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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar Biogenesis: Jurnal Ilmiah Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA)
Junairiah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

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Antioxidant potential of ethanol, ethyl acetate, n-hexane extracts from leaves, fruits, stems, and roots of red okra (Abelmoschus esculentus) Putri, Salsabilla Wiyana; Hapsari, Lukiteswari Dyah Tri; Junairiah; Wahyuningsih, Sri Puji Astuti
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.43666

Abstract

Abelmoschus esculentus L. Moench has been known for its abundant and affordable antioxidant potential. This plant has different antioxidant levels regarding the parts of the plant. Red okra is rich in flavonoids and polyphenols, including strong antioxidant as quercetin ad anthocyanin. This research is intended to determine the IC50 value and compare the total content of phenolic and flavonoid from ethanol, ethyl acetate, and n-hexane extract of red okra (A. esculentus) leaves, fruits, stems, and roots. Extraction process was done by gradual maceration method using ethanol, ethyl acetate, and n-hexane. IC50 value interprets the antioxidant activity obtained from DPPH test. The results showed a very strong antioxidant activity from red okra stem extract with IC50 of 25.22 ppm and the lowest from fruit n-hexane extract with 821.55 ppm. From TPC test, the highest content was obtained from stem ethanol extract with 156.01 mg GAE/g and the lowest from stem n-hexane extract with 25.99 mg GAE/. From TFC test, the highest content was obtained from fruits ethanol extract with 108.15 mg QE/g and the lowest from stem ethanol extract with 2.26 mg QE/g. In conclusion, this research showed that red okra (A. esculentus) contains a high level of phenol and flavonoid compound and the highest antioxidant content was shown in ethanol extract.
Utilization of Chitosan Elicitation on Biomass, Total Flavonoids, and Antioxidant Activity of Black Betel Callus (Piper betle L. var Nigra): Utilization of Chitosan Elicitation on Black Betel Callus (Piper betle L. var Nigra) Nurindah Wanti, Yulia Tri; Wida Utami, Edy Setiti; Junairiah
Journal of Tropical Life Science Vol. 15 No. 2 (2025): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/3d65dk31

Abstract

Callus culture is a suitable method for the production of secondary metabolites. Elicitors can increase the content of secondary metabolites through the elicitation process. This study aims to determine the effect of chitosan elicitation on biomass, total flavonoids, and antioxidant activity of black betel callus. The research method is callus production by growing leaf explants on Murashige and Skoog (MS) medium with the addition of growth regulators Benzyl Amino Purin (BAP) 0.5 mg/L and 2,4-Dichlorophenoxy Acetic Acid (2,4-D) 2.0 mg/L. Furthermore, the callus was sub-cultured on MS medium with chitosan elicitor for four days. The data obtained was callus biomass. Extraction of callus to measure flavonoid content and antioxidant activity. The results showed that chitosan elicitation at 1.5 mg/L showed the most optimal biomass results, 1.22  ± 0.39 g fresh weight and 0.81 ± 0.17 g dry weight. Chitosan elicitation with 1.5 mg/L treatment showed the highest total flavonoid content of 9.52 ± 0.37 µg QE/g. The methanol extract of black betel callus resulting from chitosan elicitation has antioxidant activity. Callus treated with 2 mg/L chitosan showed the most substantial IC50 value of 53.60 ± 14.45 mg/L. Chitosan elicitation can effectively increase biomass, total flavonoids, and antioxidant activity of black betel leaf callus. Chitosan 1.5 mg/L is the optimum concentration for increasing biomass and total flavonoids, and chitosan 2 mg/L is the concentration that has the most effect on the antioxidant activity of black betel leaf callus.
Evaluation of Biofertilizer on Growth and Productivity of Solanum lycopersicum L. Febrionny, Princessa Nandita; Azzahra, Annisa; Salsabila, Syarifah; Agustin, Niken; Tamalia, Aqilla Kalyca; Anjani, Ajeng Distya; Junairiah; Fatimah
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53696

Abstract

The increasing demand for tomatoes has prompted farmers to use chemical fertilizers, which can lead to soil degradation and reduced fertility. This study aims to determine the appropriate dose and frequency of biofertilizer application to enhance the growth and productivity of tomato plants (Solanum lycopersicum L.). The experiment was designed using a Completely Randomized Design (CRD) with 11 treatments, including both negative and positive controls. Three variations in biofertilizer doses were tested (5, 10, and 15 ml/plant), along with three different frequencies of application: once at one week after planting (1 WAP), twice (at 1 and 4 WAP), and three times (at 1, 4, and 8 WAP). Measured parameters included soil fertility (microbial population and functional microbial quality), plant growth (plant height, leaf count, stem diameter), and plant productivity (flower count). Quality of functional microbes were analyzed descriptively. Data of microbial population, plant growth and productivity were analyzed using Kruskal-Wallis and Mann-Whitney tests with a 5% significance level. The results showed significant differences in soil microbial populations and plant height between treatments, although no significant differences were observed in stem diameter, leaf number, or flower number. The treatment with 15 ml/plant applied three times (B15F3) exhibited the highest Relative Agronomic Efficiency (RAE) compared to other biofertilizer treatments (74.63%), but it was still lower than the control (chemical fertilizer). Therefore, higher doses and/or frequencies may be needed to further evaluate the effectiveness of the biofertilizer under test.
Biodiversity and Relationships in Species Annonaceae Using the Phenetic Method in the Purwodadi Botanical Garden Hamidah; Junairiah; Akustia, Putri
Jurnal Penelitian Pendidikan IPA Vol 11 No 4 (2025): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i4.10808

Abstract

This study aims to analyze the morphological diversity and phylogenetic relationships of six species from the Annonaceae family at the Purwodadi Botanical Garden using a phenetic approach. The background of this research is based on the importance of character-based identification to support taxonomy, conservation, and plant classification within the Annonaceae family, which is known for its high diversity. The research began with field exploration and specimen identification, followed by morphological characterization focusing on stem, leaf, petiole, and flower traits. Data acquisition was conducted by direct observation and measurement using tools such as calipers, rulers, and color tables. The morphological data were analyzed using Hierarchical Cluster Analysis and Principal Component Analysis (PCA) with SPSS 25.00 software to determine phylogenetic relationships among the species. The results revealed two major clusters: the first includes Mitrephora polyprena, Stelechocarpus burahol, Orophea enneandra, and Polyalthia bullata, with a similarity index of 73.2%, and the second consists of Annona muricata and Miliusa horsfieldii, with 59.3% similarity. PCA results indicated that leaf width, leaf length, petiole length, leaf color, and stem surface were the most influential morphological traits. These findings demonstrate that the phenetic approach is effective for analyzing phylogenetic relationships and can contribute to conservation strategies and biodiversity management.
Co-Authors Akustia, Putri Anjani, Ajeng Distya Annisa Azzahra, Annisa Fatimah Febrionny, Princessa Nandita HAMIDAH Hapsari, Lukiteswari Dyah Tri Niken Agustin Nurindah Wanti, Yulia Tri Putri, Salsabilla Wiyana Salsabila, Syarifah Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Tamalia, Aqilla Kalyca Wida Utami, Edy Setiti