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All Journal Jurnal EduHealth Journal of Pharmaceutical and Sciences.
Mierza , Vriezka
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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Standardization of Anthocyanin Compounds in Plants in Indonesia: Literature Review Agustin, Annastya Eka; Mierza , Vriezka; Annisa, Bunga Nur; Sa’adah, Cantika Nurul; Prasetyo, Fajar; Lubis, Christina Febiola
Jurnal EduHealth Vol. 13 No. 02 (2022): Jurnal eduHealth, Periode Oktober - December, 2022
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.134 KB)

Abstract

Anthocyanins are secondary metabolites belonging to the flavonoid group which act as pigments or coloring agents in plants that give red, blue and purple colors to flowers, fruits and vegetables. Anthocyanins have several pharmacological activities such as antioxidants, anti-carcinogenic, preventing impaired liver function, anti-hypertensive, and anti-diabetic. The anthocyanin structure consists of an aglycone form which is sterified by several sugar groups (glycones) and acyl groups in several anthocyanin compounds. In an effort to maintain the standard of a drug made from natural ingredients, the process of standardization of active compounds can be carried out to guarantee and maintain safety, quality uniformity and efficacy. This review was conducted to identify the anthocyanins contained in an extract by means of thin layer chromatography (TLC), UV-Vis spectrophotometer, thin layer chromatography-densitometry, and high performance liquid chromatography (HPLC). The research methodology used is in the form of literature review with reference sources related to the review topic obtained from various sources, such as Pubmed, Science Direct, and Google Scholar.
The Literature review: Investigasi Perbandingan Antioksidan pada Tanaman Obat Herbal di Pulau Jawa dengan Spektrofotometri UV-Vis dan Metode DPPH (2,2-difenil-1-pikrilhidrazil) Sebayang , Madame Marselly; Mierza , Vriezka
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.554

Abstract

Due to environmental changes and pollution due to free radicals, interest in alternative medicine is increasing, especially on the island of Java. Natural health solutions are increasingly needed, which is what prompted this research. We measured the concentration of antioxidant compounds using the UV-Vis spectrophotometric method and the free radical scavenging capacity of plant extracts using the DPPH method. Using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and UV-Vis spectrophotometric methods with IC50 value determination, this article aims to investigate and compare potential herbal plants found on the island of Java. This research was carried out using a literature review method by considering inclusion and exclusion criteria with keywords such as antioxidants, herbal medicinal plants, UV-Vis spectrophotometry, and DPPH method. The results showed that the plant species studied had different antioxidant activities. Some herbs have high levels of antioxidants, so they may be used as natural health supplements. Additionally, this study emphasizes how important it is to use accurate analytical methods to evaluate antioxidant content. To provide a comprehensive picture of the antioxidant potential of each plant, UV-Vis spectrophotometry and DPPH methods work together. The results show that this study identified several herbal plants on the island of Java that have high levels of antioxidants. These plants can be used to develop herbal-based health products. To carry out a comparative evaluation of antioxidant content, the UV-Vis and DPPH spectrophotometric methods were proven to be effective. This discovery can be the basis for further research and development of more effective and useful herbal health products.
Co-Authors Agustin, Annastya Eka Annisa, Bunga Nur Lubis, Christina Febiola Prasetyo, Fajar Sa’adah, Cantika Nurul Sebayang , Madame Marselly