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All Journal Biotropika Jurnal Riset Biologi dan Aplikasinya
Damayanti, Ayu Elysia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

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Molecular Analysis and Morphological Characterization of Tor sp. using DNA Barcode COI from Telaga Rambut Monte, Blitar, East Java, Indonesia Damayanti, Ayu Elysia; Rabbani, Calista Naya; Sulung, Cahya Ajeng Valenta Tresna; Rusdianto, Rusdianto; Nugroho, Endik Deni; Mamat, Noorhidayah Binti; Rahayu, Dwi Anggorowati
Biotropika: Journal of Tropical Biology Vol. 13 No. 2 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.02.011

Abstract

The genus Tor (Cyprinidae) comprises highly diverse freshwater fishes widely distributed in Southeast Asia, including Indonesia. However, information on Tor species in several regions remains limited. This study aimed to identify Tor species from Telaga Rambut Monte, Blitar, using morphological analysis and molecular approaches based on COI DNA barcoding. Fish were collected through purposive sampling at depths of 1–2 m, and fin tissues were preserved in ethanol for DNA extraction. PCR amplification employed LCO1490 and HCO2198 primers, with sequence analysis conducted using BLAST and the BOLD System. Phylogenetic reconstruction applied Neighbour-Joining and Maximum Likelihood methods under the Kimura 2-Parameter model, supported by p-distance analysis with MEGA, Clustal X, BioEdit, and ABGD. Morphological characterisation confirmed the specimens as Tor tambra. COI gene amplification produced a 477 bp sequence, which showed 92% genetic similarity to the reference T. tambra. The phylogenetic tree revealed a distinct clade with a high bootstrap value (96%), clearly separating T. tambra from other congeners. Integrating morphological and molecular data provides robust evidence confirming the presence of T. tambra in Telaga Rambut Monte. This research represents the first study combining genetic identification, morphological analysis, and phylogenetic reconstruction of T. tambra from this locality. The findings contribute to understanding freshwater fish biodiversity in Indonesia and provide important baseline information on the distribution of local fish resources.
Morphology and Molecular Identification of Cyprinidae from the Sumber Umbulan Ngenep, Malang Regency, East Java Naya Rabbani, Calista; Damayanti, Ayu Elysia; Tresna Sulung, Cahya Ajeng Valenta; Rahayu, M.Si, Dwi Anggorowati; Nugroho, Endik Deni; Rusdianto, Rusdianto; Binti Mamat, Noorhidayah
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 2 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n2.p142-159

Abstract

The genus Tor comprises freshwater fish belonging to the Cyprinidae family, recognized for their remarkable diversity and broad distribution across Southeast Asia, including Indonesia. Despite this, data on Tor species in certain regions are limited, highlighting the need for additional research to confirm their presence. Therefore, this study aims to examine the genetic diversity and phylogenetic reconstruction of the species observed in Sumber Umbulan Ngenep using the COI gene as a DNA barcode marker. The research was conducting using the Purposive Random Sampling method and fin tissues were preserved in 96% ethanol for DNA analysis. The species found at Sumber Umbulan Ngenep were observed morphologically using a phenetic approach. Fins from each fish sample were collected and subjected to PCR, sequence analysis using the BOLD System, phylogenetic tree reconstruction, ABGD analysis, and QR barcoding. The results of morphological, genetic, and phylogenetic tree reconstruction based on the COI gene indicate that three species have been identified at Sumber Umbulan Ngenep, namely Tor douronensis, Tor tambra, and Barbonymus gonionotus. Based on the phenetic approach, three apomorphies and five automorphies were identified, indicating the relatedness of the observed species with the reference species. The phylogenetic tree topology shows an unambiguous branching pattern for the observed sample cluster with Poecilia reticulata as the outgroup. This study is the first to identify the species present at Sumber Umbulan Ngenep, Malang Regency, East Java, Indonesia. The findings provide new information about the existence of these species and can serve as a basis for future conservation and management strategies.
Co-Authors Binti Mamat, Noorhidayah Dwi Anggorowati Rahayu Endik Deni Nugroho Mamat, Noorhidayah Binti Naya Rabbani, Calista Rabbani, Calista Naya Rahayu, M.Si, Dwi Anggorowati Rusdianto Rusdianto, Rusdianto Sulung, Cahya Ajeng Valenta Tresna Tresna Sulung, Cahya Ajeng Valenta