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All Journal JBIO: jurnal biosains (the journal of biosciences) Best Journal (Biology Education, Sains and Technology)
Syarifah Riska Mela Putri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Public Health

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PHYSICOCHEMICAL CHARACTERISTICS OF ECO-ENZYME DERIVED FROM DURIAN ALBEDO AS A SOLUTION FOR WASTE MANAGEMENT Harahap, Doli Fadly; Ayu, Gabriella Marry; Putri, Syarifah Riska Mela
JURNAL BIOSAINS Vol. 10 No. 3 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i3.63717

Abstract

North Sumatra is the largest durian-producing province in Indonesia. As a result, durian skin waste has increased, reaching 332,712 tons annually. Durian skin is divided into the outer layer, known as the flavedo and the inner layer called the albedo. Durian skin contains chemical compounds such as lignin and tannins, which hinder its efficient decomposition. Therefore, environmentally friendly solutions for processing durian skin waste are needed. One such solution is the production of eco-enzyme. Eco-enzyme is a complex solution produced by fermenting organic waste with molasses and water, using selective microorganisms. It has various applications, such as household cleaners, organic fertilizers, and pest control. The purpose of this study was to identify the physicochemical characteristics and quality of the eco-enzyme produced, as well as its potential to reduce environmental pollution. The research employed a descriptive qualitative method with an experimental approach. The process involved coarsely chopping durian albedo and mixing it with molasses and water in a 1:3:10 ratio (sugar, albedo, and water). The mixture was fermented for three months, sealed tightly in a container, and periodically opened. The resulting liquid was the eco-enzyme. Physicochemical tests were conducted to evaluate pH, aroma, color, total dissolved solids (TDS) and other properties, including Wagner's Reagent test, Alkaline Reagent test, Foam test, and Quinone test. The results showed that durian albedo is highly suitable for producing eco-enzyme, yielding a product with a pH of 2.4, a TDS value of 855, a blackish-brown color, a fruity fermentation aroma, and containing phytochemical compounds such as alkaloids, flavonoids, saponins, and quinones.
Effectiveness of Okra (Abelmoschus esculentus (L.) Moench) as an Ethnomedicine Based Anticancer Agent through Teratogenic Risk Testing Syarifah Riska Mela Putri; Rahmi
JURNAL BIOSAINS Vol. 12 No. 1 (2026): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/ca2cfa79

Abstract

Okra (Abelmoschus esculentus L.) contains lectin compounds known for their potential anticancer properties. However, limited research has examined its possible teratogenic effects—adverse impacts that may cause fetal abnormalities. The urgency of this study lies in assessing the safety of okra extract as a natural anticancer candidate. This research aimed to evaluate the teratogenic effects of okra fruit extract using an in vivo model as a preliminary step toward developing safe ethnomedicine-based anticancer agents. The study employed a Completely Randomized Design (CRD) with one control and three treatment groups. Female mice (Mus musculus L., DDW strain) received 2%, 4%, and 6% ethanol extracts of okra orally at a dose of 0.01 mL/g body weight daily from gestation days 0–10. The control group received no extract. Observations included maternal and fetal body weights, number of fetuses, liver morphology, and congenital malformations such as cleft palate and hydrocephalus. Data were analyzed using ANOVA (p < 0.05) followed by Bonferroni Post Hoc tests in SPSS version 23. The results showed that okra extract significantly affected maternal weight, fetal number, and fetal weight, as well as liver morphology and cleft palate incidence. These findings indicate that although okra exhibits anticancer potential, its teratogenic risks must be carefully evaluated.
Physical Characterization of Bioplastics Made from Sweet Orange (Citrus sinensis) Peel Waste Pectin and Pandan Leaf (Pandanus amaryllifolius) Cellulose Gabriella Marry Ayu; Nikmatul Khoiriah Harahap; Syarifah Riska Mela Putri
JURNAL BIOSAINS Vol. 12 No. 1 (2026): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/nrt8fk52

