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Profil GCMS Senyawa Kimia Ekstrak Metanol Isolat Cendawan Entomopatogen Beauveria Bassiana Dan Akar Cabai Sebagai Pemacu Pertumbuhan Cabai Saragih, Magdalena; Trizelia, Trizelia; Nurbailis, Nurbailis; Yusniwati, Yusniwati
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol 4, No 2 (2020): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

The aim of this study was to identify the chemical compound methanol extract of entomopathogenic fungus Beauveria bassiana from insect walang sangit and the chemical compound roots of red chili plants that were able to stimulate the growth of chilli plants after being applied with entomopathogenic fungus Â Â B. bassiana through seed immersion inoculation using GCMS method. The chemical compound identified as a growth booster in Â Â B. bassiana fungus isolates from the insect walang sangit is an Acetic acid Ethanoic acid Ethylic acid Glacial acetic acid CH3COOH, Hexadecanoic acid, methyl ester (CAS) Methylpalmitate Uniphat A60, n-Hexadecanoic acid Hexadecanoic acid n-Hexadecoic acid Palmitic acid, Dianhydromannitol and Ergosta-5,7,22-trien-3-ol, (3.beta., 22E) - (CAS) Ergosterol (CAS), while the chili root contains Acetic acid Ethanoic acid Ethylic acid Glacials acetic acid CH3COOH, Hexadecanoic acid, methyl ester (CAS) Methyl palmitate Uniphat A60, n -Hexadecanoic acid Hexadecanoic acid Palmitic acid, 8, 11- octadecadienoic acid, methyl ester (CAS) METHYL 8, 11-OCTADECADIENOATE, (23S)-ethylcholest-5-en-3.beta.-ol.s It can be concluded that some of the chemical compounds in methanol extracts identified in chili roots have similarities with chemical compounds that exist in Â Â B. bassiana entomopathogenic fungus which are potential as stimulers of chili plant growth

Cendawan entomopatogen sebagai penginduksi ketahanan tanaman: Sebuah tinjauan sistematis: Entomopathogenic fungi as plant resistance inducer: A systematic review Vajri, Indri Yanil; Trizelia, Trizelia; Kuswardani, Retna Astuti; Saragih, Magdalena
Jurnal Entomologi Indonesia Vol 21 No 1 (2024): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.21.1.75

IInsect pathologists' attention to the potential of entomopathogenic fungi (EPF) as inducers of plant resistance has increased in recent years. Several types of EPF that have received attention as biocontrol agents for insect pest populations include Beauveria bassiana, Metarhizium anisopliae and Trichoderma sp. because they are able to infect and kill insects directly, has a wide host range, and has been used to control various types of insect pests. Apart from being able to infect and kill insect pests, EPF can also live endophytically in plant tissue and increase plant resistance to pest attacks. The presence of EPF as endophytes is able to induce plant resistance by stimulating an increase phytohormones production. In this article, we reviewed the role of fungi as entomopathogens, the characteristics of entomopathogenic fungi, and their role as inducers of plant resistance. The review was carried out by collecting information from original articles and related reports which reviewed the results of research on the role of entomopathogenic fungi as plant resistance inducers by including the keywords entomopathogenic endophytic fungi, resistance induction, characterization, systemic resistance and herbivorous insects. This systematic review concludes that EPF as an inducer of plant resistance is a very promising control alternative in the development of plant protection management against insect pests.

Aplikasi Insektisida Sintetik dan Pestisida Nabati untuk Pengendalian Plutella Xylostella pada Tanaman Kubis (Brassica Oleraceae L) Yanty, Dini Puspita; Sembiring, Riduan; Sembiring, Swati; Saragih, Magdalena; Sinulingga, Riski Adi Vindo
All Fields of Science Journal Liaison Academia and Sosiety Vol 5, No 2: Juni 2025
Publisher : Lembaga Komunikasi dan Informasi Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58939/afosj-las.v5i2.1144

