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Padja, Tegar Abdiyanto
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology

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Antibacterial Activity Test of Etanol Extract of Black Sea Cucumber (Holothuria atra) against the Bacteria Staphylococcus aureus and Escherichia coli Husain, Fadli; Padja, Tegar Abdiyanto; Slamet, Nangsih Sulastri; Nur, Moh. Usman; Yunus, Fitriah Ayu Magfirah; Abubakar, Fadlun; Polontalo, Febriyanti
JURNAL INFO KESEHATAN Vol 23 No 1 (2025): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol23.Iss1.1337

Abstract

Black Sea cucumber (Holothuria atra) contain chemical compounds that has antibacterial activity. One of the bacteria that is pathogenic to humans and at risk of death are Staphylococcus aureus and Eschericia coli. The purpose of this study is to ascertain whether black sea cucumber ethanol extract in Pataomeme waters has antibacterial activity against the microorganisms E. coli and S. aureus. The type of research used is quantitative research with quasi laboratory experiments. Antibacterial activity test using disc diffusion method. Black Sea cucumber extract was made in 3 concentrations, consisted of 7.5%, 10% and 12.5%. The results showed that black sea cucumber extract has antibacterial activity seen through the inhibition zone formed. The maximum inhibition zone is at a concentration of 12.5% sea cucumber extract, which is 28.59 mm against S. aureus bacteria and 21.02 mm against E. coli bacteria. According to our results, sea cucumber may be a suitable marine source of antibacterial substances. To ascertain its potential use in other areas of medicine, more in vivo research must be conducted.
Co-Authors Abubakar, Fadlun Febriyanti Polontalo Husain, Fadli Nur, Moh. Usman Yunus, Fitriah Ayu Magfirah