Abstract

One effort to reduce environmental pollution is developing bioplastics from organic waste, such as pectin from sweet orange peel (Citrus sinensis) and cellulose from fragrant pandan leaves (Pandanus amaryllifolius). Pectin, a polysaccharide from orange peel, and cellulose from pandan leaves can produce biodegradable plastics easily decomposed by soil microorganisms. This study addresses the growing issue of plastic waste accumulation by creating eco-friendly packaging alternatives. Utilizing orange peel and pandan leaves aligns with the circular economy concept by turning food waste into valuable materials. The research aims to reduce plastic pollution and process organic waste into bioplastics based on pectin and cellulose. An experimental laboratory design using a Completely Randomized Design (CRD) was applied with pectin concentrations of 1 g, 2 g, and 3 g, each repeated three times. The formulation also included 1 g of pandan cellulose, carrageenan (0.8 g) as a stabilizer, and glycerol (2%) as a plasticizer. Data were analyzed using one-way ANOVA in SPSS 24. Results showed that increasing pectin concentration enhanced bioplastic thickness, reduced water absorption, and accelerated degradation over four weeks, indicating improved biodegradable performance.
The Effect of Okra Powder Fortification on the Proximate Composition and Glycemic Index of Rice as a Low-Sugar Staple Food for Individuals with Diabetes Nindya Anggiani Sembiring; Doli Fadly Harahap; Syarifah Riska Mela Putri
JURNAL BIOSAINS Vol. 12 No. 1 (2026): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/eaav2050

Abstract

The number of individuals with diabetes in Indonesia continues to rise, largely due to the habitual consumption of rice with a high glycemic index that triggers blood glucose spikes. Since rice is an essential part of the Indonesian diet, a practical solution is needed to lower its glycemic impact. This study aimed to analyze the effect of okra powder fortification on the proximate composition and glycemic index of rice as a low-sugar staple food for individuals with diabetes. The research was conducted experimentally using a Completely Randomized Design (CRD) with 50 respondents. Treatments included two controls (fasting and rice without okra) and three fortification levels: 5 g, 10 g, and 15 g of okra powder per 100 g of rice. Proximate analysis covered moisture, ash, protein, fat, and carbohydrate content, while sensory evaluation used a hedonic test. Blood glucose levels were measured at 0, 30, 60, 90, and 120 minutes after consumption, and data were analyzed using ANOVA and Duncan’s Multiple Range Test (DMRT). Results showed that rice fortified with 15 g of okra powder had higher viscosity, reduced glucose absorption, and a lower glycemic index. The product was well accepted in taste, though texture refinement is still needed.
UJI EFEK NEFROPROTEKTIF EKSTRAK ETANOL DAUN BIWA (Eriobotrya japonica Lindl) TERHADAP KADAR KREATININ TIKUS MODEL KANKER PAYUDARA YANG DIINDUKSI Benzo(a)Pyrene Syahfitri, Widya; Elizabeth, Fitri; Putri, Syarifah Riska Mela; Maelani, Imelda; Hutahaean, Salomo; Ilyas, Syarifuddin
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.11120

Abstract

Benzo(a)pyrene (BaP) merupakan senyawa toksik dan karsinogenik yang dapat menyebabkan stres oksidatif, peradangan, dan gangguan fungsi organ, termasuk ginjal. Penelitian ini bertujuan untuk menguji efek nefroprotektif ekstrak metanol daun biwa (Eriobotrya japonica) terhadap kadar kreatinin tikus model kanker payudara yang diinduksi BaP. Penelitian ini menggunakan rancangan acak lengkap dengan lima kelompok perlakuan, yaitu kontrol negatif (tanpa perlakuan), kontrol positif (BaP 50 mg/kg BB), serta tiga kelompok perlakuan dengan pemberian ekstrak daun biwa dosis 200, 300, dan 400 mg/kg BB selama 30 hari. Kadar kreatinin diukur menggunakan metode modifikasi Jaffe. Data hasil penelitian dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa kadar kreatinin serum darah tikus pada semua kelompok masih berada dalam rentang normal, dengan nilai antara 0,66 hingga 0,73 mg/dl, dan tidak terdapat perbedaan yang signifikan (p0,05) antara kelompok perlakuan dan kontrol. Hal ini mengindikasikan bahwa paparan BaP tidak secara langsung mengganggu fungsi ginjal tikus model kanker payudara, serta pemberian ekstrak daun biwa tidak menunjukkan efek protektif yang signifikan terhadap kadar kreatinin. Namun, kandungan antioksidan dalam daun biwa, seperti flavonoid dan triterpenoid, tetap berpotensi dalam menjaga stabilitas fungsi ginjal melalui mekanisme antioksidatif dan antiinflamasi
Co-Authors Ayu, Gabriella Marry Doli Fadly Harahap Elizabeth, Fitri Gabriella Marry Ayu Harahap, Doli Fadly Ilyas, Syarifuddin Maelani, Imelda Nikmatul Khoiriah Harahap Nindya Anggiani Sembiring Rahmi Salomo Hutahaean Syahfitri, Widya