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All Journal Denta: Jurnal Kedokteran Gigi
Linda Rochyani
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Dentistry

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The Inhibition of Cyanobacteria Spirulina Extract on S. Mutans Biofilm as an Ingredient in Mouthwash Henu Sumekar; Linda Rochyani; Istien Wardani
Denta Journal Kedokteran Gigi Vol 18 No 2 (2024): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v18i2.8

Abstract

Background: The main cause of dental caries is Streptococcus mutans (S. mutans), to suppress the growth of cariogenic bacteria can be done by using mouthwash containing antibacterial ingredients such as chlorhexidine. Cyanobacteria spirulina extract contains compounds that have secondary metabolites which act as antibacterial and antioxidant including tannins, flavonoids, saponins and terpenoids. Objective: This study aimed to find out he inhibition of Cyanobacteria spirulina against S. mutans biofilm. Materials and Methods: The research sample consisted of 6 groups. Group K(-) was the S. mutans biofilm with aquadest, K(+) was Chlorhexidine 0.2% and four treatment groups (P1, P2, P3, P4), which were Cyanobacteria spirulina extract with a concentration of 60 , 70, 80 and 90 mg/ml. The sample was applied to a well microtiter plate, then incubated overnight at 37°C for 1 x 24 hours, then painted with 0.1% crystal violet. After that, it was rinsed with sterile distilled water, then given 0.2 mL of Tween 80 concentration of 2% and measured Optical Density with an ELISA Reader with a wavelength of 570 nm, then biofilm inhibition percentage as calculated. Results: The mean value of the percentage of S. mutans biofilm inhibition in group K(+): -131,694(SD 10,758) ; P1: -12,234(SD 2,402) ; P2: 11,076(SD 6,387) ; P3: 19.7020(SD 11,670) ; P4: 40,214(12,057) there were significant differences between P2 and P4 also between P3 and P4. Conclusion: Cyanobacteria spirulina at concentrations of 70, 80 and 90 mg/ml had S. mutans biofilm inhibition, while Chlorhexidine 0.2% and Cyanobacteria spirulina 60 mg/ml did not.
The Difference of Surface Roughness of Heat Cured Acrylic Resin After Brushing with 30% and 60% Lemongrass Extract in Pasta Clarissa Valerie Wardojo; Paulus Budi Teguh; Linda Rochyani
Denta Journal Kedokteran Gigi Vol 13 No 1 (2019): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v13i1.178

Abstract

Background: Tooth loss can be replaced by the use of dentures. Cleaning of dentures by brushing using toothpaste can cause surface roughness because of abrasive materials. The use of toothpaste can be replaced with paste of extract Cymbopogon citratus because it has antibacterial and antifungal effects. Purpose: to determine the effect of brushing using paste of extract Cymbopogon citratus on the surface roughness of heat cured acrylic resin. Methods: Sample were heat cured acrylic resin (62.5 x 10 x 2.5) mm, divided into 4 groups consist of negative control group was brushed with basic paste, positive control group was brushed with herb paste, and two groups were brushed with paste of Cymbopogon citratus extract with 30% and 60% concentrations. Brushing was done for 3 minutes with 60 brushing times and then measured with surface roughness tester. The data was processed by ANOVA test. Result: The average surface roughness in K-, K+, P1, and P2 were 0,1217, 0,3433, 0,1633, and 0,27 μm. There was significant difference between negative control group and positive control group; negative control group with P1 and P2 group; positive control group with P1 and P2 group; P1 group with P2 group (P <0.05). Conclusion: The paste of Cymbopogon citratus extract concentration of 30% was effectively used because the level of surface roughness below the maximum limit of recommended surface roughness of dentistry material which is 0,2 μm. The 60% concentration paste of Cymbopogon citratus extract was less effective because the level of surface roughness above the maximum limit of surface roughness is recommended.
The Antibacterial Effect of Anchovy (Stolephorus Insularis) Extract Against Enterococcus Faecalis Ilham Mahendra; Istien Wardani; Linda Rochyani
Denta Journal Kedokteran Gigi Vol 12 No 2 (2018): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Backgrounds: Enterococcus faecalis is a facultative anaerobic gram-positive bacteria that known to have a role in the infection of the root canal, causing the failure of root canal treatment. The extract from anchovy (Stolephorus insularis) contains fluor has antibacterial properties that can inhibit the growth of gram-positive bacteria and has the potential to be developed as a root canal sterilization agent. Purpose : To determine the antibacterial effect of anchovy (Stolephorus insularis) extract to the Enterococcus faecalis. Methods : This study was an true experimental study with post test only control group design and were tested by diffusion methods, consisted of 5 groups: 2 control groups DMSO 1% as negative control and ChKM as positive control, 3 treatment groups of anchovy (Stolephorus insularis) extract with different concentrations of 18%, 24% and 30% which each group consisted of 5 samples. Antivbacterial effect was examined by measuring the clear zone around the filter paper. Data were analyzed by ANOVA test followed by LSD test. Results : Results of this study proved the presence of antibacterial effects of anchovy (Stolephorus insularis) extract to the Enterococcus faecalis. In this study, the higher concentrations used, the greater the antibacterial effect. Average inhibition zone of each groups: DMSO 1% (6,03 mm), ChKM (14,18 mm), 18%(6,83 mm), 24%(7,03 mm) and 30% (7,16 mm). Conclusion : Anchovy (Stolephorus insularis) extract has antibacterial effect to the Enterococcus faecalis.
The inhibition of leaf extract Moringaoleifera on the formation biofilm bacteria Enterococcus faecalis Linda Rochyani
Denta Journal Kedokteran Gigi Vol 14 No 1 (2020): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v14i1.7

