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All Journal JBIO: jurnal biosains (the journal of biosciences) Best Journal (Biology Education, Sains and Technology) Narra J Jurnal Natural
Maelani, Imelda
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Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Energy Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Physics Public Health

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Comparative study of anti-SARS-CoV-2 receptor-binding domain total antibody titer before and after heterologous booster with mRNA-based COVID-19 vaccine Kamil, Qatrunnada; Putri, Widia; Ayulinda, Arianisah P.; Maelani, Imelda; Anwar, Samsul; Ichsan, Ichsan; Pranata, Agung; Mudatsir, Mudatsir; Syukri, Maimun; Rizal, Samsul; Kurniawan, Rudi; Sofyan, Sarwo E.; Harapan, Harapan
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.788

Abstract

The waning immunity following the COVID-19 vaccination become a significant concern and the immunological dynamics of vaccine-induced antibodies after vaccination need to be explored. The aim of this study was to compare anti-SARS-CoV-2 receptor-binding domain (RBD) antibody levels before and after a booster dose with heterologous COVID-19 vaccine and to identify factors influencing the levels after receiving the booster dose. A cross-sectional study was conducted in which individuals who received primary doses of CoronaVac and a booster dose with an mRNA-based vaccine were recruited using a purposive sampling technique. The titers of anti-SARS-CoV-2 RBD antibodies were measured using an enzyme-linked immunosorbent assay (ELISA), and plausible associated factors were collected using a questionnaire-assisted face-to-face interview. The Wilcoxon test was used to compare the titers before and after the booster dose, while the Kruskal-Wallis and Mann-Whitney tests, followed by multivariate linear regression, were used to assess the factors associated with RBD total antibody titers. The results showed that there was a significant increase of anti-SARS-CoV-2 RBD total antibody titers before and after receiving the booster dose (1,558.7 BAU/mL vs 140.6 BAU/mL, p<0.001). The analysis revealed that age (p=0.555), sex (p=0.254), type of vaccine (p=0.914), presence of hypertension (p=0.541), diabetes (p=0.975), chronic obstructive pulmonary disease (COPD, p=0.620), and gout (p=0.364) were not associated with anti-SARS-CoV-2 RBD total antibody titers. However, the titers of anti-SARS-CoV-2 RBD total antibody were significantly different between those with and without hyperlipidemia (p=0.021). This study suggests that a booster dose with a heterologous COVID-19 vaccine could significantly enhance immune responses against COVID-19, and therefore, this strategy may be recommended as part of preventive measures to strengthen immunity against COVID-19.
Acceptance for a booster dose of COVID-19 vaccine in Indonesia: A follow-up study Harapan, Harapan; Maelani, Imelda; Anwar, Samsul; Latief, Kamaluddin; Mellinia, Sania A.; Nanda, Cut M.; Nainu, Firzan; Nirwana, Aura; Aksa, Rahmad; Sarifuddin, Sarifuddin; Astri, Yesi; Fathima, Raisha; Nalapraya, Widhy Y.; Ikram, Ikram; Mutiara, Suci; Syahraini, Aigia; Mudatsir, Mudatsir
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.803

Abstract

The coronavirus disease 2019 (COVID-19) vaccination program in Indonesia has been implemented as a key strategy to mitigate the spread of the virus within communities. The success of this program depends on public acceptance of COVID-19 vaccines, including booster doses. The aim of this study was to assess the acceptance of the COVID-19 booster dose in Indonesia and to identify factors influencing individuals' acceptance. A cross-sectional study was conducted across 34 provinces in Indonesia on June 2023. Logistic regression analysis was used to identify the factors associated with booster dose uptake. The findings revealed that 88.8% (2,049/2,308) of respondents were willing to receive a booster dose if provided free of charge by the Indonesian government. However, acceptance decreased to 61.7% when respondents were informed of a 20% likelihood of side effects, even with a reported 95% vaccine efficacy. Adjusted logistic regression analysis identified ten significant factors associated with booster dose acceptance: sex, age, religion, history of previous COVID-19 infection, type of primary vaccine received, belief in vaccine-related conspiracy theories, trust in traditional medicine conspiracies, confidence in natural immunity, perceived vaccine efficacy, and perceived vaccine effectiveness. These findings suggest that acceptance of COVID-19 booster doses in Indonesia is influenced by intrinsic and extrinsic factors, including limited knowledge of booster dose benefits and concerns about potential side effects. To enhance public acceptance, targeted health campaigns and educational initiatives should be intensified, emphasizing the safety, efficacy, and importance of booster vaccinations in controlling the COVID-19 pandemic.
Bacterial patterns and antibiotic susceptibility among pus culture in dr. Zainoel Abidin General Hospital, Banda Aceh KEUMALAHAYATI, MEUTIA; HAYATI, ZINATUL; MAELANI, IMELDA; RIDWAN, AZWAR; RAHMAWATI, CUT VIKA; MULIANA, DESI; PUTRI, FARAH DHITA ANISSA; MAHDANI, WILDA
Jurnal Natural Volume 25 Number 1, February 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i1.44523

