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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Biologi Tropis
Mukhamad Su'udi
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Plant Breeding Techniques in Tissue Culture to Improve the Quality of Orchids Mukhamad Su'udi; Rosida, Waki’atil; Ravitamala, El Shania; Siddiq, Arif Mohammad; Setiawan, Rendy; Setyati, Dwi; Ningrum, Asyifa Yasmin; Roziqoh, Wasiatur
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 1 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.1.116

Abstract

Conventional orchid cultivation is thought to be ineffective and time-consuming. Simple tissue culture procedures are insufficient to increase orchid quality. The need for ongoing development can be met by integrating different plant breeding strategies in orchid tissue culture, such as induction mutation, elicitor addition, and genetic transformation. The purpose of this article was to report on current improvements in the use of plant breeding techniques to orchid tissue culture to increase orchid quality. This article referenced significant scientific publications. Orchid tissue culture using mutation induction is used to develop improved variations. Giving elicitors can cause orchid plants to conserve themselves and become more resistant to diseases. The required transgene can be introduced into the genome of cultivated orchids via Agrobacterium. These advancements have the potential to revolutionize orchid cultivation. Keywords: biotechnology, orchid, tissue culture
DNA Barcode Analysis of Dendrobium sp. Based On rbcL Sequence Dwinianti, Edia Fitri; Durotun Ainiyah; Yusfi Afidah; Mukhamad Su'udi
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10177

Abstract

Morphological characterization of Dendrobium orchids needs to be supported by the utilization of DNA barcoding as a faster and more accurate approach. This study aims to identify the rbcL marker as a potential barcode for Dendrobium sp. collected from Jenggawah, Jember. The isolation of Dendrobium sp. genomic DNA was performed using the CTAB method, and the isolated DNA was amplified using PCR. The PCR products were used for sequencing analysis, and the sequence data were analyzed using several bioinformatics programs namely BLAST, ClustalX 2.1, and MEGA11. The results showed that the Dendrobium sp. sequence had a high level of homology with Calanthe alpina (MN192480.1), with a genetic similarity of 99.82%. The sequence analysis results of rbcL in Dendrobium sp. showed low genetic variation, which made rbcL less effective for use as a molecular marker at the species level. The rbcL barcode showed universal results for Dendrobium sp., so it needs to be supported by molecular identification using other markers such as matK and ITS for a more effective identification method in distinguishing between species.
Co-Authors Afidah, Yusfi Arif Mohammad Siddiq Durotun Ainiyah Dwi Setyati Dwinianti, Edia Fitri Farrennina, Tasya Preira Mustafidah, Qori’atul Ningrum, Asyifa Yasmin Putri, Aura Balqis Nicheta Ravitamala, El Shania Rendy Setiawan Rosida, Waki’atil Roziqoh, Wasiatur Sani, Luthfiana Mutiara Yang, Haoling Yusfi Afidah