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All Journal Jurnal Analis Medika Biosains (JAMBS) Jurnal Sains Natural Indonesian Journal of Microbiology
Nisa, Shalehatun
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Health Professions Immunology & microbiology Mathematics Nursing Physics Public Health

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Optimasi Metode Baermann untuk deteksi Larva Soil Transmitted Helmints pada tanah di Tanah Bumbu Nurmansyah, Dian; Julpadli, Muhammad; Sasmitha, Maya Herliana; Humairah, Salsabila; Ansari, Muhammad Hafiz; Nisa, Shalehatun; Mudzakkir, Musyirah; Zalianty, Firda
Indonesian Journal of Microbiology Vol. 2 No. 2 (2025): July
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/ijm.v2i2.4618

Abstract

Penelitian ini bertujuan untuk mendeteksi keberadaan larva Soil Transmitted Helminths (STHs), khususnya hookworm, pada sampel tanah di Desa Manuntung, Kecamatan Kusan Hulu, Kabupaten Tanah Bumbu, Kalimantan Selatan. Wilayah ini dipilih berdasarkan kesamaan karakteristik geografis dan laporan prevalensi tinggi infeksi cacing di daerah sekitarnya. Desain penelitian ini adalah deskriptif dengan pendekatan observasional. Sebanyak 25 titik sampel tanah dikumpulkan dari dua lokasi berbeda, yaitu area persawahan (10 titik) dan area perkebunan kelapa sawit (15 titik). Sampel diambil pada kedalaman 3–5 cm dan diperiksa menggunakan metode Baermann di Laboratorium Mikrobiologi dan Parasitologi Universitas Borneo Lestari. Pemeriksaan difokuskan pada identifikasi larva hookworm berdasarkan morfologi mikroskopis, dengan analisis data disajikan secara deskriptif. Hasil penelitian menunjukkan bahwa seluruh sampel tanah dari persawahan (100%) dan sebagian besar dari perkebunan sawit (93,3%) positif mengandung larva hookworm. Karakteristik tanah yang gembur, berpasir, dan lembab di kedua lokasi tersebut diduga kuat mendukung pertumbuhan dan kelangsungan hidup larva. Metode Baermann terbukti efektif dalam mendeteksi larva STHs karena sensitivitas tinggi dan kemampuannya menangkap larva yang bergerak aktif di media cair. Kesimpulan dari penelitian ini menunjukkan adanya potensi tinggi penularan STHs kepada masyarakat sekitar, terutama kelompok yang bekerja di area pertanian dan perkebunan tanpa pelindung kaki. Hasil ini menjadi data awal penting untuk penelitian lanjutan pada manusia, serta mendukung perlunya intervensi berbasis perilaku dan sanitasi untuk menekan risiko infeksi kecacingan.
Lymphocyte Cell Count Profile based on Widal Titer On Pediatric Typhoid Fever Nurmansyah, Dian; Fayumi, Syihab; Nisa, Shalehatun; Puspawati, Puspawati; Sasmitha, Maya; Mudzakkir, Musyirrah; Arsyad, Muhammad
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.480

Abstract

Typhoid fever remains a significant public health concern in Indonesia, which has the third-highest global case rate, with 350–810 cases per 100,000 people and a fatality rate of 3.1–10.4%, primarily due to delayed diagnosis and treatment. Children aged 5–14 years are most affected, with transmission occurring via contaminated food, water, or poor hygiene. Salmonella typhi invades intestinal immune tissues (Peyer’s patches), triggering responses from T and B lymphocytes, though bacterial endotoxins can suppress bone marrow function, altering leukocyte levels. This study analyzed the relationship between Widal titer and lymphocyte counts in pediatric typhoid patients using a cross-sectional design. Data from 526 patients at Graha Medika Martapura Clinic and Ratu Zalecha Hospital (January 2023–February 2025) were analyzed statistically, with inclusion criteria of Widal titer ≥1/320 and complete blood count results. Results showed 18.8% of patients had elevated lymphocytes, 79.3% had normal levels, and 1.9% exhibited lymphocytopenia. Higher Widal titers (1/640) correlated with lower lymphocyte counts (15.8% elevated vs. 23.0% at 1/320), suggesting immune suppression mediated by IL-6 through specific signaling pathways. Paradoxically, some cases showed lymphocytosis (18.8%), likely due to cytokine-induced immune modulation. Statistical analysis revealed a minimal correlation (R²=0.006), indicating Widal titer explains only 0.6% of lymphocyte variation. These findings demonstrate that while Widal titer has limited predictive value for lymphocyte changes, immune response mechanisms, particularly involving IL-6, play a critical role in typhoid fever's hematological manifestations.
Profil Jumlah Leukosit Berdasarkan Nilai Titer Widal pada Kasus Demam Tifoid Anak Nurmansyah, Dian; Nisa, Shalehatun; Puspawati, Puspawati; Fayumi, Syihab; Mudzakkir, Musyirah; Sasmitha, Maya Herliana
JSN : Jurnal Sains Natural Vol. 3 No. 2 (2025): Agustus
Publisher : Puslitbang Sekawan Institute Nusa Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35746/jsn.v3i2.845

Abstract

Typhoid fever, caused by Salmonella typhi infection, remains a public health issue in developing countries, including Indonesia, with the highest prevalence among children aged 5–14 years. This study aimed to analyze the leukocyte profile of pediatric typhoid fever patients at Graha Medika Clinic, Banjar Regency. An observational analytic design with a cross-sectional approach was used. The sample included 526 pediatric patients with Widal titers ?1/320 who underwent complete blood tests. Results revealed that 71.3% of patients had normal leukocyte counts, while 22.4% showed leukocytosis and 6.3% had leukopenia. Simple linear regression analysis indicated no significant relationship between Widal titers and leukocyte levels (R² = 0.002). These findings suggest that most pediatric typhoid fever cases present with normal leukocyte counts. Among abnormal cases, leukocytosis was more common, though leukopenia was also observed.
Co-Authors Ansari, Muhammad Hafiz Fayumi, Syihab Humairah, Salsabila Julpadli, Muhammad Mudzakkir, Musyirah Mudzakkir, Musyirrah Muhammad Arsyad Puspawati, Puspawati Sasmitha, Maya Sasmitha, Maya Herliana Zalianty, Firda