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All Journal Journal of Biomedical and Techno Nanomaterials Journal of Early Childhood Education and Teaching
Ahmed, Shakib
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Biochemistry, Genetics & Molecular Biology Social Sciences Other

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Development of Interactive Smart Poster Media for Sexual Education Introduction to Children Aged 5–6 Years Syaharra, Salsya Iffana; Sari, Meliana; Ahmed, Shakib
Journal of Early Childhood Education and Teaching (JECET) Vol. 1 No. 1 (2025)
Publisher : CV Berkah Syahdin Trust

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64840/jecet.v1i1.15

Abstract

Purpose – A lack of interactive and developmentally appropriate educational tools has limited young children's understanding of basic sexual education. This study aims to develop an interactive smart poster as a responsive educational medium tailored for children aged 5–6 years to introduce foundational concepts of sexual education in an engaging and age-appropriate manner. Method – This research employed a Research and Development (R&D) design using the 4D model (Define, Design, Develop, Disseminate) by Thiagarajan, focusing on the development phase. Data were gathered through expert validation and practitioner assessments using standardized instruments to evaluate media validity and practicality. Validity assessments included aspects of visual design, language clarity, and media functionality. Practicality was measured based on the content’s relevance and its applicability in early childhood classroom settings. The instruments were reviewed by two content experts and two early childhood educators. Findings – The interactive smart poster achieved a validity score of 90.59%, indicating a high level of validity, and a practicality score of 90.71%, denoting high practicality. These results support the media’s suitability and potential effectiveness in introducing basic sexual education concepts to young learners. Research implications/limitations – The study is limited to the developmental stage and initial feasibility testing. Future studies should explore the long-term impact, scalability, and integration of the smart poster into diverse educational contexts. Originality/value – This study offers an innovative approach by integrating responsive visual media into early childhood sexual education. The use of smart posters represents a novel contribution to pedagogical tools aimed at fostering early awareness in a safe, engaging, and developmentally appropriate manner.
TARGETED GENE SILENCING OF KRAS ONCOGENES IN PANCREATIC CANCER USING SIRNA-LOADED GOLD NANOPARTICLES Ahmed, Shakib; Islam, Zahidul; El Balqis, Fatimah
Journal of Biomedical and Techno Nanomaterials Vol. 2 No. 4 (2025)
Publisher : Yayasan Adra Karima Hubbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70177/jbtn.v2i4.2516

Abstract

Pancreatic cancer, predominantly driven by mutations in the KRAS oncogene, remains one of the most lethal malignancies due to its resistance to conventional therapies. RNA interference (RNAi) using small interfering RNA (siRNA) presents a powerful strategy to silence oncogenes, but its clinical application is liited by the poor stability and inefficient delivery of naked siRNA. This study aimed to develop and validate a targeted nanodelivery system using gold nanoparticles (AuNPs) to efficiently deliver KRAS-specific siRNA and induce potent gene silencing in pancreatic cancer cells. A nanoconjugate was synthesized by attaching thiol-modified siRNA targeting the G12D-mutant KRAS gene to PEGylated gold nanoparticles. The physicochemical properties of the siRNA-AuNPs were characterized. The platform’s efficacy was evaluated in vitro using the PANC-1 human pancreatic cancer cell line. KRAS expression was quantified via qRT-PCR and Western blot, while cellular viability and apoptosis were assessed using MTT and flow cytometry assays, respectively. The synthesized siRNA-AuNPs exhibited excellent stability and were efficiently internalized by the cancer cells. This targeted delivery resulted in a significant downregulation of KRAS mRNA and protein expression by over 75% (p < 0.01) compared to controls. Consequently, this oncogene silencing led to a substantial inhibition of cancer cell proliferation and a marked increase in apoptosis. Gold nanoparticles serve as a highly effective and robust vector for the targeted delivery of siRNA. This nanomedicine platform successfully silences the critical KRAS oncogene, inducing cell death in pancreatic cancer cells and representing a promising new avenue for targeted cancer therapy.
Co-Authors El Balqis, Fatimah Islam, Zahidul Meliana Sari, Meliana Syaharra, Salsya Iffana