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All Journal Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Kedokteran Hewan Jurnal Medika Veterinaria

Rahmat Setya Adji

Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Author-ID : 220034

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Published : 5 Documents Claim Missing Document

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Articles

The Control of Anthrax Disease: Diagnosis, Vaccination and Investigation Adji, Rahmat Setya; Natalia, Lily
Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Anthrax is a bacterial disease caused by Bacillus anthracis attacking both animal and human (zoonosis) . The disease is normally associated with domestic livestock such as sheep, goats, and cattle, but humans are also infected due to exposure or comsuming infected animals . The control of anthrax in humans and animals involves developing a diagnostic method for B. anthracis detection and confirmation of anthrax, prevention by vaccines, and disease investigation . Rapid and more accurate diagnosis techniques for anthrax should be developed for improving the conventional method used in Indonesia . Vaccines are effective against anthrax . Current anthrax vaccine used in Indonesia is spores vaccine produced from a non-encapsulated, toxigenic. Sterne strain 34F2 of B. anthracis . The use of this vaccine occasionally causes local pain, necroses at the inoculation site, subcutaneous oedema and occasionally death of the animal . Several vaccines have been developed recently such as sub unit vaccine, anthrax vaccine absorbed (AVA), that contains a protective antigen (PA) component of the anthrax toxin as the major protective immunogen and is usually used in humans. In endemic areas of anthrax, outbreaks still routinely occur almost yearly . Monitoring of the epidemiological patterns of the disease has to be carried out by field investigation . Key words: Anthrax, Bacillus anthracis, zoonotic disease, disease control

Confirmation test of suspected Mycobacterium avium subspecies paratuberculosis (MAP) isolated using PCR F57 Nugroho, Widagdo Sri; Adji, Rahmat Setya; Wahyuni, Aeth
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 2 (2008)
Publisher : Indonesian Animal Sciences Society

Seropositive and isolate suspected as Mycobacterium avium subspecies paratuberculosis (MAP) was detected at dairy cows in West Java. This bacteria causes Johneâs disease (JD) and potentially becomes a new emerging disease for Indonesian dairy cows. The aim of this study was to confirm the suspected local isolate as a MAP distinctively by PCR. Reculture of MAP reference isolate, suspected local isolate done by resuspending bacteria in PBS 0.5% and inoculating it in Herroldâs egg yolk medium with mycobactin J (HEYM) and than inoculating it in 37oC for 16 weeks. The cultures grew in various time, Mycobacterium avium subspecies avium was detected in 3rd week, MAP reference was detected in 7th week, and local isolate was detected in 14th week. The confirmation test was carried out by PCR with primer F57. The PCR F57 result showed that MAP suspected isolate was not a Mycobacterium avium subspecies paratuberculosis. Key Words: Local Isolate, Mycobacterium avium Subspecies Paratuberulosis, PCR F57

Rapid identification of Bacillus anthracis by cell wall and capsule components direct fluorescent antibody assay Natalia, Lily; Adji, Rahmat Setya
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 2 (2008)
Publisher : Indonesian Animal Sciences Society

During the outbreak of anthrax, early diagnosis is critical for effective treatment. Numerous attempts have been made to design antigen based detection tests and to rapidly identify truly anthrax specific antigens for B. anthracis. In Indonesia, standard identification of B. anthracis relies on a combination of time consuming steps including bacterial culture and Ascoli precipitin test, which can take several days to provide a diagnosis. In this study, two component (cell wall and capsule) direct fluorescent antibody assay (DFA) were developed to rapidly identify and to directly detect capsulated B. anthracis. The component used in cell wall DFA (CW-DFA) assay is polysaccharide-peptidoglycan complex, which was prepared from B. anthracis culture by cell lysis, guanidine and sodium dodecyl sulphate (SDS) extraction. The component used in capsule DFA (CAP-DFA) is poly-D-glutamic acid (PGA) which were prepared by extraction of B. anthracis capsule. Component of polysaccharide-peptidoglycan complex and PGA conjugated with hemocyanin were then used as immunogen for immunizing rabbits using Freundâs complete/incomplete adjuvant. The hyperimmune sera were then collected, purified and conjugated to Fluorecent Iso Thiocyanate (FITC). B. anthracis isolates and non B. anthracis isolates were tested by the CW-DFA and CAP-DFA Assays. B. cereus, B. subtilis, other Bacillus sp. and other Gram positive rod bacteria were negative, while capsulated B anthracis gave positive results. The two component (CW DFA and CAP-DFA) assay are specific rapid confirmatory test for capsulated B. anthracis. Key Words: Bacillus anthracis, Cell Wall and Capsule Direct Fluorescent Antibody Assay

