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All Journal JBIO: jurnal biosains (the journal of biosciences) Saintia Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Prosiding Seminar Biologi Best Journal (Biology Education, Sains and Technology) IJOEP : International Journal of Ecophysiology
Salomo Hutahaean
Departemen Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Public Health

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Kerentanan Palatogenesis Mencit (Mus musculus L.) terhadap Induksi Cleft Palate TCDD Hutahaean , Salomo; Mangkoewidjojo , Soesanto; Sagi , Mammed; Asmara , Widya
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.091 KB) | DOI: 10.24002/biota.v16i2.100

Abstract

Telah dilakukan percobaan untuk menentukan tahapan palatogenesis pada mencit (Mus musculus L.) yang rentan terhadap efek polutan 2,3,7,8-Tetraklorodibenzo-p-dioksin (TCDD). Percobaan dirancang mengikuti Rancangan Acak Lengkap dengan pola faktorial (4X3). Empat puluh delapan ekor mencit bunting dicekok TCDD dengan dosis 0 (kontrol), 5, 10, atau 20 μg/kg bb. Perlakuan diberikan pada hari kebuntingan (Hk) 9−10, 11−12, atau 13−14. Mencit kontrol dicekok pelarut saja (98,5% minyak wijen + 1,5% DMSO). Pada Hk 18 mencit dibius lalu dibunuh dengan teknik cervical dislocation, persentase fetus cleft palate (cp) dihitung, derajat penutupan palatum diberi skor, preparat dengan ketebalan 6 µm dibuat, dan mikrostruktur kraniofasial diamati. Hasil menunjukkan, pemberian TCDD antara hari ke 9 dan 12 menginduksi cacat cp, dengan kecenderungan hasil tertinggi pada pemberian Hk 910. Perlakuan TCDD dosis 10 atau 20 μg/kg bb pada Hk 910 menghasilkan fetus cacat cp >90%. Persentase fetus cp tetap tinggi pada pemberian Hk 1112, khususnya pada kelompok dosis 20 μg/kg bb (87,3%). TCDD dosis terendah (5 μg/kg bb) menginduksi cp dominan bercelah sempit, menunjukkan adanya hambatan pada tahap fusi. Dosis 10 dan 20 μg/kg bb menginduksi cp bercelah sedang atau lebar, mengisyaratkan terjadi hambatan pada tahap inisiasi atau elevasi. Disimpulkan, seluruh tahapan palatogenesis rentan terhadap efek TCDD, namun tahap paling rentan adalah tahap fusi palatum.
Pengaruh Ekstrak Methanol Biji Pare (Momordica charantia) dan DMPA Terhadap Jumlah Sel purkinje Cerebellum Mencit (Mus musculus L.) SYAFRUDDIN ILYAS; SALOMO HUTAHAEAN; NURSAL NURSAL
Prosiding Seminar Biologi Vol 2 No 1 (2016): Prosiding Seminar Nasional From Basic Science to Comprehensive Education
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v2i1.3379

Abstract

Perubahan jumlah sel purkinje cerebellum mencit dapat dijadikan sebagai indikator terjadinya gangguan terhadap otak kecil pada pemberian ekstrak methanol biji pare (Momordica carantia L.) dan DMPA. Metode eksperimen digunakan untuk menentukan perbedaan yang terjadi pada tiap kelompok kontrol dan pemberian lama pemberian ekstrak biji pare dan DMPA. Kelompok kontrol terbagi dalam K0, K1, dan K2 dengan waktu berturut-turut 0, 4, dan 8 minggu. Kelompok perlakuan terdiri dari (P0) biji pare 0 minggu secara oral dan DMPA intramuskular (@ 6 jam), (P1) biji pare dan DMPA (@4 minggu), (P2) biji pare dan DMPA (@8 minggu). Masing-masing kelompok terdiri dari 5 mencit sehingga total mencit jantan adalah 30 ekor. Dosis ekstrak metanol biji pare adalah 5mg/10g berat badan mencit yang diberikan secara oral) (Yama et al. 2011). Sedangkan dosis DMPA sebesar 0,175 mg/ekor mencit yang diberikan secara intramuskular (Ilyas 2014). Hasil penelitian menunjukkan tidak adanya perbedaan yang nyata  (p>0,05) antara kontrol dan perlakuan pada 0, 4, dan 8 minggu terhadap jumlah sel purkinje cerebellum mencit. Disimpulkan bahwa pemberian ekstrak metanol biji pare dan DMPA aman terhadap perbedaan jumlah cerebellum mencit. Kata kunci: Sel purkinje, Momordica charantia L., DMPA, Mus musculus L. 
Kerentanan Palatogenesis Mencit (Mus musculus L.) terhadap Induksi Cleft Palate TCDD Salomo Hutahaean; Soesanto Mangkoewidjojo; Mammed Sagi; Widya Asmara
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.100

