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All Journal Biology, Medicine, & Natural Product Chemistry Journal of Aquaculture and Fish Health JURNAL SAINS DAN TEKNOLOGI PERIKANAN (JIKAN)
Alim, Muhammad Bachrun
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Environmental Science Medicine & Pharmacology Public Health Social Sciences

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Isolation, Identification, and Evaluation of Antimicrobial of the LAB from Bekasam: The Traditional Fermented Fish in Indonesia Rohman, Nur; Alim, Muhammad Bachrun
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.897-903

Abstract

Lactic acid bacteria (LAB) are frequently utilized in fermented foods and can incresed shelf life for the products through their secondary metabolites includes carbon dioxide, hydrogen peroxide, lactic acid and bacteriocins. Beside increasing the shelf life, LAB also affect taste, smelt, and texture. Thus, this study aimed to isolate, identify and evaluate antibacterial LAB strains from Indonesian traditional fermented fish (Bekasam). Gram staining, the catalase assay, and motility assays were used to initially characterize the presumed isolates phenotypically following primary isolation on De Man, Rogosa, and Sharper (MRS) agar. 14 isloates were determined to be presumed LAB by preliminary phenotypic testing. One species that has the highest antibacterial activity is confirmed by a 16S rRNA sequencing study. Lactobacillus plantarum CP-134 was identified from the bekasam. In the biochemical characterization all isolates were catalase-negative and non-motile. According to our research, these LAB strains may have applications in fermented foods due to their probiotic properties, which include antibacterial activity. They might be used as natural substitutes for additives and antibiotics, but more in vivo or in silico research is required to verify their potential and effectiveness. A typical traditional food preservation technique for enhancing shelf life, food safety and nutrition and sensory qualities is fermentation. Bekasam is a traditional fermented food from Indonesia.
Morphometric and Meristic Characteristics of Freckled Goatfish (Upeneus tragula) from Madura Strait, East Java Santanumurti, Muhammad Browijoyo; Hartono, Sugeng; Alim, Muhammad Bachrun
Jurnal Sains dan Teknologi Perikanan Vol 5 No 2 (2025): OKTOBER
Publisher : Fisheries Science Study Program, Faculty of Science and Technology, Muhammadiyah University of Sidenreng Rappang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55678/jikan.v5i2.2306

Abstract

The kuniran fish or goatfish, from genus Upeneus, is the most commonly found species in the world from the Mullidae family. This study aims to provide information regarding the morphological characteristics of Upeneus from the Madura Strait. The method used in this study is the survey method. This study was conducted in June 2023 in the Madura Strait (East Java, Indonesia). The parameters tested for the morphological identification of Upeneus fish are morphometric and meristic tests. Fish morphometric tests are quantitative measurements and analyses of the shape and size of fish body parts to identify fish species in terms of length or weight. Meanwhile, meristic tests are recording and analyzing the number of fish body structures that can be counted, such as the number of scales, fin rays, or certain bones. The results show that the kuniran fish/goatfish or Upeneus found in the Madura Strait was U. tragula which had the main morphometric characteristics of 17 cm SL, with a head length of 25.88, a head depth of 18.82, and a snout length of 5.88. This fish had two dorsal fins, one ventral fin, two pectoral fins, one anal fin, and a caudal fin. The ventral and anal fins were yellow, with a brownish-white caudal fin. In this study, U. tragula had meristic characteristics of VIII dorsal fin spines, 10 dorsal fin rays, 34 caudal fin rays, III + 6 anal fin rays, and 12 pectoral fin rays.
The Effect of Different Natural Extenders in Maintaining the Quality of Sperm Fish (Cyprinus carpio) Faqih, Abd Raheem; Supriatin, Febriyani Eka; Rahmawati, Aulia; Anitasari, Septi; Maulana, Gilang Drajat; Alim, Muhammad Bachrun
Journal of Aquaculture and Fish Health Vol. 13 No. 1 (2024): JAFH Vol. 13 No. 1 February 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i1.30596

Abstract

High demand on   as consume fish nor as decorative fish, causes an increasing production. But, an obstacle occurs on production, due to reproductive characteristic of Common Carp fish. Different time on sperm and eggs production from male and female fish cause difficulties in breeding. Hence, it needed sperm preservation so the breeding could be done in whole year. Natural extenders are the solution to preserve fish sperm without side effect. The purpose of this study was to determine the effect of different types of extenders and different doses on the percentage of sperm motility and viability of carp (Cyprinus carpio) after storage This research was conducted at the Faculty of Fisheries and Marine Sciences, Universitas Brawijaya and the Freshwater Cultivation Installation (IBAT Punten), Batu, East Java. The method in this study was Complete Random Design (CRD) with 6 treatments, K (100ml lactate ringer); A (1ml cider + 99ml lactate ringer); B (1ml coconut + 99ml lactate ringer); C (1ml date juice + 99ml lactate ringer); D (1ml honey + 99ml lactate ringer); E (1ml sugar cane + 99ml lactate ringer). The results showed that the highest motility was obtained in the treatment of using date extract extender with a motility percentage rate of 77.66%; highest viability was obtained in the honey extract with 80.71%; and the highest fertility rate was obtained in the honey extract treatment with 72.67 %.
Co-Authors Aulia Rahmawati, Aulia Faqih, Abd Raheem Maulana, Gilang Drajat Nur Rohman Santanumurti, Muhammad Browijoyo Septi Anitasari, Septi SUGENG HARTONO, SUGENG Supriatin, Febriyani Eka