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All Journal Agrica: Journal of Sustainable Dryland Agriculture
Khalimi , Khamdan
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Agriculture, Biological Sciences & Forestry Chemistry

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Uji Aktivitas Antijamur Saccharomyces cerevisiae terhadap Phytophthora palmivora Penyebab Busuk Buah Kakao Gargita, I Wayan Diksa; Khalimi , Khamdan; Pranatayana, Ida Bagus Gde
AGRICA Vol. 17 No. 2 (2024): December
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v17i2.4201

Abstract

The fungus Phytophthora palmivora is a phytopathogen that can cause cocoa pod rot disease. This work was conducted to determine the antifungal activity of Saccharomyces cerevisiae against P. palmivora ATCC39039 and identify the antifungal compounds produced by S. cerevisiae. The antifungal activity test of S. cerevisiae against P. palmivora ATCC39039 was performed using the dual culture method. Determination of the antifungal activity of S. cerevisiae extract on the growth of P. palmivora ATCC39039 was carried out using the poisoned food technique. The results indicated that S. cerevisiae successfully provided an inhibitory effect on the growth of P. palmivora ATCC39039 fungal colonies with an inhibition percentage of 92.93%. S. cerevisiae extract was also successful inhibiting the growth of P. palmivora ATCC39039 fungal colonies with the percentage inhibition ranging from 92.36% to 97.60%. S. cerevisiae extract contains thirteen compounds that have antifungal activity, namely Ethyl alcohol, 2-Methylbutan-1-ol, Isopentyl alcohol, Benzyl Carbinol, 1,2-Ethanediol, Acetoxyethane, 4-Hydroxyphenethyl alcohol, 4,4-Dimethyloxazolo, Acetoin, 2-Bromotetradecanoic acid, Dodecane,1-chloro, Octanoic acid ethyl ester, dan Diethylhexylphthalate. The results of this work provide information that S. cerevisiae can be utilized as a biological agent to control cocoa pod rot disease.
PENGENDALIAN PENYAKIT LAYU Fusarium PADA BAWANG MERAH (Allium ascolonicum L.) DENGAN Gliocladium sp. dan Trichoderma sp. Silvano, Jesis; Wirya, Gusti Ngurah Alit Susanta; Khalimi , Khamdan
AGRICA Vol. 18 No. 2 (2025): December
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v18i2.5388

Abstract

One of Indonesia's most valuable commodities is scallions, a type of onion known as Allium ascalonicum L. Wilt disease, caused by Fusarium sp., poses significant challenges to shallot production. Curled, light-green leaves, along with complete plant death, are symptoms of this disease, which reduces harvest yields. To manage Fusarium wilt in shallots, this research aims to assess the efficacy of antagonistic fungi, namely Trichoderma sp. and Gliocladium sp. The research used in vitro testing to determine how well the fungus inhibited the growth of Fusarium sp., and in vivo studies to assess how well the control worked in the real world. The active chemicals that were generated were identified by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. With an inhibitory power of 77%, Trichoderma sp. topped the list, followed by Gliocladium sp. with 75%.
Co-Authors GARGITA, I WAYAN DIKSA Gusti Ngurah Alit Susanta Wirya IDA BAGUS GDE PRANATAYANA Silvano, Jesis