Abstract

Telah dilakukan percobaan untuk menentukan tahapan palatogenesis pada mencit (Mus musculus L.) yang rentan terhadap efek polutan 2,3,7,8-Tetraklorodibenzo-p-dioksin (TCDD). Percobaan dirancang mengikuti Rancangan Acak Lengkap dengan pola faktorial (4X3). Empat puluh delapan ekor mencit bunting dicekok TCDD dengan dosis 0 (kontrol), 5, 10, atau 20 μg/kg bb. Perlakuan diberikan pada hari kebuntingan (Hk) 9−10, 11−12, atau 13−14. Mencit kontrol dicekok pelarut saja (98,5% minyak wijen + 1,5% DMSO). Pada Hk 18 mencit dibius lalu dibunuh dengan teknik cervical dislocation, persentase fetus cleft palate (cp) dihitung, derajat penutupan palatum diberi skor, preparat dengan ketebalan 6 µm dibuat, dan mikrostruktur kraniofasial diamati. Hasil menunjukkan, pemberian TCDD antara hari ke 9 dan 12 menginduksi cacat cp, dengan kecenderungan hasil tertinggi pada pemberian Hk 910. Perlakuan TCDD dosis 10 atau 20 μg/kg bb pada Hk 910 menghasilkan fetus cacat cp >90%. Persentase fetus cp tetap tinggi pada pemberian Hk 1112, khususnya pada kelompok dosis 20 μg/kg bb (87,3%). TCDD dosis terendah (5 μg/kg bb) menginduksi cp dominan bercelah sempit, menunjukkan adanya hambatan pada tahap fusi. Dosis 10 dan 20 μg/kg bb menginduksi cp bercelah sedang atau lebar, mengisyaratkan terjadi hambatan pada tahap inisiasi atau elevasi. Disimpulkan, seluruh tahapan palatogenesis rentan terhadap efek TCDD, namun tahap paling rentan adalah tahap fusi palatum.
Test Antihyperglycemia Ethanol Leaf Extract Kembang Bulan ( Tithonia diversifolia) in Mice ( Mus musculus ) The Induced Diabetes With alloxan Ronald Pasaribu; Salomo Hutahaean; Syafruddin Ilyas
JBIO: jurnal biosains (the journal of biosciences) Vol 1, No 2 (2015): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v1i2.2785

Abstract

A research was conducted to invenstigate antihyperglycemic activity ethanol extract of leaf kembang bulan (Tithonia diversifolia) in mice with alloxan induced diabetes. The experiment used completely randomized design, 6 treatment groups with 5 replicates each. KP mice, P1, P2, P3 and P4 fasted for 16 hours and then injected intraperitoneally alloxan 5.04 mg. On day 4 post induction, KP, P1, P2, P3 and P4 positive diabetes. P1, P2 and P3 were treated 14; 42 and 70 mg/Kg of ethanol extract of kembang bulan while P4 were 0.9 mg/Kg glibenclamide for 12 days. The results showed that all treatment doses did not significantly affect body weight of diabetic mice (P>0.05). P1 obtained with a dose of 14 mg/Kg body weight is able to decrease blood glucose levels (P<0,05). The ethanol extract at a dose of 14 mg/Kg body weight effect on decreasing the number of giant cells of the spleen. It can be concluded ethanol extract of kembang bulan can lower blood glucose levels  and effect  the  microstructure spleen of mice  with alloxan induced diabetes. Keywords : Alloxan , Ethanol Extract , Tithonia diversifolia , Blood Glucose Levels.
Kualitas Spermatozoa Sapi Limousin Dalam Pengencer Two-Stetm Extender Dengan Suplementasi Kuning Telur Bebek Selama Penyimpanan Pada Refrigerator Ibezaro Zega; Syafruddin Ilyas; Salomo Hutahaean
JBIO: jurnal biosains (the journal of biosciences) Vol 1, No 3 (2015): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v1i3.2925

