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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Legal: Journal of Law
Sudarwin
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Health Professions Law, Crime, Criminology & Criminal Justice Social Sciences

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Exploration and molecular identification of proteolytic bacteria from rusip pacific oyster (Crassostrea gigas) as anticoagulant agent candidates Afriansyah, Muhammad Ardi; Sudarwin; Dewi, Sri Sinto; Akbar, Gusti Dimas Refian
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6083

Abstract

The marine symbiont Staphylococcus epidermidis strain CGF-6, a protease-producing bacterium, has been successfully isolated from Rusip Pacific Oyster (Crassostrea gigas). S. epidermidis is a non-spore-forming, Gram-positive coccus commonly found in marine environments due to their ability to tolerate high salinity. The aim of this study was to identify proteolytic bacteria from Rusip fermented C. gigas as potential candidates for the development of anticoagulant agents. Bacterial isolation was performed through the fermentation process of Rusip. After seven days, bacterial colonies were purified three times using Nutrient Agar. The selection of proteolytic bacterial was conducted qualitatively using a skim milk agar medium. The bacterial isolates exhibiting the highest protease activity were identified through 16S rRNA gene sequencing using universal primers Bact 27F and UniB 1492R. Phylogenetic tree analysis, conducted with the MEGA X program, helped determine the relationships between species. Out of the 18 bacterial isolates obtained from the Rusip fermentation of C. gigas, three isolates (CGF-1, CGF-6, and CGF-11) exhibited hydrolysis zones around their colonies on skim milk agar, indicating protease activity.  Among these, isolate CGF-6 showed the highest proteolytic index of 0.5 and was identified as Staphylococcus epidermidis strain CGF-6. S. epidermidis strain CGF-6 has the potential to serve as a valuable source of protease production for the development of anticoagulant agents. However, further studies, including enzyme characterisation, optimisation, and both in vitro and in vivo anticoagulant activity tests, are necessary to assess the efficacy and safety of this enzyme as a candidate for anticoagulant agents.
Tinjauan Psikologi Hukum Terhadap Kematangan Emosi, Kontrol Diri dan Perilaku Agresif pada Anggota Polri dalam Menangani Huru Hara Nur, Andi Wahyuddin; Yustiana; Hendra Sudrajat; Baso Muhammad Harisah; Andi Astrid Baradillah; Sudarwin
Legal Journal of Law Vol 4 No 2 (2025): Edisi: November 2025
Publisher : YP-SDI Lamaddukelleng

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Abstract

This study aims to determine the emotional maturity, self-control, and aggressive behavior of Polri members in handling riots; and to determine the factors that influence emotions, self-control, and aggressive behavior of Polri members in handling riots. The study was conducted at the Wajo Police. Data collection methods used were interviews and library research. Data were analyzed qualitatively using deductive and inductive methods and then presented descriptively. Data in the form of direct interviews with related parties and in the form of legal products and reading materials related to the object of study to sharpen and deepen the qualitative analysis. The results of the study indicate that emotional maturity and self-control are important factors in shaping the behavior of Polri members who are professional and have integrity, especially when facing riot situations. By improving these two aspects, it is hoped that Polri members can carry out their duties more effectively, maintain security and order, and minimize the negative impact of aggressive behavior; and The factors that influence emotions, self-control, and aggressive behavior of Polri members in handling riots are very complex and diverse. Some internal factors include age, temperament, emotional maturity, and self-esteem. External factors include past experiences, social support, the work environment, and task pressure. Furthermore, conditions such as sleep deprivation, stress, and a lack of understanding of the task can also trigger emotional instability and aggressive behavior
Co-Authors Afriansyah, Muhammad Ardi Akbar, Gusti Dimas Refian Andi Astrid Baradillah Baso Muhammad Harisah Hendra Sudrajat Nur, Andi Wahyuddin Sri Sinto Dewi Yustiana