Budidaya kubis seringkali menghadapi banyak kendala dalam meningkatkan produktivitas baik dari segi kuantitas maupun kualitas. Salah satu masalah utamanya yaitu adanya serangan hama pengganggu tanaman kubis yaitu Plutella xylostella. Serangga ini merupakan jenis hama yang dapat merugikan cukup besar dan dapat menyebabkan gagal panen apabila tidak dilakukan pengendalian. Penggunaan pestisida insektisida sintetik dan pestisida nabati merupakan salah satu cara untuk mengendalikan serangan P. xylostella. Tujuan Penelitian adalah untuk mengetahui pengaruh aplikasi insektisida sintetik, pestisida nabati dan kombinasi perlakuan dalam pengendalian P. xylostella pada tanaman kubis (Brassica oleraceae, L). Penelitian ini dilaksanakan di lahan petani JI. Djamin Ginting. Perumahan Teropis Kecamatan Kabanjahe Kabupaten Karo dari Desember 2022 sampai dengan April 2023, menggunakan Rancangan Acak Kelompok (RAK) Faktorial dengan dua faktor perlakuan. Faktor pertama adalah Jenis insektisida (I) terdiri dari 2 taraf perlakuan yaitu: Insektisida Prevathon 50 SC (IP) dan insektisida Rotenon (IT). Faktor yang kedua adalah konsentrasi yang digunakan (K) yang terdiri dari 4 taraf perlakuan yaitu: tanpa insektisida (Ko), 1 ml/liter air (K1), 1.5 ml/liter air (K,) dan 2.0 ml/liter air (K3). Hasil penelitian menunjukkan bahwa jenis insektisida dan konsentrasi memberi pengaruh yang nyata terhadap penurunan populasi P. xylostella, intensitas serangan serta dapat menyelamatkan produksi dari serangan P. xylostella. Penggunaan insektisida Prevathon 50 SC (IP) dengan konsentrasi 1.5 ml/liter air (K2) dapat menekan serangan P. xylostella dan menyelamatkan produksi. Perlakuan kombinasi yang diujikan tidak menunjukkan pengaruh perbedaan yang nyata pada setiap parameter yang diamati.

Sosialisasi Pemeliharaan Tanaman Bunga Hias kepada Warga Desa Perumnas Simalingkar Kecamatan Pancur Batu Kabupaten Deli Serdang Sembiring, Swati; Saragih, Magdalena; Sembiring, Riduan; Ginting, Rajiv; Sihotang, Saipul; Silaban, Sri Rejeki; Pandiangan, Indera Prabowo
Journal Liaison Academia and Society Vol 5, No 2: Juni 2025
Publisher : Lembaga Komunikasi dan Informasi Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58939/j-las.v5i2.1153

Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan dan keterampilan warga Desa Perumnas Simalingkar, Kecamatan Pancur Batu, Kabupaten Deli Serdang, dalam hal pemeliharaan tanaman bunga hias sebagai upaya pemberdayaan ekonomi berbasis rumah tangga sekaligus pelestarian lingkungan. Latar belakang kegiatan ini berangkat dari potensi besar yang dimiliki oleh desa tersebut, yaitu kondisi iklim dan geografis yang mendukung pertumbuhan tanaman hias, namun belum dimanfaatkan secara optimal oleh masyarakat karena keterbatasan informasi dan keterampilan teknis. Kegiatan ini diikuti oleh Ibu PKK yang merupakan warga desa perumnas simalingkar kecamatan pancur batu kabupaten deli serdang. Hasil dari kegiatan ini menunjukkan bahwa peserta mengalami peningkatan pemahaman yang signifikan terhadap aspek teknis pemeliharaan bunga hias, yang diukur melalui pre-test dan post-test sederhana. Selain itu, peserta menunjukkan antusiasme untuk mulai menanam dan merawat bunga hias di pekarangan rumah masing-masing sebagai langkah awal menuju kegiatan usaha mikro berbasis tanaman hias. Kegiatan ini memberikan kontribusi nyata dalam membangun kesadaran masyarakat terhadap nilai estetika dan ekonomi dari tanaman hias serta mendorong inovasi pemanfaatan lahan pekarangan secara produktif. Dengan memanfaatkan teknologi digital dalam pelaksanaan sosialisasi, kegiatan ini membuktikan bahwa pengabdian kepada masyarakat tetap dapat dilakukan secara efektif dan efisien, meskipun dalam keterbatasan mobilitas. Keberlanjutan program ini diharapkan terjaga melalui pendampingan lanjutan dan pengembangan kelompok tani bunga hias secara swadaya masyarakat.