Abstract

Tujuan dari Penelitian ini adalah mengetahui pengaruh penambahan kuning telur bebek dalam pengencer two-steptm extender terhadap motilitas, viabilitas dan morfologi spermatozoa sapi limousin. Penelitian menggunakan eksperimen laboratorium menggunakan pola rangcangan acak lengkap (RAL) dengan 5 kali ulangan dari 5 ejakulasi dari sapi yang sama. Spermatozoa disimpan selama sepuluh hari. Motilitas spermatozoa diamati dengan mikroskop cahaya (400x) pada suhu 37°C. Viabiltas spermatozoa diamati dengan pewarnaan eosin menggunakan mikroskop cahaya (400x), morfologi spermatozoa diamati dengan pewarnaan metilen blue menggunakan mikroskop cahaya (400x). Hasil penelitian menunjukkan suplementasi kuning telur bebek pada pengencer two-steptm extender berpengaruh terhadap motilitas dan viabilitas, morfologi spermatozoa sapi limousin selama penyimpanan pada suhu 4-5°C. Konsentrasi kuning telur terbaik adalah 15% dalam mempertahankan motilitas (41,00%±2,24), viabilitas (74,44±4,77%) dan morfologi (69,91±1,98%) spermatozoa sapi Limousin setelah penyimpanan empat hari pada suhu 4-5°c.   Kata kunci: two-steptm extender, kuning telur, motilitas, viabilitas, morfologi spermatozoa sapi Limousin .
KELAINAN PERKEMBANGAN KRANIOFACIAL FETUS MENCIT (Mus musculus L.) Strain DDW SETELAH PEMBERIAN EKSTRAK N-HEKSAN BUAH ANDALIMAN (Zanthoxylum acanthopodium DC.) Mai Sarah; Emita Sabri; Salomo Hutahaean
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.894 KB)

Abstract

The purpose of this study is  to investigate the effect of n-hexane extract of andaliman (Zanthoxylum acanthopodium DC.) fruit on craniofacial development of DDW Mice (Mus musculus L.). Experiment was carried out using Completely Randomized Design (CRD) with 5 treatments and 6 replicates. Treatments  consist  of a blank control, vehicle control (1% CMC) and 3 concentrations of andaliman extract (2%, 4% and 6%). All treatments were administered by oral gavage (0.1 ml/10 g bw) once  daily from day 0 to day 10 of gestation.  N-hexane extract of andaliman fruit induced developmental abnormalities in craniofacial structures i.e. increased the incidence of microphthalmia (P <0.05), and hydrocephalus (P <0.05). In conclusion, we suggested that andaliman fruit may have fetotoxic effects.   Keyword: andaliman (Zanthoxylum acanthopodium DC.), micropthalmia, hydrocephalus
GAMBARAN HISTOLOGIS PULMO MENCIT JANTAN (Mus musculus L.) SETELAH DIPAPARI ASAP ROKOK ELEKTRIK Nanin Triana; Syafruddin Ilyas; Salomo Hutahaean
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.2 KB)

Abstract

The effects of electric cigarette exposure on lung tissue of mice were studied. The experiments using completely randomized design (CRD) with 3 treatments, i.e.  treatment of electric cigarette with strawberry flavour, tobacco flavour, and control treatment that was not exposed by cigarette smoke. Eight male mice were used for each group. Exposure treatment was done every day for 2 consecutive weeks with dosage 20 times suction using syringe of 60 mL. After receiving treatment for 2 weeks, the mice were killed by cervical dislocation. The lungs were isolated and prepared histologically using paraffin method and then stained with by haematoxylin and eosin  (HE). The results showed that, smoke treatment mice lose weight significantly (p< 0.05). In gross anatomy, electric cigarrete smoke affect lung feature, but histological observation on  membrane damage, damage to the lumen and the relationship between alveoli the smoke effect were statistically unsignificant (p>0.05).. In summary short period exposure to electric  cigarette smoke  tends affect lung damage in anatomy, but statistically has no effect of the damage to the lung tissue.   Keywords: alveolar lumen, alveolar membrane, electric cigarettes
PENGARUH PEMBERIAN VITAMIN C DAN E TERHADAP GAMBARAN HISTOLOGIS HEPAR MENCIT (Mus musculus L.) YANG DIPAJANKAN MONOSODIUM GLUTAMAT (MSG) Ahri Maulida; Syafruddin Ilyas; Salomo Hutahaean
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.043 KB)

