Garuda - Garba Rujukan Digital

Nano Liposomal Curcumin as an Adjuvant: Enhancing Cisplatin Anticancer Effects in HeLa Cells Subandi, Subandi; Balindra, Fredlina Rossa; Gizta, Aura Bella; Khotimah, Husnul; Handono, Kusworini
Sciences of Pharmacy Volume 4 Issue 2
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphar0402347

Cervical cancer, mainly driven by oncogenic HPV infections, remains a global health burden. Cisplatin is standard chemotherapy for advanced cases but is limited by toxicity. Nano liposomal curcumin, with improved bioavailability, may enhance cisplatin’s efficacy. This study investigated the combination’s effect on HeLa cells by analyzing Cyclin E1 and Bcl-2 expression. Nano liposomal curcumin was synthesized using thin-film hydration, yielding stable 32 nm nanoparticles. HeLa cells were divided into control and treatment groups, and varying doses of nano liposomal curcumin with cisplatin were received. Flow cytometry revealed significant reductions in Cyclin E1 (from 18.62 ± 1.45 to 5.79 ± 0.47) and Bcl-2 (from 32.56 ± 0.82 to 28.19 ± 0.30) at the highest dose (p < 0.05). Cell viability decreased to 9% with cisplatin alone and 1% with the combination. These results indicate that nano liposomal curcumin enhances cisplatin’s antiproliferative and pro-apoptotic effects, supporting its potential as an adjuvant to lower cisplatin doses while maintaining efficacy. Further research involving additional molecular markers, in vivo models, and clinical trials is needed to optimize dosing, confirm safety, and evaluate therapeutic potential.

A Synergistic Approach: Enhanced Apoptotic and Proliferative Inhibition of Cisplatin by Nanocurcumin in HeLa Cells Subandi, Subandi; Gizta, Aura Bella; Balindra, Fredlina Rossa; Handono, Kusworini; Khotimah, Husnul
Journal of Food and Pharmaceutical Sciences Vol 13, No 2 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.21389

Cisplatin is a widely used chemotherapeutic agent for cervical cancer, but its effectiveness is often limited by cellular resistance and severe side effects. Curcumin has demonstrated potential to enhance cisplatin's anticancer effects by inducing apoptosis and inhibiting cell proliferation; however, its clinical use is constrained by poor solubility and low bioavailability. To overcome these limitations, liposomal nanocurcumin was developed. This study aimed to evaluate the synergistic effects of cisplatin and nanocurcumin on Bax and BrdU expression as markers of apoptosis and proliferation in HeLa cervical cancer cells. A true experimental post-test only design was employed with five groups: HeLa cell control (no treatment), positive control (cisplatin 5 µg/mL), and three treatment groups receiving cisplatin 2.5 µg/mL combined with nanocurcumin at 25, 50, and 100 µg/mL. Cells were incubated for 48 hours and analyzedusing flow cytometry. The combination treatment significantly increased Bax expression and reduced BrdU expression compared to cisplatin alone, with the strongest effect observed at 100 µg/mL nanocurcumin. These findings suggest that nanocurcumin may serve as a promising adjuvant to enhance the therapeutic efficacy of cisplatin by promoting apoptosis and suppressing proliferation in cervical cancer cells.

A Synergistic Approach: Enhanced Apoptotic and Proliferative Inhibition of Cisplatin by Nanocurcumin in HeLa Cells Subandi, Subandi; Gizta, Aura Bella; Balindra, Fredlina Rossa; Handono, Kusworini; Khotimah, Husnul
Journal of Food and Pharmaceutical Sciences Vol 13, No 2 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.21389

