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All Journal Jurnal Biologi Tropis Jurnal Biosense
Ramadhan, Bintang Fadhil
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health

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Biblometric Analysis of the Effect of Type II Diabetes Mellitus on Immunity Husnia, Attahiyatul; Ramadhan, Bintang Fadhil; Yuniarti, Elsa
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8776

Abstract

Type 2 diabetes mellitus is the inability of the body's cells to respond to insulin or so-called insulin resistance which causes hyperglycemia. The body's immune system is a complex system that functions to protect the body from various diseases and infections. This research was conducted with the aim of applying bibliometric methods using quantitative analysis to trace the development of research related to type 2 Diabetes Mellitus. This study uses a biblometric analysis method that can help researchers in studying the content of bibliography, citation analysis of each article taken from the lens database. The results of data with the keywords DM type 2, immune, and complication are not too much, only about 475 data were obtained, and after being identified and cleaned using vosviewer, data was obtained in the form of several clusters such as images.
DESAIN PRIMER MMP-8 dan IL-6 UNTUK ANALISIS EKSPRESI GEN JARINGAN GUSI Rattus norvegicus DENGAN qRT-PCR Yasmine Dwinda; Farikh, Muhammad; Tiranissa, Vraya; Sari, Windi Yunita; Ramadhan, Bintang Fadhil; Farma, Siska Alicia
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.7222

Abstract

Matrix Metalloproteinase 8 (MMP-8) and Interleukin 6 (IL-6) are two important genes that mediate inflammation and play a role in inflammatory cases. This study aims to design two specific primer candidates for testing gene expression using the qRT-PCR method in Rattus norvegicus experiencing inflammation and gingivitis. Primer design was performed using Geneious Prime software and BLAST primers to determine primer quality through PCR product size, primer length, melting temperature (Tm), and %GC, as well as to ensure that the resulting primers only bind to the Rattus norvegicus genome target. The results showed that the size of the MMP-8 forward product CGGGGTATTGGAGGAGATGC; reverse CAGGGTTGTCTGAAGGTCCATA was 241 bp with a primer length of 20-22 nucleotides, %GC between 50-60%, and Tm not more than 60°C, and the size of the IL-6 product forward AGAGACTTCCAGCCAGTTGC; reverse TGCCATTGCACAACTCTTTTC is 199 bp with a primer length of 20-21 nucleotides, %GC between 42.86-55% and Tm not more than 60°C. BLAST primer results indicate that both primer pairs are specific and suitable as candidate primers for qRT-PCR testing in studies of inflammation-related gene expression and gingival inflammation.
Co-Authors Elsa Yuniarti Farikh, Muhammad Husnia, Attahiyatul Sari, Windi Yunita Tiranissa, Vraya Yasmine Dwinda