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Laili, Alvina Nur
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Poiploidy induction of Indonesian Black Rice Oryza sativa L. Var. Cempo Ireng with Bio-catharantine Kurniawan, Ludfi; Laili, Alvina Nur; Angraeni, Devi Silvia; ‘Ain, Salsabila Qurrotu; Wulandari, Dyah Retno; Ulum, Fuad Bahrul
Life Science and Biotechnology Vol. 1 No. 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.43753

Abstract

High demand on black rice Oryza sativa L. due to their high antioxydant content and better nutrition than the white rice. On the other hand this high price rice still less interest for the farmet to cultivate it. Therefore, improvement of plant characters was carried out through polyploid induction using the natural anti-mitotic compound bio-catharanthine. The purpose of this study was to test the effectiveness of bio-catharanthine in inducing polyploidy in Cemp ireng black rice. This study used a two-factor completely randomized design (CRD) method with three replications. The treatment factor of bio-catharanthine concentration was 1%, 1.5 %, 2 %, 2 %, 2.5 % and 3 % with the soaking time of 48 hours. The germination rate, ploidy level, stomatal size and density, and antioxidant were measuret to observed the characteristic of the mutan. The results showed that the treatments of 3 % bio-catharanthine enhanced the chromosome number of black rice Cempo ireng. Biocatharantine did not affected the germination rate through the treatments. Out mutants shows alternation on the stomatal size and density. The antioxidant of leaf sample did not changed after the treatment. Bio-catharanthine on high concentration migh be possible to be applied on polyploidy induction in black rice
Characterization of Apyrase from the Salivary Glands of Ae. aegypti Based on Bioinformatics Database Wathon, Syubbanul -; Laili, Alvina Nur; Iflaha, Adriana; Widyadana, Kaysha Rahmadita; Zahniar, Zahniar; Saraswati, Dyah Rahmadhani; Oktarianti, Rike; Senjarini, Kartika
Life Science and Biotechnology Vol. 2 No. 1 (2024): May 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i1.48353

Abstract

The salivary glands of mosquitoes, which act as disease vectors, contain biological components that facilitate the blood-feeding process and transmit pathogens to the human body. Apyrase, a protein from the salivary glands of Ae. aegypti, is known to be an immunogenic protein that influences the host's immunity. It can prevent platelet aggregation by hydrolyzing ATP or ADP into AMP and thus facilitate the blood-feeding process. This mechanism is also exploited by pathogens to obtain entry into the host's body. Vaccination with the apyrase protein could control dengue virus transmission by enhancing the immune response to inhibit the pathogen's entry. To examine the potential of apyrase, it is critical to explore its nature and characteristics by using bioinformatics databases. This quantitative descriptive study involved apyrase protein from the salivary glands of Ae. aegypti obtained from several bioinformatics databases, namely UniProt, SWISSMODEL, ENZYME, Rhea, CFSSP, GlyCosmos, STRING, and QuickGo. The findings contribute to a more fine-cut understanding of apyrase as a potential vaccine to inhibit dengue transmission.
Co-Authors Angraeni, Devi Silvia ‘Ain, Salsabila Qurrotu Fuad Bahrul Ulum Iflaha, Adriana Kartika Senjarini Kurniawan, Ludfi Rike Oktarianti Saraswati, Dyah Rahmadhani Wathon, Syubbanul - Widyadana, Kaysha Rahmadita Wulandari, Dyah Retno Zahniar, Zahniar