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All Journal Denta: Jurnal Kedokteran Gigi JPMP
Henu Sumekar
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Humanities Dentistry Engineering Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences

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EFEKTIVITAS EDUKASI KESEHATAN GIGI DENGAN METODE TRAIN THE TRAINERS DALAM MENINGKATKAN PENGETAHUAN GURU DAN WALI MURID SD YAPITA SURABAYA Andriani, Dwi; Twi Agnita Cevanti; Nora Lelyana; Aprilia; Dianty Saptaswari; Bambang Sucahyo; Dwi Setianingtyas; Nafiah; Henu Sumekar; Arya Brahmanta; Shintya Rizki Ayu Agitha; Sularsih; Yufita Fitriani; Vivin Ariestania; Arifzan Razak
Jurnal Pengabdian Masyarakat Pesisir VOLUME 4 NOMOR 1
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v4i1.137

Abstract

Dental and oral problems in Indonesian children are caused by low knowledge, comprehension, and behavior that ignores dental and oral health maintenance, resulting in tooth and mouth damage. This activity is in partnership with SD Yapita which is a school supported by the Faculty of Dentistry, Hang Tuah University. The aim is to prepare parents and teachers of Yapita Elementary School students to become ambassadors for oral and dental hygiene so that they can become mentors for their children or students. This method is carried out online and offline from classes 1 to 6 using a simple random sampling technique. The results were statistically analyzed using the Paired T-test. The average pre-test score from the teacher was 60 and the parents were 56, while the post-test result from the teacher was 84 and the parents was 85. The results of the data analysis were normally distributed (p>0.05) followed by the Paired correlation test T-test. The results show a relationship between the pre and post-test of teachers and parents. This shows that this educational activity can increase the knowledge of teachers and parents so that they can become ambassadors of oral and dental hygiene to their children or students.
The Inhibition of Cyanobacteria Spirulina Extract on S. Mutans Biofilm as an Ingredient in Mouthwash Henu Sumekar; Linda Rochyani; Istien Wardani
Denta Journal Kedokteran Gigi Vol 18 No 2 (2024): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v18i2.8

