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All Journal Lantanida Journal Jurnal SOLMA Jurnal Riset Sains dan Kimia Terapan
Aurellia, Dema Griseldis
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Chemical Engineering, Chemistry & Bioengineering Chemistry Education Other

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In Vitro Evaluation of the Antibacterial Effect of Virgin Coconut Oil Against Pseudomonas aeruginosa Associated with Folliculitis Hati, Futi Kusuma; Rismaya, Rina; Aurellia, Dema Griseldis
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 1 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 1, Juni 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.111.05

Abstract

Virgin Coconut Oil (VCO) is a pure coconut oil rich in lauric acid, giving it potential antibacterial properties. Previous studies have successfully produced a VCO using a combined fermentation and enzymatic method with Neurospora sitophila, Lactobacillus plantarum, and papain. Nevertheless, further investigation is required to determine the antibacterial efficacy of the VCO against Pseudomonas aeruginosa, a bacterium responsible for folliculitis. This study analyzed of the antibacterial activity of VCO and Hydrolyzed Virgin Coconut Oil (HVCO) against P. aeruginosa in vitro. VCO is obtained by fermenting coconut milk using N. sitophila, L. plantarum and papain, then VCO undergoes hydrolysis with the aid of Lipozyme TL IM to yield HVCO. Inhibition zone assays were conducted to assess the antibacterial activity of 100% VCO and HVCO at varying concentrations of 25%, 50%, and 100%. The largest inhibition zone was shown by 100% HVCO (9.5 ± 0.3 mm in diameter), which was statistically significantly different from the control, ampicillin trihydrate. The data suggested that HVCO has enhanced antibacterial effectiveness against P. aeruginosa, compared to VCO. This increased activity is attributed to its higher free fatty acids and monolaurin content.
Pelatihan Pemanfaatan Limbah Kulit Buah Naga sebagai Antioksidan dalam Pembuatan Lip balm di Kabupaten Majalengka Hati, Futi Kusuma; Kartika, Irma Ratna; Saputra, Irwan; Listiara, Aulia; Aurellia, Dema Griseldis; Aisyafitri, Afifah; Azahra, Nur Fadillah Adelia; Albana, Fadhila Shofi
Jurnal SOLMA Vol. 14 No. 3 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v14i3.20994

Abstract

Background: Kulit buah naga merupakan limbah organik yang kaya akan antosianin dan berpotensi sebagai antioksidan alami untuk perawatan kulit, namun pemanfaatannya dalam produk perawatan kulit, seperti lip balm masih terbatas. Studi ini bertujuan memberikan sosialisasi dan pelatihan kepada siswa SMP di Kabupaten Majalengka mengenai inovasi pemanfaatan limbah kulit buah naga dalam pembuatan lip balm. Metode: Kegiatan mencakup sosialisasi manfaat buah naga dan lipbalm, demonstrasi, praktik, diskusi tanya jawab dan evaluasi. Sebanyak 43 siswa SMPIT Tazkia Insani Majalengka mengikuti kegiatan ini. Para peserta dilatih mengelola limbah kulit buah naga menjadi lip balm dan mempraktikannya langsung sehingga turut mengurangi sampah di lingkungan sekitar dan menumbuhkan kreativitas serta keterampilan dalam menciptakan produk ramah lingkungan. Hasil: Peserta berhasil membuat lipbalm dari limbah kulit buah naga serta memahami proses dan bahan yang digunakan. Kesimpulan: Program penguatan sistem informasi website mampu meningkatkan aksesibilitas informasi dan partisipasi warga RT 39. Keberhasilan ini menunjukkan pentingnya kolaborasi antara pengurus RT dan kelompok ibu rumah tangga dalam mewujudkan masyarakat yang adaptif terhadap teknologi.
CHEMICAL PROFILING AND ANTIBACTERIAL ACTIVITY OF Nannochloropsis oculata EXTRACTS AGAINST FOODBORNE PATHOGENS Hati, Futi Kusuma; Nurjayadi, Muktiningsih; Kartika, Irma Ratna; Azahra, Nur Fadillah Adelia; Albana, Fadhila Shofi; Aisyafitri, Afifah; Aurellia, Dema Griseldis
Lantanida Journal Vol 14 No 1 (2026): January-June 2026
Publisher : UIN AR-RANIRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/f2h2ht06

Abstract

Foodborne illnesses remain a public health concern in Indonesia, prompting the search for natural antibacterial agents. This study evaluated the antibacterial activity and chemical profile of Nannochloropsis oculata extracts against Listeria monocytogenes and Shigella flexneri. Microalgae were cultivated, and cell density was monitored using an Ultraviolet–Visible (UV–Vis) spectrophotometer. Biomass was harvested and extracted using chloroform–methanol, followed by compound identification through Gas Chromatography–Mass Spectrometry (GC–MS). Antibacterial activity was assessed using the disc diffusion method. A total of 1.3196 g of dry biomass was obtained. At 200 mg/mL, the extract produced inhibition zones of 8.40 ± 0.17 mm against L. monocytogenes and 8.83 ± 0.29 mm against S. flexneri. GC–MS analysis revealed fatty acid derivatives, predominantly methyl palmitoleate (26.47%). These findings provide preliminary insight into the antibacterial potential of N. oculata extracts; however, further quantitative and application-based studies are required to determine their practical relevance.
Co-Authors Aisyafitri, Afifah Albana, Fadhila Shofi Azahra, Nur Fadillah Adelia Hati, Futi Kusuma Kartika, Irma Ratna Listiara, Aulia Muktiningsih Nurjayadi Rina Rismaya Saputra, Irwan