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All Journal CERMIN: Jurnal Penelitian Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Prima Eksakta
Rahman, Ahmad Taufikur
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Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Mathematics Social Sciences

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ISOLASI DAN IDENTIFIKASI MIKROBA PADA SAMPEL MINUMAN YOGHURT DRINK DI ALUN-ALUN SITUBONDO Rahman, Ahmad Taufikur; Elhany, Nurul Avidhah; Fajar, Muhammad Thoifur Ibnu
PRIMA EKSAKTA Vol 2 No 2 (2025): Juli
Publisher : Fakultas Pertanian, Sains dan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/pe.v2i2.6763

Abstract

Food is everything that comes from biological sources and water, both processed and unprocessed, which is intended as food or drink for human consumption. In addition to being nutritious, food must also be free from hazardous materials in the form of chemical contaminants, pathogenic microbes and other materials. Microbes can contaminate food through water, dust, air, soil, food processing equipment and secretions from the intestines of humans or animals. Yoghurt is the result of milk fermentation with the help of lactic acid bacteria. This product is widely found in the community because of the benefits and nutrients contained in it. To find out the microbial content, both beneficial and not, microbiological tests must be carried out on yogurt drink samples that are sold freely in the community. Bacterial isolation is carried out by the pour cup method on MRSA media. Bacterial isolation is carried out by diluting the yogurt sample to a dilution of 10-6. The sample was then poured 1 ml into a Petri dish containing MRSA media. Then incubation was carried out for 48 hours at a temperature of 360C. The results of bacterial isolation were then counted and identified by observing the morphology of bacterial colonies that grew on MRSA media then continued with the gram staining test and observation of microscopic characteristics using a microscope. In addition, motility and biochemical tests of bacteria were also carried out (catalase test, citrate test, nitrate test, H2S test, MR test, VP test, starch hydrolysis test, Urea test and He test). The results of microbial identification in yogurt drink samples in Situbondo square are Streptococcus thermophilus and Lactobacillus sp. Both of these bacteria are lactic acid bacteria that play a role in the fermentation process and are not pathogenic to humans.
Pemanfaatan Limbah Kulit Kelapa Sebagai Media Tanam Dalam Budidaya Jamur Tiram Putih (Pleurotus ostreotus) dan Jamur Tiram Cokelat (Pleurotus pulmonarius) Ahmad Taufikur Rahman; Rahman, Ahmad Taufikur; Mahendra, Yusri; Eriyanto, Wahyu; Qomariyah, Rofi'atul; Elhany, Nurul Avidhah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 02 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.18.02.10784.%p

Abstract

One of the important biological resources in the Indonesian plantation sector is coconut. Coconut trees grow widely in several regencies in Indonesia, including Situbondo Regency. Based on data from the Situbondo Regency Statistics Agency (BPS), coconut plantation production in Situbondo Regency in 2023 reached 4.9 thousand tons. Coconut husks in Situbondo have been considered waste that is sold cheaply to prevent accumulation. Therefore, further processing or utilization is needed to provide greater productive value for coconut husks. Rarely used coconut husk waste can be an alternative to the hard-to-find sawdust media, thereby increasing productivity in mushroom cultivation, especially in the Situbondo region. This study used two types of oyster mushrooms: white oyster mushrooms and brown oyster mushrooms. Each received five treatments with three replications, resulting in a total of 15 white oyster mushroom bags and 15 brown oyster mushroom bags used. The treatment carried out was the addition of coconut fiber with different concentrations to the planting media. The planting medium that has the best influence on mycelium growth, fruit body growth time, fruit body wet weight is P1 (100% wood powder without the addition of coconut shells), while the best fruit cap diameter is obtained from the P4 treatment (75% wood powder + 25% coconut shell powder).   Keywords: Cultivation; Oyster Mushrom; Planting Media; Coconut shell
KEKERABATAN FENETIK VARIETAS MANGGA ARUM MANIS, ARUM MERAH, GADUNG, GARIFTA DAN MANALAGI DI SITUBONDO Fajar, Muhammad Thoifur Ibnu; Elhany, Nurul Avidhah; Rahman, Ahmad Taufikur
CERMIN: Jurnal Penelitian Vol 9 No 2 (2025): AGUSTUS - DESEMBER
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat - Universitas Abdurachman Saleh Situbondo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/cermin_unars.v9i2.7205

Abstract

Situbondo merupakan sentral penghasil mangga. Mangga popular meliputi varietas arum manis, arum merah, gadung, garifta dan manalagi. Tujuan penelitian menentukan hubungan kekerabatan fenetik varietas mangga dengan pendekatan fenotip pohon, daun, dan buah. Sampel penelitian diambil dari beberapa sentral mangga Situbondo. Parameter pengamatan terdiri dari 62 karakter, meliputi bentuk kanopi, diameter kanopi, tinggi tanaman, lingkar batang, kerapatan daun, panjang daun, lebar daun, panjang tangkai daun, warna daun, jenis daun, bentuk ujung daun, bentuk ujung daun, jenis tulang daun, bentuk daun, bentuk buah, panjang buah, diameter buah, warna buah, warna kulit buah, tekstur permukaan kulit buah, bau buah dan bentuk ujung buah. Metode penelitian menggunakan metode penelitian deskriptif kualitatif dan kuantitatif. Data skoring karakter fenotip kualitatif dan kuantitatif dianalisis menggunakan software MVSP dengan koefisien korelasi Jaccard Coefficient. Hasil dendogram terbagi menjadi tiga klaster dengan indeks similaritas 17%. Kekerabatan fenetik menunjukkan mangga arum manis berkerabat dekat dengan mangga arum merah, mangga garifta berkerabat dekat dengan mangga manalagi dan mangga gadung berkerabat jauh dengan mangga arum manis dan mangga arum merah. Keseragaman fenotip kelima varietas mangga diharapkan untuk pemuliaan dalam menghasilkan keturunan mangga berfenotip unggul.
Co-Authors Ahmad Taufikur Rahman Eriyanto, Wahyu Mahendra, Yusri Nurul Avidhah Elhany Qomariyah, Rofi'atul Thoifur Ibnu Fajar, Muhammad