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Day, Dafzel
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Chemistry Education Mathematics

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IDENTIFIKASI BAKTERI Edwardsiella ictaluri PADA IKAN PATIN (Pangasionodon hypophthalmus) Manalu, Yemima Clarica; Aprilia, Dina; Anggraini, Heny; Efendi, Syachrani; Day, Dafzel; Astuti, Indri
PendIPA Journal of Science Education Vol 9 No 1 (2025): February
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/pendipa.9.1.204-213

Abstract

Ikan patin (Pangasionodon hypophthalmus) merupakan salah satu jenis ikan yang banyak dibudidayakan dan diproduksi secara massal di Indonesia yang memiliki peluang pengembangan dalam skala industri sebagai ikan konsumsi karena mempunyai potensi pengembangan ekonomi yang tinggi. Namun dalam pengembangannya tidak terlepas dari permasalahan budidaya, salah satunya adanya serangan penyakit Enteric Septicemia of Catfish (ESC) yang disebabkan Edwarsiella ictaluri. Metode penelitian yang digunakan adalah praktik langsung terhadap ikan P. hypophthalmus dan diamati morfologi bakteri secara makroskopis dan mikroskopis, serta biokimiawi sesuai dengan standar uji biokimia. Hasil penelitian menunjukkan bahwa bakteri yang menginfeksi P. hypothlasmus adalah E. ictaluri.
Pemeriksaan TiLV (Tilapia Lake Virus) Pada Ikan Nila (Oreochromis niloticus) Budidaya Fitria, Dina; Fathonah, Deffya; Afrianti, Rini; Turrohmah, Azizah; Mujaidiah; Destiana, Anggari Linda; Day, Dafzel; Astuti, Indri
PendIPA Journal of Science Education Vol 9 No 2 (2025): June
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/pendipa.9.2.310-317

Abstract

Nile Tilapia (Oreochromis niloticus) is highly sought after by Indonesian consumers and is one of the leading commodities cultivated in freshwater. However, tilapia farming still frequently encounters challenges that lead to a decline in production, one of which is infection by pathogens such as Tilapia Lake Virus (TiLV). TiLV can infect fish in waters with temperatures above 25°C, with mass mortality peaking at 30°C, reaching a 50% death rate. Proper handling and control measures are essential to manage TiLV outbreaks effectively. TiLV detection in fish can be conducted using the Polymerase Chain Reaction (PCR) method. PCR-based virus detection is a diagnostic technique that identifies the presence of viral genetic material with high specificity and accuracy. This study aimed to detect TiLV infection in farmed Nile tilapia. The procedures included sample collection, necropsy, organ fixation, RNA extraction, PCR amplification, electrophoresis, and gel documentation analysis. Based on the PCR results, Nile tilapia samples tested positive for TiLV infection, as indicated by the presence of a 250 bp DNA band in the gel documentation system, aligning with the TiLV positive marker. Among the three fish showing suspected TiLV symptoms, two (66.6%) were confirmed infected.
Co-Authors Anggari Linda Destiana Anggraini, Heny Dina Aprilia, Dina Dina Fitria, Dina Efendi, Syachrani Fathonah, Deffya Indri Astuti Manalu, Yemima Clarica Mujaidiah Rini Afrianti Turrohmah, Azizah