Detection of Very Virulent Infectious Bursal Disease (IBD) in Chicken in West Java Putri, Ajeng Fabeana; Winarsongko, Agus; Hoerudin, Heri; sekarmila, Gita; Ahpas, Ahpas; Jaelani, Jejen; Gunawan, Wawan; Dewiyanti, Rina; Pratama, Yuda; Nuradji, Harimurti; Fairusya, Nuha; Ekawasti, Fitrine; Adji, Rahmat Setya; Dharmayanti, NLP Indi; Indriani, Risa; Utomo, Bambang Ngaji; Suryatmiati, Sri
Jurnal Medika Veterinaria Vol 18, No 1 (2024): J.Med.Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v18i1.35819

Infectious Bursal Disease (IBD), also known as Gumboro disease, is an acute, highly contagious disease that infects chickens and causes a high mortality rate of up to 100% in young animals. The disease is caused by Infectious Bursal Disease Virus (IBDV) of the genus Avibirnavirus, family Birnaviridae. The disease has been reported in Indonesia since 1976, and management strategies for the disease, such as vaccination, have been applied to prevent and control outbreaks in poultry farms. In this study, we conducted the detection of the disease in chickens from a farm in West Java with a mortality rate of 80%. Chickens showing clinical signs, such as sudden death, anorexia, watery diarrhea, and ruffled feathers, were necropsied, and organ samples, including the bursa Fabricius, brain, and spleen, were collected. The samples were then tested using Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) to confirm the diagnosis of IBD. Positive results were obtained in this study, highlighting the need for improved biosecurity in poultry farms in Indonesia. These results also provided a basis for further research on viral characterization to develop detection kits or vaccines for IBD using local isolates from the field in Indonesia.

EVALUASI STATUS VIRULENSI ISOLAT Bacillus anthracis ASALNUSA TENGGARA DAN PAPUA MENGGUNAKAN METODE POLYMERASE CHAIN REACTION MULTIPLEX Ebenhaizar Sanam, Maxs Urias; Asmara, Widya; Tri Hastuti Wahyuni, Agnesia Endang; Wibowo, Michael Haryadi; Adji, Rahmat Setya
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2802

Penelitian ini bertujuan mengevaluasi status virulensi 22 isolat Bacillus anthracis (B. anthracis) asal Nusa Tenggara dan Papua menggunakan metode polymerase chain reaction (PCR) multiplex dengan dua pasang primer nukleotida yang memiliki target amplifikasi gen spesifik pada kedua plasmid. Ektraksi DNA dilakukan dengan metode lisis panas. Pasangan primer PA5 dan PA8 digunakan untuk mengamplifikasi gen pagA pada pXO1, sedangkan pasangan primer 1234 F dan 1301 R mengamplifikasi gen capABC pada pXO2. Hasil reaksi PCR menghasilkan dua pita DNA berukuran sekitar 600 dan 800 bp pada 20 isolat. Namun, dua isolat lain, masing-masing hanya memiliki salah satu dari kedua ukuran pita DNA tersebut. Sebagian besar koleksi isolat asal Nusa Tenggara dan Papua (91%) masih memiliki kedua plasmid secara lengkap (pXO1+/2+) dan karena itu bersifat virulen, sedangkan dua isolat lain (9%) telah kehilangan salah satu plasmid virulennya sehingga bersifat avirulen. Disimpulkan bahwa PCR multiplex dengan dua pasang primer dengan target amplifikasi pada plasmid dapat digunakan untuk evaluasi status virulensi isolat B. anthraci.

Co-Authors Agnesia Endang Tri Hastuti Wahyuni Ahpas, Ahpas Bambang Ngaji Utomo Dewiyanti, Rina Fairusya, Nuha Fitrine Ekawasti, Fitrine Hoerudin, Heri Jejen Jaelani Lily Natalia Maxs Urias Ebenheizer Sanam Michael Haryadi Wibowo NLP Indi Dharmayanti Nuradji, Harimurti Putri, Ajeng Fabeana RISA INDRIANI sekarmila, Gita Suryatmiati, Sri Wawan Gunawan Widagdo Sri Nugroho Widya Asmara Winarsongko, Agus yuda pratama

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