaton. Methodology: This research employs a quantitative observational analytic approach with a cross-sectional design. A total of 130 fifth- and sixth-grade students were selected using total sampling. The independent variables (socioeconomic status and nutritional status) were assessed through questionnaires and anthropometric measurements, respectively. The dependent variable, helminth infection incidence, was determined through microscopic stool analysis. Data were analyzed using Spearman's test. Results: Among the 65 students who met the inclusion criteria, 20% (13 students) tested positive for helminth infections. Poor nutritional status was found in 6.2% of students, while 43.1% had low nutritional status. Most students came from families with a middle socioeconomic status (78.5%). No significant correlation was found between socioeconomic status or nutritional status and the incidence of helminth infections (p > 0.05). Applications/Originality/Value: Despite the lack of a significant correlation, the high prevalence of helminth infections suggests that factors like environmental hygiene and hygienic behaviors may play a more influential role. Health education and improved sanitation are needed to reduce the prevalence of helminth infections in the area.
Co-Authors Maulida Rahmah, Maulida Najmii, Muhammad Firoos Allaam Nur Mahmudah Riandini Aisyah Rochmadina Suci Bestari Sulistyo, Ayu Indah Tolibin, Restu Triwulandani