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All Journal JUSTER: Jurnal Sains dan Terapan Indonesian Journal of Biomedicine and Clinical Sciences
Rosa Juwita Hesturini
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Biochemistry, Genetics & Molecular Biology Chemistry Energy Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Physics

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Antihyperlipidemic activity of kaffir lime leaf extract (Citrus hystrix DC) on hypercholesterolemic model mice Anjani, Ade Giriayu; Rosa Juwita Hesturini
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 2 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v57i2.16602

Abstract

Kaffir lime (Citrus hystrix DC) is rich in various bioactive substances like flavonoids, tannins, saponins, hesperidin, and several monoterpenes, which are believed to have antihyperlipidemic activity. Hyperlipidemia is a metabolic issue that can lead to high blood pressure and weight gain. This condition is the result of an imbalance in cholesterol levels, including total cholesterol, elevated LDL, and reduced HDL. This study aimed to investigate the antihyperlipidemic activity of kaffir lime leaf extract (C. hystrix DC) on hypercholesterolemic model mice. It was pre- and post-control group design study. Tweenty-five male mice (Mus musculus) weighing 16-21g at 12 wk of age were used in this study. Five mice as normal control mice group (Group 1) were only administered 1% CMC-Na solution and 20 mice were administered an atherogenic diet to induce hyperlipidemic model. The hyperlipidemic mice were then randomly divided into four groups i.e. positive control mice group (Group 2) were administered simvastatin suspendet in 1% CMC-Na at dose of 0.03 mg/g BW, treatment mice group (Group 3-5) were administered C. hystrix DC leaf extract suspendet in 1% CMC-Na at doses of 0.21; 0.28; and 0.35 mg/g BW, respectively. The interventions were administered one daily for 14 d. Blood sampling from the retroorbital plexus of the mice was conducted before and 14 d after the intervention for lipid profile analysis. The serum total cholesterol, LDL, HDL, and triglyceride levels were measured by the CHOD-PAP (cholesterol oxidase-phenyl amino phyrazolone) enzymatic method. A significant decrease in total cholesterol, LDL, triglycerides levels, and a significant increase in the HDL level were observed in the treatment group at doses of 0.28; 0.35 mg/g BW compared to the normal control group (p<0.05). The maximal antihyperlipidemic activity was observed at dose of 0.28 mg/g BW. In conclusion, the C. hystrix DC leaf extract has antihyperlipidemic model mice.
Uji Aktivitas Antioksidan Esktrak Kasar dan Terpurifikasi Daun Salam (Syzygium polyanthum) Krisna Pertiwi; Dwi Wahyuni; Rosa Juwita Hesturini
JUSTER : Jurnal Sains dan Terapan Vol. 4 No. 2 (2025): JUSTER: Jurnal Sains dan Terapan
Publisher : Jompa Research and Development

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Abstract

Bay leaves (Syzygium polyanthum) are one of Indonesia’s biological resources that have long been utilized, particularly as a cooking spice and in traditional therapy. Current use by the public is still largely based on empirical evidence, and scientific validation is still needed to support its application. The aim of this study was to determine the antioxidant capacity of both crude and purified bay leaf extracts. Extraction was carried out using the maceration method with 96% ethanol as the solvent. Purification of the extract was performed using a liquid-liquid extraction method with a solvent ratio of ethanol to n-hexane = 1:1. Phytochemical screening was conducted on both the crude and purified extracts. Antioxidant activity was evaluated using the DPPH method. Free radical scavenging was measured by UV-Vis spectrophotometry at the maximum wavelength. The results of phytochemical screening indicated that both crude and purified extracts contained alkaloids, flavonoids, tannins, and saponins. The maximum absorbance wavelength was found at λ = 514 nm. The antioxidant activity (IC₅₀) of the crude and purified bay leaf extracts was 15.120 ± 0.039 ppm and 13.293 ± 0.039 ppm, respectively. These results indicate that bay leaves possess strong antioxidant potential.
Co-Authors Ade Giriayu Anjani Dwi Wahyuni KRISNA KHARISMA PERTIWI