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All Journal Narra J Jurnal Farmasi Galenika
Larasti, Veny
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Isolasi Dan Karakterisasi Bromelain Bonggol Nanas Dari Limbah Industri (Ananas comosus L. Merr.) Mulyani, Laida Neti; Larasti, Veny; Handayani, Dwi; Herlina, Herlina
JURNAL FARMASI GALENIKA Vol 11 No 1 (2024): Jurnal Farmasi Galenika Vol 11 No 1
Publisher : Universitas Bhakti Kencana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70410/jfg.v11i1.328

Abstract

Waste of pineapple stem (Ananas comusus L. Merr.) has not been optimally utilized, although it is known that pineapple contain bromelain enzym. This research aimed to isolation and characterization bromelain from pineapple stem. Enzim was extracted from pineapple stem to produce crude enzim of bromelain. Isolation bromelain was done by amonium sulphate precepititation and continued by dialysis. Characterization to determined molecular weight, protein content and protease activity. The result of fractionation by amonium precipitation showed the highest specific activity on amonium sulphate 40-60% fraction 1011,98 U/mg. Caharacterization of purified bromelain showed that molecular weight of bromelain is 23,69 dan 24,72 kD with proteint content 2,78 mg/mL and protease activity is 2833,33 U/mL. This study concluded that the pineapple stem has bromelain enzym with the protease activity.
Comparison of anti-SARS-CoV-2 IgG seropositivity among vaccinated, unvaccinated, and COVID-19 survivor individuals: A cross-sectional study in Palembang, Indonesia Retnaningsih, Ekowati; Nuryanto, Nuryanto; Oktarina, Reni; Komalasari, Oom; Maryani, Sri; Larasti, Veny; Fertilita, Soilia
Narra J Vol. 5 No. 3 (2025): December 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i3.2890

Abstract

Measurement of anti–severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin G (IgG) antibody levels provides evidence of prior infection or vaccination. Persistent vaccine hesitancy underscores the importance of robust, evidence-based data to inform policy decisions. The aim of this study was to compare anti-SARS-CoV-2 IgG seropositivity among vaccinated individuals, unvaccinated individuals, and coronavirus disease 2019 (COVID-19) survivors in the community during the pandemic. In this cross-sectional study, 517 participants were enrolled, including 167 vaccinated individuals, 97 COVID-19 survivors, and 253 unvaccinated individuals, selected through multistage cluster sampling of 40 clusters. Anti-SARS-CoV-2 IgG seropositivity was defined as ≥50 AU/mL. Multivariable logistic regression was performed to evaluate associations between group type and seropositivity, adjusting for demographic factors, COVID-19 symptoms, hypertension, and body mass index (BMI). Vaccinated individuals demonstrated significantly higher odds of seropositivity compared with unvaccinated participants (odds ratio (OR)=5.60; 95% confidence interval (CI): 2.36–13.27). Covariates independently associated with seropositivity (p<0.05) included the presence of COVID-19 symptoms, hypertension, and BMI. Vaccination was strongly associated with increased anti-SARS-CoV-2 IgG seropositivity in the community, independent of clinical and demographic factors. These findings support ongoing vaccination campaigns and highlight the relevance of comorbidities and symptomatic history in shaping humoral immune responses.
Co-Authors Dwi Handayani Ekowati Retnaningsih Fertilita, Soilia Herlina Herlina Maryani, Sri Mulyani, Laida Neti Nuryanto Nuryanto Oom Komalasari Reni Oktarina