Abstract

Background: Enterococcus faecalisis the most common bacteria that cause failure to root canal treatment, which can become very resistant under biofilms. Moringaoleifera has antibacterial properties and may affect the multidrug-resistant bacteria. Purpose: This study aimed to observe the inhibition of Moringaoleifera leaf extract on the biofilm formation of bacteria Enterococcus faecalis. Method: This study was true experimental laboratory research with post-test only control group design and tested using biofilm method, divided into six groups, each group consisted of eight samples. The control groups were: K- (CMC 0,1%), K+ (ChKM), and four treatment groups were: P1 (Moringaoleifera 20%), P2 (Moringaoleifera 40%), P3 (Moringaoleifera 60%), P4 (Moringaoleifera 80%). Antibacterial inhibition was determined by the value of Optical Density in the ELISA Reader. Data analysis using Kruskal-wallis followed by Mann-Whitney test. Results: There were significant differences (p <0.05) seen from the percentage value of biofilm inhibition, on the K - (0 %) group compared with K+ (47,69%), P1(7,68%), P2 (21,13%), P3 (42,33%) and P4 (55,78%), as well on K + group (ChKM) compared with P4 group (Moringaoleifera 80%). Conclusion: Moringaoleiferaleaf extract has inhibition effect for the formation of bacteria Enterococcus faecalis biofilm and the effect is 80% greater than ChKM.
Daya Hambat Ekstrak Teripang Emas (Stichopus hermanii) terhadap Bakteri Enterococcus faecalis Roy Tamara; Linda Rochyani; Paulus Budi Teguh
Denta Journal Kedokteran Gigi Vol 9 No 1 (2015): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: Enterococcus faecalis is one caused bacteria of root canal infections. ChKM is mostly used as sterilization agent in endodontic treatment. Stichopus hermanii extract has been reported to have antibacterial effects for gram-negative and gram-positive bacteria, so could be potentially developed as a root canal sterilization agent. Purpose: The aim of this study was to determine the inhibitory effect of Stichopus hermanii extract on Enterococcus faecalis bacteria. Materials and Methods: This study was an experimental study with post test only control group design and were tested by diffusion methods with 4 groups concentration of 2,5%, 3%, 3,5%, 4%, and 2 controls groups using DMSO 1% as negative control, and ChKM as positive control, each group consisted of 7 samples. The inhibition effect were examined by measure the diameter of the clear zone around the disc. Data were analyzed by one way ANOVA test and followed by LSD test. Results: Results showed that the mean of inhibition zone at concentrations of 2,5% (7,3543 mm), 3% (8,1086 mm), 3,5% (8,7286 mm), 4% (9,4029 mm), DMSO 1% (6,0257 mm) and ChKM (25,49 mm). It had been proved that. Stichopus hermanii extract could inhibit the growth of Enterococcus faecalis (p<0,05). The largest diameter of the clear zone was in the concentration of 4%. Conclusion: Stichopus hermanii extract could inhibit the growth of Enterococcus faecalis and the most effective inhibitory concentration is 4%.
Co-Authors Clarissa Valerie Wardojo Henu Sumekar Ilham Mahendra Istien Wardani Paulus Budi Teguh Roy Tamara