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All Journal Denta: Jurnal Kedokteran Gigi
Istien Wardani
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Dentistry

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The Antibacterial Effect of Anchovy (Stolephorus Insularis) Extract Against Enterococcus Faecalis Ilham Mahendra; Istien Wardani; Linda Rochyani
Denta Journal Kedokteran Gigi Vol 12 No 2 (2018): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Backgrounds: Enterococcus faecalis is a facultative anaerobic gram-positive bacteria that known to have a role in the infection of the root canal, causing the failure of root canal treatment. The extract from anchovy (Stolephorus insularis) contains fluor has antibacterial properties that can inhibit the growth of gram-positive bacteria and has the potential to be developed as a root canal sterilization agent. Purpose : To determine the antibacterial effect of anchovy (Stolephorus insularis) extract to the Enterococcus faecalis. Methods : This study was an true experimental study with post test only control group design and were tested by diffusion methods, consisted of 5 groups: 2 control groups DMSO 1% as negative control and ChKM as positive control, 3 treatment groups of anchovy (Stolephorus insularis) extract with different concentrations of 18%, 24% and 30% which each group consisted of 5 samples. Antivbacterial effect was examined by measuring the clear zone around the filter paper. Data were analyzed by ANOVA test followed by LSD test. Results : Results of this study proved the presence of antibacterial effects of anchovy (Stolephorus insularis) extract to the Enterococcus faecalis. In this study, the higher concentrations used, the greater the antibacterial effect. Average inhibition zone of each groups: DMSO 1% (6,03 mm), ChKM (14,18 mm), 18%(6,83 mm), 24%(7,03 mm) and 30% (7,16 mm). Conclusion : Anchovy (Stolephorus insularis) extract has antibacterial effect to the Enterococcus faecalis.
The Effectiveness Of Anchovy Concentration (Stolephorus insularis) as Antimicrobial to Streptococcus mutans (In Vitro) Almira Fa’Izah; Istien Wardani; Diana Soesilo
Denta Journal Kedokteran Gigi Vol 10 No 1 (2016): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: Dental and oral diseases which are often found in children is dental caries. Streptococcus mutans is the main cause of caries. Caries can be prevented by using a topical application of fluoride. The Anchovy (Stolephorus insularis) contains protein, vitamins (A, B1, C), and minerals (Fe, Ca, K, F). Calcium fluoride (CaF2) within the anchovy can inhibit the occurrence of dental caries. Purpose: The aim of this study was to determine the antimicrobial ability of anchovy extract (Stolephorus insularis) to Streptococcus mutans. Materials and Methods: This study was a laboratory experimental research with post test only control group design. Diffusion method were applied with 2 controls: negative control used DMSO 1%, positive control used NaF solution, and 3 concentrations of anchovy extract (Stolephorus insularis) 3%, 6%, and 12%, each group were composed of 6 samples. Antimicrobial was assessed by measuring the diameter of the clear zone around the discs contained the anchovy extract (Stolephorus insularis). Data were analyzed by Kruskal-Wallis test followed by Mann-Whitney test. Result: The results from this study showed clear zone around the discs of the anchovy extract (Stolephorus insularis). The more concentration of the extract showed the more antimirobial zone diameter. The average zone of antimicrobial at the concentration of 3% were 7,11 mm, 6% 9,5 mm, 12% 10,78 mm, for the negative control DMSO 1% 6 mm and the positive control NaF solution 8,16 mm. The largest diameter of the clear zone was at concentration of 12% (P < 0,05). Conclusion: The anchovy extract (Stolephorus insularis) had an antimicrobial effect to the growth of Streptococcus mutans.
The antibacterial effect of Anchovy (Stolephorusinsularis) Extract Against P.aeruginosa Istien Wardani; Annisa Listya
Denta Journal Kedokteran Gigi Vol 14 No 1 (2020): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v14i1.5

Abstract

Introduction: Root canal infection is largely a continuation of the caries process that is not treated and develops so that it involves the root canal. Sterilization is a part of multivisit root canal treatment and important for the success of root canal treatment. The Anchovy (Stolephorus insularis) contains protein, vitamins (A, B1, C), and minerals (Fe, Ca, K, F). Calcium fluoride (CaF2) contained in anchovies can inhibit bacteria that cannot be removed by chemo-mechanical processes such as instrumentation and irrigation Purpose: The aim of this study was to determine the antimicrobial ability of anchovy extract (Stolephorus insularis) to P. aeruginosa. Materials and Methods: This study was a laboratory experimental research which with post test only control group design. Diffusion method were applied with 2 controls: positive control using ChKM solution, negative control using 1% DMSO and 3 concentrations of jengki anchovy extract (Stolephorus insularis) 18%, 24%, and 30% as treatment groups, where each group consisted of 5 samples. Antimicrobial was assessed by measuring the diameter of the clear zone around the discs contained the anchovy extract (Stolephorus insularis). Data were analyzed by Kruskal-Wallis test followed by Mann-Whitney test. Result: The results from this study showed clear zone around the discs of the anchovy extract (Stolephorus insularis). The more concentration of the extract showed the more antimicrobial zone diameter. The average inhibitory zone at 18% concentration was 6.03 mm, 24% 7.59 mm, 30% 8.69 mm, positive control of ChKM solution 31.43 mm for negative control DMSO 1% 6.03 mm. The largest diameter of the clear zone is at a concentration of 30%. Conclusion: The results obtained showed that the inhibition zone of the Stolephorus insularis extract concentration of 30% had the largest average among the other concentrations of 8.69 mm.
Efektivitas Topikal Aplikasi Fluoride Menggunakan Ekstrak Teh Hijau Dibandingkan dengan Sodium Fluoride Pada Gigi Sapi Ekky Berliana R P; Istien Wardani; Eriza Juniar
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: Prevention of caries and periodontal disease by performing dental health improvement has become a major destination in the world of dentistry. Topical application of fluoride is one of the most effective ways to prevent caries, fluoride inhibits the absorption of salivary proteins on the surface of the email so the pellicle and plaque formation reduced,increase enamel remineralization, resistance to acids and decreased pH. Topical application of fluoride commonly used is sodium fluoride 0.1%. Green tea (Camellia sinensis) can be an alternative herbs as a topical application of fluoride because it has reduced the growth of plaque and have antibacterial. Purpose: To determine the effectiveness of topical fluoride applications using green tea extract compared to sodium fluoride on the teeth cow. Materials and Methods: This study was true experimental laboratories with post test only control group design. The subjects in this study was a teeth cow (Bovine) is applied with the topical application of fluoride, control group without administration of topical application offluoride, treatment group one with Sodium fluoride 0.1%, and the treatment group two with green tea extract (Camellia sinensis) 0.1%. Counting the amount of fluoride by Energy Dispersive X-ray Spectrophotomrtry (EDS). The data was processed by Levene test and one way ANOVA. Result: There are no significant differences in the number of fluoride among all groups (P> 0.05). Conclusion: There were no significant differences in the effectiveness of topical application of fluoride using green tea extract (Camellia sinensis) concentration of 0.1% compared with 0.1% Sodium fluoride on teeth cow (Bovine.
Co-Authors Almira Fa’Izah Annisa Listya Diana Soesilo Ekky Berliana R P Eriza juniar Ilham Mahendra Linda Rochyani