Isolation, Morphological Characterization, Biochemistry, and Antagonistic Activity of Endophytic Bacteria from Rice Against Xanthomonas oryzae pv. Oryzae Sihotang, Saipul; Butar-Butar, Alfons Dwi Putra; Nasution, Lita; Rahma, Haliatur; Trizelia, Trizelia; Saragih, Magdalena; Kuswardani, Retna Astuti; Listiana, Ika; Napitupulu, Beatrix Sofranes
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.825-829

Endophytic bacteria have the potential as environmentally friendly biocontrol agents in controlling bacterial leaf blight (BLB) in rice caused by Xanthomonas oryzae pv. oryzae (Xoo). This study aims to isolate, characterize morphology and biochemical tests, and test the antagonistic activity of endophytic bacteria from rice against Xoo. Isolation was carried out from healthy rice root tissue using a surface sterilization method. Morphological characterization included colony shape, color, edge, elevation, and Gram properties, while biochemical characterization included catalase, oxidase, motility, starch hydrolysis, and sugar fermentation tests. The antagonistic test used a dual culture assay method on NA (nutrient agar) media. The isolation results obtained six endophytic bacterial isolates (BETP 01–06) identified as the genus Pseudomonas and Bacillus with varying colony morphological characters. Antimicrobial activity test showed two potential isolates, BETP 03 (Pseudomonas) and BETP 05 (Bacillus), with an average inhibitory diameter of 16.3 mm and 16.5 mm against Xoo, respectively. Biochemical characterization showed that BETP 03 was bacillus, Gram-negative, citrate-negative, motile, gelatin hydrolysis positive, starch hydrolysis positive, and catalase positive; while BETP 05 was bacillus, Gram-positive, citrate-positive, motile, gelatin hydrolysis negative, starch hydrolysis positive, and catalase positive. These results indicate that both isolates have high potential as biological biocontrol agents in controlling bacterial leaf blight disease in rice.

Cendawan entomopatogen sebagai penginduksi ketahanan tanaman: Sebuah tinjauan sistematis: Entomopathogenic fungi as plant resistance inducer: A systematic review Vajri, Indri Yanil; Trizelia, Trizelia; Kuswardani, Retna Astuti; Saragih, Magdalena
Jurnal Entomologi Indonesia Vol 21 No 1 (2024): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.21.1.75

IInsect pathologists' attention to the potential of entomopathogenic fungi (EPF) as inducers of plant resistance has increased in recent years. Several types of EPF that have received attention as biocontrol agents for insect pest populations include Beauveria bassiana, Metarhizium anisopliae and Trichoderma sp. because they are able to infect and kill insects directly, has a wide host range, and has been used to control various types of insect pests. Apart from being able to infect and kill insect pests, EPF can also live endophytically in plant tissue and increase plant resistance to pest attacks. The presence of EPF as endophytes is able to induce plant resistance by stimulating an increase phytohormones production. In this article, we reviewed the role of fungi as entomopathogens, the characteristics of entomopathogenic fungi, and their role as inducers of plant resistance. The review was carried out by collecting information from original articles and related reports which reviewed the results of research on the role of entomopathogenic fungi as plant resistance inducers by including the keywords entomopathogenic endophytic fungi, resistance induction, characterization, systemic resistance and herbivorous insects. This systematic review concludes that EPF as an inducer of plant resistance is a very promising control alternative in the development of plant protection management against insect pests.

Effects of Naphthaleneacetic Acid and Kinetin on Chrysanthemum Plantlets in Vitro Sihotang, Saipul; Saragih, Magdalena; Sembiring, Swati; Sembiring, Riduan; Yanty, Dini Puspita; Tanjung, Denny Akbar; Afrida, Elli
Journal of Applied Agricultural Science and Technology Vol. 9 No. 4 (2025): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v9i4.315

Naphthaleneacetic acid (NAA) and kinetin play a crucial role in plant growth but must be used in correct proportions in order to produce the best outcomes. An optimal combination of both can enhance shoot, leaf, and root growth, whereas inappropriate doses may otherwise inhibit growth due to hormonal imbalance. This study examined the effects of the interaction between NAA and kinetin on the growth of chrysanthemum plants at eight weeks of age. The research was carried out using the Completely Randomized Design (CRD) in triplicate. The medium used was MS medium, supplemented with NAA at concentrations of 0–1.5 mg/L and kinetin at concentrations of 0–3 mg/L. The results indicate that NAA and kinetin positively influenced plant growth, but only when the optimal concentrations were applied. The best combination of NAA at 1.0 mg/L (N2) and kinetin at 3.0 mg/L (K3) produced the best results in terms of height (153 mm), number of leaves (31 leaves), number of roots (26 roots), and fresh weight (5.85 g). Increasing kinetin concentrations (K0-3) generally promoted plant growth, whereas excessive NAA concentrations (> 1.0 mg/L, N3) produced the opposite effect, likely due to toxicity or hormonal imbalance. The interaction between kinetin and NAA exhibited a synergistic effect up to a certain threshold, while excessive NAA application negatively impacted on plant development.

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