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All Journal Jurnal Biologi Tropis Konservasi Hayati
Durotun Ainiyah
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Morphological and Physiological Adaptations of Venus Slipper (Paphiopedilum) Durotun Ainiyah; Nur Kholiq; Isfina Nurfaidatur Rohmah; Su’udi, Mukhamad
Konservasi Hayati Vol 20 No 1 (2024): APRIL
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v20i1.32147

Abstract

Paphiopedilum, commonly known as venus slipper or lady slipper, is a genus of orchids that boasts over 70 species, most of which are critically endangered to endangered. These orchids are characterized by the presence of a sac on the flower that serves a unique function closely related to pollination. The pollination of Paphiopedilum is facilitated by bees, which are lured to the flower by producing pheromones such as €-β-farnese and β-pinene. Pouched orchids also produce compounds from the terpenoid group to synthesize a scent that attracts pollinators. Paphiopedilum orchids have evolved a fascinating mechanism to ensure successful pollination. They trap pollinators by making the pollination area slippery so that when the pollinator enters the area, it is likely to slip and try to get out along with the attached pollen. This unique mechanism ensures that the pollen is transferred effectively, increasing the chances of successful fertilization.
DNA Barcode Analysis of Dendrobium sp. Based On rbcL Sequence Dwinianti, Edia Fitri; Durotun Ainiyah; Yusfi Afidah; Mukhamad Su'udi
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10177

Abstract

Morphological characterization of Dendrobium orchids needs to be supported by the utilization of DNA barcoding as a faster and more accurate approach. This study aims to identify the rbcL marker as a potential barcode for Dendrobium sp. collected from Jenggawah, Jember. The isolation of Dendrobium sp. genomic DNA was performed using the CTAB method, and the isolated DNA was amplified using PCR. The PCR products were used for sequencing analysis, and the sequence data were analyzed using several bioinformatics programs namely BLAST, ClustalX 2.1, and MEGA11. The results showed that the Dendrobium sp. sequence had a high level of homology with Calanthe alpina (MN192480.1), with a genetic similarity of 99.82%. The sequence analysis results of rbcL in Dendrobium sp. showed low genetic variation, which made rbcL less effective for use as a molecular marker at the species level. The rbcL barcode showed universal results for Dendrobium sp., so it needs to be supported by molecular identification using other markers such as matK and ITS for a more effective identification method in distinguishing between species.
Co-Authors Dwinianti, Edia Fitri Isfina Nurfaidatur Rohmah Mukhamad Su'udi Nur Kholiq Su’udi, Mukhamad Yusfi Afidah