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All Journal The Indonesian Biomedical Journal MAGNA MEDICA: Berkala Ilmiah Kedokteran dan Kesehatan Jurnal Ilmu Kedokteran dan Kesehatan
Indrasari, Setyarina
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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High-yield and Functional IgY Anti-S1 SARS-CoV-2 Delta Variant from Chicken Immunized with In-house Recombinant Protein Zarkasie, Kamaluddin; Ginting, Teridah Ernala; Poeloengan, Andrea Hynan; Andriani, Febi; Lages, Aksar Chair; Cornelia, Vina; Nugraha, Anggah; Rosidi, Bustanur; Indrasari, Setyarina; Nidom, Chairul Anwar; Yusuf, Irawan
The Indonesian Biomedical Journal Vol 17, No 4 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i4.3693

Abstract

BACKGROUND: During COVID-19 pandemic, effective preventive and therapeutic strategies was urgently necessary, especially with the emerging Delta variants. Previous existing interventions have some limitations, and passive immunization using immunoglobulin Y (IgY) is considered viable. However, the potential of IgY antibodies remains underexplored locally. Therefore, a feasible study was conducted to produce IgY against the SARS-CoV-2 Delta variant from chickens raised on a farm, instead of a laboratory environment, by using in-house recombinant protein, to offer a promising low-cost alternative for passive immunization.METHODS: In-house HEK293 cell line was produced by secreting recombinant S1 subunit protein of SARS-CoV-2 Delta variant. The protein was confirmed by western blot and used as an immunogen in two chickens. IgY was extracted from egg yolks. Titers and neutralization activity of IgY against SARS-CoV-2 Delta variant were confirmed by enzyme-linked immunosorbent assay (ELISA) and surrogate virus neutralization test (sVNT). The functionality of IgY was then tested on lysates from COVID-19 patients' nasopharyngeal swabs with dot blot method.RESULTS: The high anti-S1 IgY titers and neutralization activity was confirmed following immunization with 1 mg immunogen at 2–3-week intervals. IgY titers varied between chickens and time points. The mean titers showed significant increase after the fifth immunization (1.21, 1.72 and 1.48; with p<0.05 for all). Neutralization activity appeared after the second immunization and was significant after the third immunization (31.9%, 34.95%, 26.9%, 47.6%, 54.95% and 57.3%; with p<0.05 for all). The results of this study showed that the extracted IgY reacted to COVID-19 patients' nasopharyngeal swabs lysates.CONCLUSION: A high-yield and functional IgY anti-S1 SARS-CoV-2 Delta variant can be produced from chicken immunized with an in-house recombinant protein. This can be an alternative for affordable and effective IgY production during public health emergencies.KEYWORDS: IgY, SARS-CoV-2, S1 subunit protein, egg yolk, chicken
Acethylcholinesterase Levels of Aedes aegypti Larvae after Exposure to The Pandanus amaryllifolius Leaf Extracts Anin, Lidya; Adrianto, Hebert; Silitonga, Hanna Tabita Hasianna; Indrasari, Setyarina; Sari, Kartika Buana
MAGNA MEDIKA Berkala Ilmiah Kedokteran dan Kesehatan Vol 12, No 1 (2025): FEBRUARY
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/magnamed.12.1.2025.31-39

Abstract

Background:  Dengue fever is a disease transmitted by Ae. aegypti. Controlling mosquitoes can use temephos. Temephos larvicides act primarily on the enzyme acetylcholinesterase (AChE) by inhibiting this enzyme. Temephos has drawbacks due to its high risk of resistance, so larvicides from natural ingredients such as pandan leaf extract (P. amaryllifolius) can be an alternative. The mechanism of action of P. amaryllifolius larvicide is still unclear.Objective: This study aims to ascertain how LC85 pandan leaf methanol extract affects AChE levels in Ae. aegypti.Methods: This is an actual experiment design with only a post-test control group research design. Tests were conducted by treating mosquito larvae with P. amaryllifolius LC85 extract, aquades, and temephos for 24 hours and measuring AChE levels with an ELISA Reader.Results: The results showed that the AChE enzyme levels of P. amaryllifolius LC85 extract had an average AChE enzyme level of 147.19 + 70.87 units/l. The AChE enzyme levels of larvae exposed to P. amaryllifolius LC85, aquades, and temephos significantly differed (p <0.05).Conclusion: P. amaryllifolius has potential as a larvicidal, with a mechanism of action as a neurotoxin.
Keberadaan Toxoplasma gondii Dalam Daging Kambing Yang Dijual Di Pasar Surabaya Barat Berdasarkan Gen Bag-1 Tandean, Victor Setiawan; Adrianto, Hebert; Messakh, Billy Daniel; Yuwono, Victor Kurniawan; Juliasih, Ni Njoman; Santoso, Jemima Lewi; Putri, Firda Aulia; Indrasari, Setyarina
Jurnal Ilmu Kedokteran dan Kesehatan Vol 12, No 10 (2025): Volume 12 Nomor 10
Publisher : Prodi Kedokteran Fakultas Kedokteran Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jikk.v12i10.20687

Abstract

Toksoplasmosis adalah penyakit zoonosis yang disebabkan oleh parasit protozoa Toxoplasma gondii. Parasit dapat menginfeksi manusia dan semua hewan berdarah panas. Manifestasi parah berupa komplikasi yang berpotensi mengakibatkan kebutaan, yaitu Ocular toxoplasmosis (OT). T. gondii dapat ditularkan melalui konsumsi daging yang dimasak kurang matang. Tujuan penelitian adalah mendeteksi T. gondii di dalam daging kambing yang dijual di Surabaya Barat berdasarkan PCR gen BAG1. Penelitian ini adalah penelitian observasional deskriptif. 20 sampel daging kambing diambil dari beberapa pasar di Surabaya Barat dengan kuota sampling. Daging kambing diekstraksi, diamplifikasi PCR dengan gen BAG-1, dan dielektroforesis. Data hasil positif dan negatif dikelompokkan dan dianalisis secara deskriptif. Hasil pemeriksaan didapatkan 10% positif T. gondii dalam daging kambing berukuran 470 bp. Tidak mengkonsumsi daging kambing setengah matang dan memasak daging kambing dengan suhu tinggi dapat mencegah penularan T. gondii.
Co-Authors Aksar Chair Lages, Aksar Chair Andriani, Febi Anin, Lidya Billy Daniel Messakh Cornelia, Vina Ginting, Teridah Ernala Hanna Tabita Hasianna Silitonga Hebert Adrianto Irawan Yusuf Jemima Lewi Santoso Kamaluddin Zarkasie Ni Njoman Juliasih Nidom, Chairul Anwar Nugraha, Anggah Poeloengan, Andrea Hynan Putri, Firda Aulia Rosidi, Bustanur Sari, Kartika Buana Victor Kurniawan Yuwono Victor Setiawan Tandean