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All Journal Biology, Medicine, & Natural Product Chemistry Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian
Napitupulu, Beatrix Sofranes
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

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Potensi Limbah Gulma Alang-alang dan Saliara sebagai Bioherbisida untuk Pengendalian Gulma secara Pra Tumbuh Sari, Vira Irma; Napitupulu, Beatrix Sofranes; Christy, Julieta; Nafisah, Rouzatul
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 9 No. 2 (2025): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, 9 (2) Juni 2025
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v9i2.15165

Abstract

Alang-alang (Imperata cylindrica) and Saliara (Lantana camara) are dominant weeds in several crop cultivation areas, so that when control is carried out, waste from both weeds will be produced in large quantities. Management of waste from both weeds can be done by utilizing it as a more environmentally friendly bioherbicide. Bioherbicide application is carried out pre-growth so that control is carried out faster and attacks newly germinated weed seeds, so that weeds do not appear on the surface and weed control activities will be more efficient. Alang-alang is the most dangerous weed and is highly avoided because it contains allelochemical compounds that can inhibit the growth of the main plant, this compound is found in all parts of the weed but is found most in the roots. Saliara also has a high content of allelochemical compounds which causes a distinctive aroma from its leaves. The allelochemical content of both weeds can be used as active ingredients in bioherbicides and is effective in controlling other weed species. Several studies have reported that bioherbicide extracts from both weeds are effective in inhibiting weed growth so that they can be used as an alternative to herbicides. However, bioherbicide application must be carried out with the right technique and concentration so as not to interfere with and inhibit the growth of the main plant.
Isolation, Morphological Characterization, Biochemistry, and Antagonistic Activity of Endophytic Bacteria from Rice Against Xanthomonas oryzae pv. Oryzae Sihotang, Saipul; Butar-Butar, Alfons Dwi Putra; Nasution, Lita; Rahma, Haliatur; Trizelia, Trizelia; Saragih, Magdalena; Kuswardani, Retna Astuti; Listiana, Ika; Napitupulu, Beatrix Sofranes
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.825-829

Abstract

Endophytic bacteria have the potential as environmentally friendly biocontrol agents in controlling bacterial leaf blight (BLB) in rice caused by Xanthomonas oryzae pv. oryzae (Xoo). This study aims to isolate, characterize morphology and biochemical tests, and test the antagonistic activity of endophytic bacteria from rice against Xoo. Isolation was carried out from healthy rice root tissue using a surface sterilization method. Morphological characterization included colony shape, color, edge, elevation, and Gram properties, while biochemical characterization included catalase, oxidase, motility, starch hydrolysis, and sugar fermentation tests. The antagonistic test used a dual culture assay method on NA (nutrient agar) media. The isolation results obtained six endophytic bacterial isolates (BETP 01–06) identified as the genus Pseudomonas and Bacillus with varying colony morphological characters. Antimicrobial activity test showed two potential isolates, BETP 03 (Pseudomonas) and BETP 05 (Bacillus), with an average inhibitory diameter of 16.3 mm and 16.5 mm against Xoo, respectively. Biochemical characterization showed that BETP 03 was bacillus, Gram-negative, citrate-negative, motile, gelatin hydrolysis positive, starch hydrolysis positive, and catalase positive; while BETP 05 was bacillus, Gram-positive, citrate-positive, motile, gelatin hydrolysis negative, starch hydrolysis positive, and catalase positive. These results indicate that both isolates have high potential as biological biocontrol agents in controlling bacterial leaf blight disease in rice.
Co-Authors Butar-Butar, Alfons Dwi Putra Christy, Julieta Haliatur Rahma, Haliatur Ika Listiana Nafisah, Rouzatul Nasution, Lita Retna Astuti Kuswardani Saipul Sihotang Saragih, Magdalena Trizelia . Vira Irma Sari, Vira Irma