Abstract

Background: The main cause of dental caries is Streptococcus mutans (S. mutans), to suppress the growth of cariogenic bacteria can be done by using mouthwash containing antibacterial ingredients such as chlorhexidine. Cyanobacteria spirulina extract contains compounds that have secondary metabolites which act as antibacterial and antioxidant including tannins, flavonoids, saponins and terpenoids. Objective: This study aimed to find out he inhibition of Cyanobacteria spirulina against S. mutans biofilm. Materials and Methods: The research sample consisted of 6 groups. Group K(-) was the S. mutans biofilm with aquadest, K(+) was Chlorhexidine 0.2% and four treatment groups (P1, P2, P3, P4), which were Cyanobacteria spirulina extract with a concentration of 60 , 70, 80 and 90 mg/ml. The sample was applied to a well microtiter plate, then incubated overnight at 37°C for 1 x 24 hours, then painted with 0.1% crystal violet. After that, it was rinsed with sterile distilled water, then given 0.2 mL of Tween 80 concentration of 2% and measured Optical Density with an ELISA Reader with a wavelength of 570 nm, then biofilm inhibition percentage as calculated. Results: The mean value of the percentage of S. mutans biofilm inhibition in group K(+): -131,694(SD 10,758) ; P1: -12,234(SD 2,402) ; P2: 11,076(SD 6,387) ; P3: 19.7020(SD 11,670) ; P4: 40,214(12,057) there were significant differences between P2 and P4 also between P3 and P4. Conclusion: Cyanobacteria spirulina at concentrations of 70, 80 and 90 mg/ml had S. mutans biofilm inhibition, while Chlorhexidine 0.2% and Cyanobacteria spirulina 60 mg/ml did not.
The Cytotoxicity of Daruju Mangrove (Acanthus ilicifolius) Leaf Extract as Root Canal Irrigation Ratna Putri; Twi Agnita Cevanti; Henu Sumekar
Denta Journal Kedokteran Gigi Vol 12 No 1 (2018): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: The cleaning and shaping of the root canal are important stages in root canal treatment. The irrigation solution needed in those stages to clean the canal from residual necrotic tissue, demtine particles, and microorganism. Irrigation solution must fulfill some criteria among others, able to solute the debris or tissue residual, has low surface tension and not toxic. Mangrove Daruju (Acanthus ilicifolius) has potency to be a alternative of the usual irrigation solution because has antibacterial effect. Purpose: The aim of this study was to examine the cytotoxicity of Daruju mangrove (Acanthus ilicifolius) as root canal irrigation against the fibroblast cell (BHK-21) culture. Materials and Methods: The samples used was fibroblast cells (BHK-21) using culture method. These samples were treated with Acanthus ilicifolius chloroform extract with several concentration. Samples were divided into 1: cell control, 2:media control, 3: 40mg/ml, 4: 50mg/ml, 5: 60mg/ml, 6: 70mg/ml,: 80mg/ml, 7: control. MTT was added, 3 minutes after that incubated for 4 hours. DMSO solution was added and then shaken, the samples were analyzed using ElISA reader with a 620 wavelength. The cytotoxiciy wa expressed by cell viability. If its is > 50%, it is declared as non toxic. Data analyzed using non parametric test (Kruskal-Wallis) followed by Mann Whitney test. Results: The Kruskal-Wallis test proved that there were significant differences in the cell viability among the treated groups. The average difference among the treated groups which were tested with Mann-Whitney test, showed a significant difference between group 1 and 5, also group 2 and group 5. Conclusions: Acanthus ilicifolius leaf chloroform extract has no cytotoxicity effect on concentration fibroblast cell (BHK-21) culture.
The Secondary Metabolites Screening and the Effectiveness (Ananas comosus (L.) Merr of the Queen Pineapple Stems in Decreasing the Number of Enterococcus faecalis’s Colonies Shinta Nurmaraya Febrianti; Twi Agnita Cevanti; Henu Sumekar
Denta Journal Kedokteran Gigi Vol 10 No 1 (2016): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: A cause of endodontic treatment failure was sterilization process error so that bacterias still left in the root canal. Enterococcus faecalis were resistant to sterilization medicines, it was necessary to develop natural sterilization medicines such as the Queenpineapple stems that had the antibacterial content. Purpose: The aim of this research was to determine the effectivity of the Queen pineapple (Ananas comosus (L.) Merr) stems extract in decreasing the Enterococcus faecalis’s colonies Materials and Methods: This research was true experimental with the post test only control group design. The Queen pineapple stems extract tested its secondary metabolites contents. Enterococcus faecalis ATCC 29212 cultured in Brain Heart Infusion Broth synchronized with Mc Farland 0,5 solution and divided into seven groups (n=7). It used dilution series method, each BHIB tube given by the 100% extract (P1); 50% (P2); 25% (P3); 12,5% (P4) ; 6,25% (P5) ; 3,125% (P6) and without the extract (K(-)), incubated for 24 hours and streaked on Mueller Hinton Agar and incubated for 24 hours, then calculate the colonies’ amount of each group. Datas analyzed by Mann-Whitney U-Test because only 50% and 25% concentration can be calculated. Result: The resultshowed that the Queen pineapple stems contained flavonoids, tannins, alkaloids, saponins, also there were significant differences among the 50% and 25% concentration groups p<0.05. Conclusion: The Queen pineapple stems extract’s 50% concentration was effective to decrease the number of Enterococcus faecalis’s colonies.
Co-Authors Aprilia Arifzan Razak Arya Brahmanta Bambang Sucahyo Dwi Andriani, Dwi Dwi Setianingtyas Istien Wardani Linda Rochyani Nafiah Nora Lelyana Ratna Putri Saptaswari, Dianty Shinta Nurmaraya Febrianti Shintya Rizki Ayu Agitha Sularsih Twi Agnita Cevanti Vivin Ariestania Yufita Fitriani