Abstract

This study aimed to determine the effects of vitamin C and E on liver microstructure of mouse exposed to monosodium glutamate (MSG) for 30 days. The experiment used completely randomized design which consists of 6 treatments and  5 replications. Mice were treated daily by oral gavage with 4 mg MSG (P1),  4 mg MSG + 0.26 mg  vitamin C (P2),  4 mg MSG + 0.026 vitamin E (P3), or  4 mg  MSG + 0.26 mg  vitamin C + 0.026 mg vitamin E (P4). All dosages were based on mg/g body weight. Two control groups K- and K+ were treated with water and castor oil respectively. The mice liver tissue slide was prepared by the paraffin method and stained with haematoxylin-eosin (HE). The results showed that MSG caused cell degeneration and necrosis in liver tissue. Vitamin C and E was  able to recover  liver cell damage induced by MSG.  The highest result was found in P3 and combination treatment (P4) where the recovery effect reaches 64% and 80% respectively. Keywords : Liver, Monosodium glutamate (MSG), Vitamin C, Vitamin E.
PENGARUH PEMBERIAN VITAMIN C DAN E TERHADAP GAMBARAN HISTOLOGIS GINJAL MENCIT (Mus musculus L.) YANG DIPAJANKAN MONOSODIUM GLUTAMAT (MSG) Zulfiani Zulfi; Syafruddin Ilyas; Salomo Hutahaean
Saintia Biologi Vol 1, No 3 (2013)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.862 KB)

Abstract

The aim of this research is to study the effect of vitamin C and E on kidney microstructure of mouse exposed to monosodium glutamate for 30 days. This research used complete randomized design which consisted of 6 treatments and 5 replications. The control consisted of  K-, and K+ (castor oil 0.3 ml). The treatments were MSG 4 mg/g BW, P1 (only MSG), P2 (MSG plus vitamin C 0.26 mg/g BW), P3 (MSG plus vitamin E 0.026 mg/g BW) and P4 (MSG plus vitamin C and E). After treatments were completed, the mice were sacrificed by cervical dislocation, and kidney tissue section was prepared by  the paraffin method and  stained with Haematoxylin and Eosin staining. Microstructure damage of kidneys showed a significant difference between control and treatments. In P1, the proximal tubule damage was 69.95%±5 and in P4 was 47.22%±3. In summary MSG can cause damage in the form of a narrowing of the lumen of the proximal tubules of the kidney. A combination of vitamins C and E can reduce the effect of MSG on the kidney.   Keywords: kidney, Monosodium glutamat (MSG), Vit C, Vit E.
PENGARUH PEMBERIAN VITAMIN C DAN E TERHADAP GAMBARAN HISTOLOGIS TESTIS MENCIT (Mus musculus L.) YANG DIPAJANKAN MONOSODIUM GLUTAMAT (MSG) Ummi Kalsum; Syafruddin Ilyas; Salomo Hutahaean
Saintia Biologi Vol 1, No 3 (2013)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.802 KB)

Abstract

The aim of this study is to determine the effect of vitamin C and E on  testicular histology of mice following exposure to monosodium glutamate (MSG) for 30 days. Completely Randomized Design (CRD) consisting of 6 treatments and 5 replications was used in this experiment. Mice were treated daily by oral gavage with 4 mg MSG (P1),  4 mg MSG + 0,26 mg  vitamin C (P2),  4 mg MSG + 0,026 vitamin E (P3), and  4 mg  MSG + 0,26 mg  vitamin C + 0,026 mg vitamin E (P4). All dosages (mg/g) were given based on body weight. Two control groups K- and K+ treated with water and castor oil respectively. The results showed that MSG decrease testis weight and its volume (P<0,05), MSG also decrease the diameter of seminiferous tubule and the number of spermatogenic cells. Vitamin C recovers testis weight (P<0,05), but cannot recover its volume, diameter of seminiferous tubules and the number  spermatogenic cells (P>0,05). Vitamin E increases the weight and volume of the testes and spermatogenic cells (P<0,05), but unable to recover diameter of the seminiferous tubules (P>0,05). The combination of vitamin C and E recover testis weight and its volume, seminiferous  tubule  diameter  and number  of  spermatogenic  cells (P<0,05). Keywords: Monosodium Glutamate, Vitamin C, Vitamin E, Testis
Co-Authors Ahri Maulida Elizabeth, Fitri Emita Sabri Ibezaro Zega Ilyas, Syarifuddin Maelani, Imelda Mai Sarah Mammed Sagi Mangkoewidjojo , Soesanto Nanin Triana Nursal Putri, Syarifah Riska Mela Ronald Pasaribu Sagi , Mammed Soesanto Mangkoewidjojo Syafruddin Ilyas Syafruddin Ilyas Syahfitri, Widya Ummi Kalsum Widya Asmara Zulfiani Zulfi