Abstract

The collection of pus samples is crucial for identifying serious bacterial infections. Accurate identification of the pathogen and selection of the appropriate antibiotics are essential for effective treatment. This study used a descriptive retrospective method to investigate pus specimens bacterial patterns and antibiotic susceptibility at dr. Zainoel Abidin General Hospital in Banda Aceh in 2023. Out of 1,274 examined pus specimens, 782 yielded positive cultures, revealing 61 bacterial species, predominantly Escherichia coli (22%), Staphylococcus aureus (16%), and Pseudomonas aeruginosa (9%). Gram-negative bacteria constituted 70.20% of the isolates. High antibiotic susceptibility rates were observed for Vancomycin (98%) and Linezolid (98%) in Gram-positive bacteria, while Amikacin (86%) and Meropenem (74.74%) were effective against Gram-negative bacteria. Notable resistance was found in Methicillin-resistant S. aureus (81%) and E. coli resistant to third-generation Cephalosporins (55%). These findings underscore the importance of monitoring bacterial patterns and antibiotic resistance for improved infection management at dr. Zainoel Abidin General Hospital, Banda Aceh.
Temperature Effects on Jembrana Superficial Unit (JSU) Recombinant Protein Expression in Escherichia coli BL21 Maelani, Imelda; Indriawati, Indriawati; Ismail, Yulia Sari; Mulyani, Riska; Syahfitri, Widya
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64590

Abstract

The efficiency of expression of recombinant proteins in host cells depends on achieving proper protein folding, solubility, and biological activity. Critical environmental factors, including temperature, pH, nutrient availability, and inducer concentration, significantly influence protein yield, stability, and functionality. Optimizing these conditions is essential to minimize protein misfolding and aggregation, which can adversely affect expression efficiency and reduce bioactivity. This study aims to measure the effect of temperature on the growth of Escherichia coli BL21 harboring the recombinant JSU protein, derived from the ENV-SU gene of the Jembrana virus. A controlled laboratory experiment was conducted in which bacterial cultures were incubated at 25°C and 37°C under identical conditions, except for temperature. The results indicate a statistically significant difference in bacterial growth between the two temperature conditions (p = 0.08), suggesting that E. coli BL21 exhibits enhanced growth at 37°C compared to 25°C. The mean bacterial growth rate at 37°C was 1.756, whereas at 25°C, the average growth was 1.425, demonstrating that higher temperatures facilitate bacterial proliferation. However, due to the relatively small sample size, further validation with a larger dataset is required to confirm these findings. These findings highlight the critical role of temperature optimization in enhancing bacterial growth and improving recombinant protein production efficiency, emphasizing the need for further investigation with a larger dataset to validate the results. Keywords: recombinant protein; temperature; IPTG; Escherichia coli BL21; JSU construct.
UJI EFEK NEFROPROTEKTIF EKSTRAK ETANOL DAUN BIWA (Eriobotrya japonica Lindl) TERHADAP KADAR KREATININ TIKUS MODEL KANKER PAYUDARA YANG DIINDUKSI Benzo(a)Pyrene Syahfitri, Widya; Elizabeth, Fitri; Putri, Syarifah Riska Mela; Maelani, Imelda; Hutahaean, Salomo; Ilyas, Syarifuddin
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.11120

Abstract

Benzo(a)pyrene (BaP) merupakan senyawa toksik dan karsinogenik yang dapat menyebabkan stres oksidatif, peradangan, dan gangguan fungsi organ, termasuk ginjal. Penelitian ini bertujuan untuk menguji efek nefroprotektif ekstrak metanol daun biwa (Eriobotrya japonica) terhadap kadar kreatinin tikus model kanker payudara yang diinduksi BaP. Penelitian ini menggunakan rancangan acak lengkap dengan lima kelompok perlakuan, yaitu kontrol negatif (tanpa perlakuan), kontrol positif (BaP 50 mg/kg BB), serta tiga kelompok perlakuan dengan pemberian ekstrak daun biwa dosis 200, 300, dan 400 mg/kg BB selama 30 hari. Kadar kreatinin diukur menggunakan metode modifikasi Jaffe. Data hasil penelitian dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa kadar kreatinin serum darah tikus pada semua kelompok masih berada dalam rentang normal, dengan nilai antara 0,66 hingga 0,73 mg/dl, dan tidak terdapat perbedaan yang signifikan (p0,05) antara kelompok perlakuan dan kontrol. Hal ini mengindikasikan bahwa paparan BaP tidak secara langsung mengganggu fungsi ginjal tikus model kanker payudara, serta pemberian ekstrak daun biwa tidak menunjukkan efek protektif yang signifikan terhadap kadar kreatinin. Namun, kandungan antioksidan dalam daun biwa, seperti flavonoid dan triterpenoid, tetap berpotensi dalam menjaga stabilitas fungsi ginjal melalui mekanisme antioksidatif dan antiinflamasi
Co-Authors Agung Pranata Aksa, Rahmad Ayulinda, Arianisah P. Elizabeth, Fitri Fathima, Raisha Firzan Nainu Harapan Harapan Ichsan Ichsan Ikram Ikram Ilyas, Syarifuddin Indriawati, Indriawati Jayawarsa, A.A. Ketut Kamil, Qatrunnada KEUMALAHAYATI, MEUTIA Latief, Kamaluddin Mahdani, Wilda Maimun Syukri, Maimun Mellinia, Sania A. Mudatsir Mudatsir MULIANA, DESI Mulyani, Riska Mutiara, Suci Nalapraya, Widhy Y. Nanda, Cut M. Nirwana, Aura PUTRI, FARAH DHITA ANISSA Putri, Syarifah Riska Mela Putri, Widia RAHMAWATI, CUT VIKA Ridwan, Azwar Rudi Kurniawan Salomo Hutahaean Samsul Anwar Sarifuddin Sarifuddin Sofyan, Sarwo E. Syahfitri, Widya Syahraini, Aigia Yesi Astri Yulia Sari Ismail Zinatul Hayati