Cisplatin is a widely used chemotherapeutic agent for cervical cancer, but its effectiveness is often limited by cellular resistance and severe side effects. Curcumin has demonstrated potential to enhance cisplatin's anticancer effects by inducing apoptosis and inhibiting cell proliferation; however, its clinical use is constrained by poor solubility and low bioavailability. To overcome these limitations, liposomal nanocurcumin was developed. This study aimed to evaluate the synergistic effects of cisplatin and nanocurcumin on Bax and BrdU expression as markers of apoptosis and proliferation in HeLa cervical cancer cells. A true experimental post-test only design was employed with five groups: HeLa cell control (no treatment), positive control (cisplatin 5 µg/mL), and three treatment groups receiving cisplatin 2.5 µg/mL combined with nanocurcumin at 25, 50, and 100 µg/mL. Cells were incubated for 48 hours and analyzedusing flow cytometry. The combination treatment significantly increased Bax expression and reduced BrdU expression compared to cisplatin alone, with the strongest effect observed at 100 µg/mL nanocurcumin. These findings suggest that nanocurcumin may serve as a promising adjuvant to enhance the therapeutic efficacy of cisplatin by promoting apoptosis and suppressing proliferation in cervical cancer cells.

Pengaruh Kombinasi Ekstrak Daun Kelor Dan Sari Kurma Terhadap Peningkatan Kadar Hemoglobin Remaja Putri Sari, Septi Indah Permata; Gizta, Aura Bella; Hindratni, Findy; Sari, Septi; Gizta, Aura
JURNAL PROTEKSI KESEHATAN Vol 14 No 2 (2025): JPK: Jurnal Proteksi Kesehatan
Publisher : Poltekkes Kemenkes Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar

Low hemoglobin levels can cause decreased immunity, healthiness, and thinking dexterity which leads to reducing learning achievement and productivity of adolescent girls. There were 21,010 women of reproductive age in Indonesia that suffering from low hemoglobin levels in 2019. The purpose of this study was to determine the effect of the combination of moringa leaf extract and date palm extract on increasing hemoglobin levels of adolescent girls at MAN Tanjungpinang. This research was conducted in January – April 2022, with samples of 15 adolescent girls. Samples were given intervention with 2x500 mg moringa leaf extract and 2x15 ml date palm extract for 12 days. Data analyzed using Wilcoxon test with 5% α. The results showed that the average hemoglobin level before intervention was 14,147 g/dL, and the average hemoglobin level after intervention was 15,960 g/dL. There was an effect of the combination of moringa leaf extract and date palm extract on increasing hemoglobin levels of adolescent girls at MAN Tanjungpinang (p = 0,001).

SPEECH DELAY IN CHILDREN: UNRAVELING THE ROLES OF FRAGMENTED FAMILY, INADEQUATE CAREGIVER, AND EXCESSIVE PHONE USAGE – CASE REPORT Gizta, Aura Bella; Sururi, Desy Aulina; Wahyuningtyas, Mariana; Arsini, Putu Sudewi; Putri, Winarni Dian Dwiastuti Wisnu
Journal of Psychiatry Psychology and Behavioral Research Vol. 7 No. 1 (2026): Multidimensional Perspectives on Mental Health Across the Lifespan and Social C
Publisher : Departemen Psikiatri Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jppbr.2026.007.01.10

Introduction: This article discusses the risk factors for speech delay, a communication development disorder, specifically the development of speech and language in children. Communication disorders can disrupt motor and cognitive development. Risk factor detection is necessary to prevent speech delay and ensure that the treatment is suitable. Methods: This study employed a qualitative descriptive research design using a case study approach. Data collection techniques included observation, interviews, and documentation. The case study involved one subject, a 47-month-old male child. Qualitative data analysis techniques were conducted using the Miles and Huberman model. Results: Signs and symptoms of speech delay in N were already shown at the age of 24 months, and the definitive diagnosis was made at the age of 32 months. More than one non-medical risk factor was found, including a fragmented family, an inadequate caregiver, and excessive phone usage. The therapy provided was a combination of speech therapy with visual aids, actively reading stories from books, and fulfilling social interaction needs. Discuss: Child development milestones should always be monitored to identify signs and symptoms of speech delay. Kuesioner Pra-Skrining Perkembangan [KPSP] screening can be used for early detection of child development disorders. The therapy implemented should be followed up to ensure progress in speech and language development. Conclusion: N's speech delay is caused by a fragmented family, inadequate caregiver, and excessive phone usage. We provide support to parents/caregivers. We also recommend following up on the monitoring of N’s speech and language development progress. Keywords: speech delay, communication, phone usage.

Title

Found 5 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 5 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 5 Documents
Search