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All Journal PROSIDING SEMINAR NASIONAL Jurnal Biologi Tropis
Yusfi Afidah
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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PENGARUH WAKTU PEMBERIAN PUPUK KANDANG TERHADAP PERTUMBUHAN VEGETATIF TABULAMPOT JAMBU AIR MDH (Syzygium samarangense (Blume) Merr. & L.M. Perry) Yusfi Afidah; Fatimatuz Zuhro; Hasni Ummul Hasanah; Sugeng Winarso; Mohammad Hoesain
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.472 KB)

Abstract

MDH water apple is one type of fruit plant that can be cultivated intabulampot. Facts in the field indicate that planting it in tabulampotmakes the plant bear fruit faster. Another factor that determines theproduction of MDH guava is fertilization, including fertilizing withorganic matter. Besides giving organic fertilizer, the right time whenapplying fertilizer is also important in determining the growth of MDHguava. The purpose of this study was to determine the effect of the timeof manure on the vegetative growth of MDH guava. This research wasconducted in the green house in March to August 2018. This study useda randomized block design with 9 repetitions and 3 treatments, namely:(1) fertilization at the beginning of planting, (2) fertilization in the 1stand 3rd month after planting, and (3) fertilization in the 1st and 5thmonths after planting. Data were analyzed using ANOVA test. Theresults of this study indicate that manure has a significant effect on thenumber of leaf parameters, but does not significantly affect plantheight, number of primary branches, and number of secondarybranches. The best growth results were obtained at two time offertilizing in MDH water apple. Keyword : MDH water apple, manure, tabulampot, time of fertilizing.
DNA Barcode Analysis of Dendrobium sp. Based On rbcL Sequence Dwinianti, Edia Fitri; Durotun Ainiyah; Yusfi Afidah; Mukhamad Su'udi
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10177

Abstract

Morphological characterization of Dendrobium orchids needs to be supported by the utilization of DNA barcoding as a faster and more accurate approach. This study aims to identify the rbcL marker as a potential barcode for Dendrobium sp. collected from Jenggawah, Jember. The isolation of Dendrobium sp. genomic DNA was performed using the CTAB method, and the isolated DNA was amplified using PCR. The PCR products were used for sequencing analysis, and the sequence data were analyzed using several bioinformatics programs namely BLAST, ClustalX 2.1, and MEGA11. The results showed that the Dendrobium sp. sequence had a high level of homology with Calanthe alpina (MN192480.1), with a genetic similarity of 99.82%. The sequence analysis results of rbcL in Dendrobium sp. showed low genetic variation, which made rbcL less effective for use as a molecular marker at the species level. The rbcL barcode showed universal results for Dendrobium sp., so it needs to be supported by molecular identification using other markers such as matK and ITS for a more effective identification method in distinguishing between species.
Co-Authors Durotun Ainiyah Dwinianti, Edia Fitri Fatimatuz Zuhro Hasni Ummul Hasanah Hoesain, Mohammad Mukhamad Su'udi Sugeng Winarso